FastQCFastQC Report
Mon 6 Jun 2016
ERR522881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19852851
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT508020.2558927178771452No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT422910.21302230092796245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415050.20906317183360715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270810.13640861959826325No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG329400.050.306622
GTACCTG381550.043.891081
ACCTGGG390400.041.7564663
CCTGGGG360650.032.6530954
CTGGGGG226150.024.18725
TGGGGGG299900.023.8801866
GTACATG881550.022.9593721
CATGGGG544200.022.6586764
TACATGG905150.022.118492
ATGGGGG324250.022.1013645
ACATGGG926100.021.0022953
GTACCCG41550.019.9147761
TATAACG17250.019.8884142
GAGTACT508050.018.35637712-13
GTACACG42400.017.8522871
GTATCAA1184550.017.7931
AGTACTT540400.016.93131812-13
TAACGCA23350.016.7039994
TATCACG21600.016.535842
TACCCGG49600.016.3919582
Produced by FastQC (version 0.11.5)