FastQCFastQC Report
Mon 6 Jun 2016
ERR522881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19852851
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT567310.28575744612197007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433570.2183918067989328No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT414070.2085695399617919No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279740.14090671410368213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG317000.050.7167242
GTACCTG376450.043.4668771
ACCTGGG380750.041.9539153
CCTGGGG367350.030.9879744
GTACATG888450.024.7014831
TATAACG17550.024.3823552
CATGGGG553000.024.368544
TACATGG912900.023.6377562
TGGGGGG326450.023.407096
ATGGGGG341900.022.8553775
CTGGGGG238700.022.8487285
ACATGGG934300.022.5329273
GTACCCG40650.020.248311
GAGTACT501700.019.40170912-13
GTATCAA1164500.019.0034071
GTACACG47550.018.793791
ATAACGC22900.018.2755893
AGTACTT532350.017.58734112-13
TCAACGC1271300.017.079864
CAACGCA1279950.016.9635775
Produced by FastQC (version 0.11.5)