##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522881_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19852851
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.236062367062544	33.0	31.0	34.0	30.0	34.0
2	32.367378569455845	34.0	31.0	34.0	30.0	34.0
3	32.567621748634494	34.0	31.0	34.0	30.0	35.0
4	35.65180804510143	37.0	35.0	37.0	33.0	37.0
5	35.5787067056515	36.0	35.0	37.0	33.0	37.0
6	35.698272505042226	37.0	35.0	37.0	33.0	37.0
7	35.53670598746749	37.0	35.0	37.0	33.0	37.0
8	35.52238250314778	36.0	35.0	37.0	33.0	37.0
9	36.814595243776324	38.0	37.0	39.0	33.0	39.0
10-11	36.61770163388624	37.5	35.0	39.0	33.0	39.0
12-13	36.62000037173502	37.5	35.0	39.0	33.0	39.0
14-15	37.80727586682638	38.0	37.0	41.0	33.0	41.0
16-17	37.623107003623815	38.0	36.5	41.0	33.0	41.0
18-19	37.51098419063338	38.0	36.0	41.0	32.0	41.0
20-21	37.52147036715281	38.0	36.5	41.0	33.0	41.0
22-23	37.38248393140108	38.0	36.0	41.0	33.0	41.0
24-25	37.30698134993307	38.0	36.5	41.0	32.0	41.0
26-27	36.99371047513529	38.0	36.0	40.0	32.5	41.0
28-29	36.96349400899649	38.0	36.0	40.0	32.0	41.0
30-31	36.73465989847	38.0	35.5	40.0	31.5	41.0
32-33	36.70825887929144	37.5	36.0	40.0	30.5	41.0
34-35	36.49117690955319	38.0	35.5	40.0	30.5	41.0
36-37	36.28137875512187	37.0	35.5	40.0	30.5	41.0
38-39	36.24746876405812	37.0	35.5	40.0	30.0	41.0
40-41	36.45151099456697	37.5	35.5	40.0	31.0	41.0
42-43	36.50471108658398	37.5	35.5	40.0	31.0	41.0
44-45	36.33820275989579	37.0	35.0	40.0	30.5	41.0
46-47	36.252822050596166	37.0	35.5	40.0	30.0	41.0
48-49	36.055305532691506	38.0	35.0	40.0	30.0	41.0
50-51	35.89490698338491	38.0	34.5	40.0	29.5	41.0
52-53	35.90921263651251	37.5	35.0	40.0	29.0	41.0
54-55	35.70817788336798	37.5	34.5	40.0	28.0	41.0
56-57	35.453332596915175	37.0	35.0	40.0	28.0	41.0
58-59	35.258527301695864	37.0	34.5	39.5	27.5	41.0
60-61	35.062051037405155	37.0	34.0	39.0	28.0	41.0
62-63	34.81431810473971	36.5	34.0	39.0	27.0	41.0
64-65	34.62214489999447	37.0	33.5	38.5	26.0	40.5
66-67	34.10937590273558	35.5	33.5	38.0	26.0	40.0
68-69	33.966193344220436	36.5	33.0	37.0	26.0	40.0
70-71	33.54537585055164	35.0	33.0	37.0	25.5	39.5
72-73	33.31259208060344	35.0	33.0	37.0	25.5	39.0
74-75	33.13019067135496	35.0	32.5	37.0	25.0	39.0
76-77	31.53841433152347	34.0	30.0	36.0	23.0	37.0
78-79	32.65466204325011	35.0	32.0	36.5	24.5	37.0
80-81	32.765624090968096	35.0	32.0	36.0	25.0	37.5
82-83	32.67161784471158	35.0	32.0	37.0	25.0	37.0
84-85	32.34763364717743	35.0	32.0	36.0	24.5	36.5
86-87	32.23836966791319	35.0	32.0	36.0	25.0	36.0
88-89	32.20286632887135	35.0	32.0	36.0	24.0	37.0
90-91	31.969412856621954	34.5	32.0	35.0	23.5	36.5
92-93	31.76484878670575	35.0	31.5	35.0	22.0	36.5
94-95	31.903441274001402	35.0	31.0	35.0	23.0	36.5
96-97	31.626561797094027	35.0	31.0	35.0	22.0	37.0
98-99	31.481460672827293	34.0	31.0	35.0	20.5	36.0
100	30.239132253599244	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.06304108926426721
1101	2	-0.13853667377643575
1101	3	-0.1047834352732977
1101	4	-0.06350686515747839
1101	5	-0.14801780172516033
1101	6	-0.07670847062645691
1101	7	-0.06657700512485576
1101	8	-0.20681755042552652
1101	9	-0.1594471013750436
1101	10-11	-0.11913297550292867
1101	12-13	-0.11451983946729172
1101	14-15	-0.2702220670091222
1101	16-17	-0.24369747296462663
1101	18-19	-0.39312329853041206
1101	20-21	-0.31047067513268445
1101	22-23	-0.825484688026421
1101	24-25	-0.28453979440490684
1101	26-27	-0.2894052623709058
1101	28-29	-0.3410159958059822
1101	30-31	-0.36570819911954544
1101	32-33	-0.27601542746580066
1101	34-35	-0.4024350762169675
1101	36-37	-0.43752744494593543
1101	38-39	-0.3411508182588179
1101	40-41	-0.3662664733457106
1101	42-43	-0.33298055069953136
1101	44-45	-0.4293638255268064
1101	46-47	-0.2926677674852627
1101	48-49	-0.4028776952593631
1101	50-51	-0.3798293070915477
1101	52-53	-0.3828583967518284
1101	54-55	-0.38135490452662424
1101	56-57	-0.49557398715923284
1101	58-59	-0.4063008854605812
1101	60-61	-0.6019481100669211
1101	62-63	-0.4431125065648942
1101	64-65	-0.41497774287822864
1101	66-67	-0.4343748941698351
1101	68-69	-0.453763552725313
1101	70-71	-0.4772848553764888
1101	72-73	-0.5262907149198668
1101	74-75	-0.4620917372310416
1101	76-77	-0.5448106089793647
1101	78-79	-0.47740111579178546
1101	80-81	-0.3656743515184999
1101	82-83	-0.34102965631795
1101	84-85	-0.37603856131071467
1101	86-87	-0.48207219091180065
1101	88-89	-0.5250464735263911
1101	90-91	-0.5585302144933877
1101	92-93	-0.4469778798280686
1101	94-95	-0.5067057687150545
1101	96-97	-0.6319444173671727
1101	98-99	-0.6332113155735186
1101	100	-0.5220152571975234
1102	1	-0.011535004442009722
1102	2	-0.07721632481651852
1102	3	-0.09822302396310789
1102	4	-0.12006622403342249
1102	5	-0.11452636401750738
1102	6	-0.14410877315251014
1102	7	-0.06876116294441204
1102	8	-0.13230983310252498
1102	9	-0.13135231144500636
1102	10-11	-0.17482087055532247
1102	12-13	-0.13037179191539394
1102	14-15	-0.2616351662752052
1102	16-17	-0.2549829415464444
1102	18-19	-0.25799548663438543
1102	20-21	-0.3055453825396981
1102	22-23	-0.2173335412392987
1102	24-25	-0.17042316595009765
1102	26-27	-0.25160712049761
1102	28-29	-0.2820819811667974
1102	30-31	-0.32337584942924025
1102	32-33	-0.25273018859376606
1102	34-35	-0.28922016513548954
1102	36-37	-0.3530023400031297
1102	38-39	-0.36204398059036436
1102	40-41	-0.34162215152647235
1102	42-43	-0.4183587176058552
1102	44-45	-0.312283393234253
1102	46-47	-0.2510436703673449
1102	48-49	-0.33245107241257443
1102	50-51	-0.1860077750087612
1102	52-53	-0.3126870128188415
1102	54-55	-0.2985471115269931
1102	56-57	-0.3140628482392387
1102	58-59	-0.33947341722893043
1102	60-61	-0.4121355261515518
1102	62-63	-0.4734641200200471
1102	64-65	-0.43756661234623095
1102	66-67	-0.4828406428284353
1102	68-69	-0.36960506057671694
1102	70-71	-0.38417298873020655
1102	72-73	-0.41314764323092135
1102	74-75	-0.4911075251717776
1102	76-77	-0.4014490546689231
1102	78-79	-0.444096012984069
1102	80-81	-0.4012616391033319
1102	82-83	-0.35996431773727267
1102	84-85	-0.4654303929267769
1102	86-87	-0.42692913519299225
1102	88-89	-0.47324025869545494
1102	90-91	-0.5439158717305972
1102	92-93	-0.4745589942302324
1102	94-95	-0.6431680892710787
1102	96-97	-0.6565577694986828
1102	98-99	-0.7708934813963175
1102	100	-0.8258977815698749
1103	1	-0.0955169007073664
1103	2	-0.12327765936002777
1103	3	-0.23230569929672384
1103	4	-0.048677015221400666
1103	5	-0.14680489232622307
1103	6	-0.15993282775248474
1103	7	-0.1315974831993998
1103	8	-0.11250195981092759
1103	9	-0.18907262741254982
1103	10-11	-0.2678494340675712
1103	12-13	-0.2080332563762397
1103	14-15	-0.201312024100595
1103	16-17	-0.2909118380451048
1103	18-19	-0.34189745111589787
1103	20-21	-0.27488384635405083
1103	22-23	-0.3207055605532645
1103	24-25	-0.25426186592289923
1103	26-27	-0.29288104374018076
1103	28-29	-0.25776476142799254
1103	30-31	-0.4314951691223925
1103	32-33	-0.330517116332409
1103	34-35	-0.3759272914780212
1103	36-37	-0.45715580107968634
1103	38-39	-0.3770820824091956
1103	40-41	-0.40594410755913657
1103	42-43	-0.3859034208941381
1103	44-45	-0.28855283034303625
1103	46-47	-0.4210172995067367
1103	48-49	-0.4115478702661193
1103	50-51	-0.4596990669700389
1103	52-53	-0.4001986019496968
1103	54-55	-1.1873342975316135
1103	56-57	-0.46007979787761855
1103	58-59	-0.4076176050898255
1103	60-61	-0.39340199583173074
1103	62-63	-0.38694880974644263
1103	64-65	-0.3439943930050049
1103	66-67	-0.3295077660831467
1103	68-69	-0.32621248766046307
1103	70-71	-0.43942134134088207
1103	72-73	-0.496585500421034
1103	74-75	-0.5806400309283717
1103	76-77	-0.5123880392068045
1103	78-79	-0.4447774130337123
1103	80-81	-1.2467625426562776
1103	82-83	-0.37250242147232626
1103	84-85	-0.24789766345936215
1103	86-87	-0.2828113886785246
1103	88-89	-0.33365255392657645
1103	90-91	-0.2951768503842729
1103	92-93	-0.3071076190203037
1103	94-95	-0.4146862633392878
1103	96-97	-0.5039699379330429
1103	98-99	-0.4719481399473615
1103	100	-0.4823026068272682
1104	1	-0.06341654985025258
1104	2	-0.2000077388587158
1104	3	-0.13951287314640837
1104	4	-0.052475405995274116
1104	5	-0.04803599428572625
1104	6	-0.22423040859244026
1104	7	-0.1931525491049868
1104	8	-0.07477892098552985
1104	9	-0.1631092248244883
1104	10-11	-0.14121111126862473
1104	12-13	-0.13618600266935488
1104	14-15	-0.28734151486971626
1104	16-17	-0.3054700653166478
1104	18-19	-0.2905501435122986
1104	20-21	-0.32223527685915343
1104	22-23	-0.2560200610060974
1104	24-25	-0.16857525499570158
1104	26-27	-0.23355399328357151
1104	28-29	-0.3234346299661084
1104	30-31	-0.3473835822536131
1104	32-33	-0.2759958488308243
1104	34-35	-0.3076904548199906
1104	36-37	-0.37929374895339407
1104	38-39	-0.23100456924726132
1104	40-41	-0.16912687158919226
1104	42-43	-0.2032318617483213
1104	44-45	-0.28158944441771894
1104	46-47	-0.2636692412375723
1104	48-49	-0.3013293393025265
1104	50-51	-0.24822878132334125
1104	52-53	-0.3114725237024132
1104	54-55	-0.34889244477653136
1104	56-57	-0.25448279887229575
1104	58-59	-0.32212762797445293
1104	60-61	-0.40657002461919234
1104	62-63	-0.399371539250005
1104	64-65	-0.6056732536556027
1104	66-67	-0.45569212715829366
1104	68-69	-0.3191517308779126
1104	70-71	-0.29424671805966796
1104	72-73	-0.29200671169017767
1104	74-75	-0.36346428740794323
1104	76-77	-0.3337692096071798
1104	78-79	-0.2855876411881795
1104	80-81	-0.30051879217297994
1104	82-83	-0.28283202897290494
1104	84-85	-0.9149583932501315
1104	86-87	-0.3661159412424624
1104	88-89	-0.3981066390108552
1104	90-91	-0.40175381284031886
1104	92-93	-0.3200181635572328
1104	94-95	-0.32272106275097556
1104	96-97	-0.2786471987057624
1104	98-99	-0.34259867815886125
1104	100	-1.490343582279884
1105	1	-0.018711768688973507
1105	2	-0.15129972890326826
1105	3	-0.18416839117134032
1105	4	-0.08314765006092273
1105	5	-0.12052849165130652
1105	6	-0.11297758760496635
1105	7	-0.13157960634362098
1105	8	-0.19729483266542758
1105	9	-0.1458573411024986
1105	10-11	-0.1995986612574825
1105	12-13	-0.15912733890867514
1105	14-15	-0.2391465637562149
1105	16-17	-0.2520208629190748
1105	18-19	-0.29025331745875604
1105	20-21	-0.24608087481015417
1105	22-23	-0.2735306448185071
1105	24-25	-0.1450872964879153
1105	26-27	-0.23400759790270342
1105	28-29	-0.20995223165008525
1105	30-31	-0.274707114990008
1105	32-33	-0.20757298888602804
1105	34-35	-0.30956009944435436
1105	36-37	-0.3079553095144192
1105	38-39	-0.34980781802384797
1105	40-41	-0.25217451226672694
1105	42-43	-0.1998409243025563
1105	44-45	-0.37655620565885783
1105	46-47	-0.36140140069667837
1105	48-49	-0.3667725856709936
1105	50-51	-0.2741970981043025
1105	52-53	-0.40253743533997266
1105	54-55	-1.2966149481545628
1105	56-57	-0.2816805700305167
1105	58-59	-0.3035385812310665
1105	60-61	-0.25649133218888664
1105	62-63	-0.25114295408661036
1105	64-65	-0.26079649391261484
1105	66-67	-0.3283637507242858
1105	68-69	-0.17317058009717812
1105	70-71	-0.14635887602982223
1105	72-73	-0.23523139477876498
1105	74-75	-0.2631554194024588
1105	76-77	-0.2999542013055354
1105	78-79	-0.25795073654041545
1105	80-81	-0.20960647899662632
1105	82-83	-0.176047101285981
1105	84-85	-0.18027767160767638
1105	86-87	-0.1634521713956758
1105	88-89	-0.2867807081711007
1105	90-91	-0.3063029909820969
1105	92-93	-0.19306354335396136
1105	94-95	-0.2621373163053171
1105	96-97	-0.2716008099541618
1105	98-99	-0.15261295300875943
1105	100	-0.09155552472481787
1106	1	-0.029000795912558885
1106	2	-0.12261291296460541
1106	3	-0.09605448842989261
1106	4	-0.0750584136460759
1106	5	-0.09788899321857514
1106	6	-0.13206754248675168
1106	7	-0.06879149762910686
1106	8	-0.1896041230637593
1106	9	-0.14039405545436523
1106	10-11	-0.1817156339712085
1106	12-13	-0.16653202178940063
1106	14-15	-0.18131916594097675
1106	16-17	-0.16388437969940384
1106	18-19	-0.24148020244049917
1106	20-21	-0.24092845233930404
1106	22-23	-0.18147397921190134
1106	24-25	-0.1556407647060425
1106	26-27	-0.10713968036101562
1106	28-29	-0.1043442405285333
1106	30-31	-0.18622945647424416
1106	32-33	-0.04083775462758865
1106	34-35	-0.087382792976058
1106	36-37	-0.18388844493085088
1106	38-39	-0.20741955881904062
1106	40-41	-0.1903909321385342
1106	42-43	-0.2003249544973542
1106	44-45	-0.1706961858205318
1106	46-47	-0.253119634400754
1106	48-49	-0.23643176574319824
1106	50-51	-0.2045727198578291
1106	52-53	-0.25358210791853963
1106	54-55	-0.3243885081903741
1106	56-57	-0.2306864613724926
1106	58-59	-0.25795220993430235
1106	60-61	-0.2648808038473973
1106	62-63	-0.27498476467338406
1106	64-65	-0.1852927301184053
1106	66-67	-0.12498731054743928
1106	68-69	-0.08905415545159912
1106	70-71	-0.09848417583818758
1106	72-73	-0.14387905356038289
1106	74-75	-0.1901583732746488
1106	76-77	-0.18856272181615097
1106	78-79	-0.11389774011664144
1106	80-81	-0.05003335176089507
1106	82-83	-0.10257575257543294
1106	84-85	-0.04147628695867667
1106	86-87	0.07447677140962128
1106	88-89	-0.07921335788476824
1106	90-91	-0.09037395870346998
1106	92-93	-0.021628656517190592
1106	94-95	0.0011173273902080894
1106	96-97	0.009502679854605844
1106	98-99	-0.10180343096390487
1106	100	-0.08403551919208496
1107	1	-0.027279481333650324
1107	2	-0.0822308057119514
1107	3	-0.08032022833742047
1107	4	-0.039166550729042626
1107	5	-0.12252868634941905
1107	6	-0.1327581207793358
1107	7	-0.16465807554556733
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2315	1	0.07649066816495775
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2315	4	-0.09207279141551794
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2315	100	0.2709222119124135
2316	1	0.013224723181323839
2316	2	-0.13730316882772797
2316	3	-0.11990297270858008
2316	4	-0.01697544444768795
2316	5	-0.06706434545302642
2316	6	-0.05274716826352943
2316	7	-0.01225624382369972
2316	8	5.417358389863125E-4
2316	9	-0.1332991468042124
2316	10-11	-4.8178642283858153E-4
2316	12-13	0.02030273046914033
2316	14-15	0.05457487037673303
2316	16-17	-1.2855979139435618
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2316	28-29	0.06781216187997785
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2316	34-35	0.20852171613363168
2316	36-37	0.13347870894731528
2316	38-39	0.11279235542583876
2316	40-41	0.044303668383292916
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2316	52-53	0.09860455127479639
2316	54-55	0.16367302876354728
2316	56-57	0.09706110661564082
2316	58-59	0.1016254298460808
2316	60-61	-1.194835794026332
2316	62-63	0.1127875218793335
2316	64-65	0.12232583470815683
2316	66-67	-0.05680597245982
2316	68-69	0.20053931299131733
2316	70-71	0.15508284081829515
2316	72-73	0.25721251529621014
2316	74-75	0.12129614674997669
2316	76-77	-0.13003492241822556
2316	78-79	-0.2137004401328042
2316	80-81	-0.0804227568940945
2316	82-83	-0.1334926633577993
2316	84-85	-0.6404736265952806
2316	86-87	-0.02285048317483529
2316	88-89	0.037201664252243916
2316	90-91	0.16887539363547432
2316	92-93	0.2994609315956218
2316	94-95	0.2377915124683092
2316	96-97	0.2299999405598463
2316	98-99	0.3049320641019726
2316	100	0.09384685967645723
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	3.0
11	17.0
12	109.0
13	609.0
14	2156.0
15	5658.0
16	12157.0
17	22413.0
18	36133.0
19	52846.0
20	72567.0
21	97364.0
22	126808.0
23	152805.0
24	175712.0
25	201451.0
26	237526.0
27	286170.0
28	348148.0
29	426021.0
30	523184.0
31	647213.0
32	810545.0
33	1050293.0
34	1493957.0
35	2636607.0
36	4129490.0
37	3038048.0
38	2768519.0
39	498080.0
40	239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.62331311536848	17.603462383382944	12.420903287019696	24.352321214228876
2	16.69170251438363	20.117537423676517	38.52762314838711	24.66313691355275
3	20.00123810932747	24.981021617499675	28.719456968674173	26.298283304498682
4	13.669699673078844	15.616729912996147	35.505895163699584	35.20767525022542
5	14.350046454486314	36.451788957356825	33.25061269802972	15.947551890127142
6	32.69002522609977	34.40978325984515	17.889848667075576	15.01034284697951
7	29.480945478499887	31.159173291367225	20.94406409317515	18.415817136957738
8	27.591672283831663	33.642525063670995	19.62645340681267	19.139349245684674
9	27.676891344220532	15.704318739912972	19.35819192920956	37.26059798665693
10-11	26.181266165814982	24.977464192798514	27.390608905018677	21.450660736367826
12-13	26.513800338859145	22.576925768852206	27.515337074889445	23.393936817399204
14-15	23.782654716001257	24.090399345866658	25.384943376670694	26.742002561461398
16-17	23.61518850204008	26.870843996436626	25.959917301222635	23.55405020030066
18-19	23.42294190754085	26.286629922811812	27.512471953954602	22.77795621569273
20-21	24.11373062270727	25.435360550510982	27.33102623962716	23.119882587154592
22-23	23.88193688285167	25.157455889004133	27.255371724444778	23.70523550369942
24-25	23.822700148662886	25.247287264420333	27.21065486598617	23.719357720930613
26-27	23.67548336417156	25.188766850033524	27.540537752677295	23.595212033117622
28-29	23.52653980479539	25.776052226517628	27.364884369112445	23.332523599574532
30-31	24.133708158960907	25.32974879816741	27.24839396517944	23.288149077692243
32-33	23.879025489434095	25.46321179065187	27.261070447776248	23.39669227213779
34-35	24.036511728399603	25.492042081812716	27.24102800426738	23.2304181855203
36-37	24.030036623320207	25.464380315126583	27.258927538778284	23.246655522774926
38-39	23.925031956426427	25.7237017349156	27.10249761837982	23.248768690278155
40-41	23.84702307768546	25.587944943399833	27.10421661765126	23.460815361263446
42-43	23.68953520722845	25.667156588922047	26.8120805690305	23.831227634819
44-45	23.60888540567247	25.60824474505153	26.98447876480552	23.79839108447048
46-47	23.70106145547899	25.597276046561664	26.882314491894665	23.819348006064683
48-49	23.757717025686386	25.690136356298083	26.854641052114335	23.697505565901196
50-51	23.411434506710776	25.97967116184574	26.94053187363065	23.668362457812826
52-53	23.603034561985286	26.073948531999857	26.450004380240326	23.873012525774527
54-55	23.340260994079735	25.768802476136038	26.774405950173584	24.116530579610647
56-57	23.64672067603897	25.771824758174322	26.51616881026752	24.065285755519188
58-59	23.744064660759566	25.746691456259978	26.63780905991561	23.871434823064842
60-61	23.885860156772747	25.879539348621233	26.796956423306007	23.437644071300014
62-63	23.518700739063583	25.969349450267153	26.76166993866112	23.75027987200814
64-65	23.394780204039357	25.938695006935426	26.64343735105209	24.023087437973132
66-67	23.481997329728916	25.797736686851025	26.474172284971704	24.24609369844836
68-69	23.25665537734845	25.899479104687178	26.56996764316436	24.273897874800014
70-71	23.427658034839006	25.809789810065137	26.528935002941147	24.233617152154714
72-73	23.370861095949092	26.001265046415927	26.352782124895153	24.275091732739828
74-75	23.232699048675016	26.36101275488108	26.164609251882208	24.24167894456169
76-77	23.219019006050033	25.865418442997363	26.332386550246568	24.583176000706032
78-79	23.18181117547695	25.93496189278479	26.433119623440206	24.450107308298055
80-81	23.300885170384525	26.143805236574536	26.080187149000235	24.4751224440407
82-83	23.28648440847706	26.071982526892214	26.218406090180572	24.42312697445015
84-85	23.209682006344096	26.29586598072432	25.96088025126549	24.533571761666096
86-87	23.060500645967743	26.315063869042167	26.051259392171012	24.57317609281908
88-89	22.83337932179461	26.131338814271395	26.119005545110348	24.916276318823645
90-91	23.29379801741889	25.930061651640163	26.038109619689337	24.73803071125161
92-93	23.233308388864433	26.142326425118274	25.98650429767602	24.63786088834127
94-95	23.041300202598173	25.940250023113816	26.056313995709786	24.96213577857823
96-97	22.780437902582513	25.91656939908481	26.330717233317074	24.972275465015606
98-99	22.902626523170824	25.99443039293253	25.971146461494726	25.131796622401925
100	22.71963057730758	25.911938730700847	26.046942262190136	25.321488429801438
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4456.0
1	3378.0
2	5509.5
3	7865.0
4	5362.5
5	4157.0
6	5927.5
7	10295.5
8	15617.0
9	18252.5
10	16761.0
11	14769.5
12	15284.5
13	16626.5
14	18245.5
15	19696.0
16	20910.5
17	22337.5
18	23885.5
19	25469.0
20	27251.0
21	30076.5
22	33603.0
23	38497.0
24	44896.5
25	52113.0
26	62257.0
27	75416.5
28	89929.0
29	108181.5
30	130309.0
31	152132.0
32	179178.0
33	209676.5
34	238760.5
35	271193.5
36	313983.5
37	357571.5
38	394062.0
39	430789.0
40	461014.0
41	488023.0
42	521695.0
43	562705.5
44	602343.5
45	643503.5
46	693039.5
47	760993.0
48	859612.0
49	905846.5
50	908358.0
51	900159.5
52	865811.0
53	846208.0
54	830687.0
55	793292.5
56	731395.0
57	667205.5
58	606481.0
59	535381.0
60	455236.0
61	378644.0
62	310646.5
63	246250.5
64	185838.5
65	139461.5
66	104532.5
67	80424.0
68	62512.5
69	47393.0
70	36696.0
71	28457.0
72	22095.5
73	17172.5
74	13327.0
75	9738.0
76	6764.0
77	4218.0
78	2462.5
79	1598.5
80	1062.0
81	679.0
82	453.5
83	301.0
84	206.0
85	138.5
86	94.5
87	66.0
88	54.0
89	45.0
90	30.5
91	21.5
92	13.0
93	3.0
94	3.5
95	2.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04507161213268562
2	0.009983452754468363
3	0.0
4	2.6192711565709123E-4
5	4.3318715281749707E-4
6	0.0
7	2.0148239665930098E-5
8	6.548177891427281E-5
9	0.0
10-11	5.0370599164825245E-6
12-13	2.997050650307102E-4
14-15	0.01241131563421294
16-17	0.0016143777032326492
18-19	3.777794937361893E-5
20-21	8.462260659690641E-4
22-23	7.454848676394137E-4
24-25	0.004475427735794723
26-27	0.016614742134517607
28-29	0.01362524707408523
30-31	0.01017737956125294
32-33	0.007200477150611768
34-35	0.009091893149250956
36-37	0.005132764054895693
38-39	0.004125352071599188
40-41	0.005533210318256053
42-43	0.007061958002908499
44-45	0.007389366897479863
46-47	0.01598007258504081
48-49	0.01833237956603815
50-51	0.017690154426686627
52-53	0.011796794324402072
54-55	0.003986832923895918
56-57	0.0037324613981135502
58-59	0.01031589870895621
60-61	0.002440455529535783
62-63	1.3096355782854562E-4
64-65	3.198533046966403E-4
66-67	9.31856084549267E-4
68-69	6.800030887251409E-5
70-71	2.2666769624171362E-4
72-73	0.003097791848636753
74-75	0.005628914456669221
76-77	0.005374542930886853
78-79	0.0035511272411201797
80-81	3.2740889457136404E-5
82-83	8.663743056349941E-4
84-85	3.2740889457136404E-4
86-87	4.0044626336036064E-4
88-89	2.2666769624171362E-4
90-91	4.7600216210759856E-4
92-93	4.3570568277573834E-4
94-95	3.2740889457136404E-5
96-97	0.002009786906676527
98-99	5.238542313141825E-4
100	1.8637121690985342E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9852851E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.13203900118469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.47377557322801	26.742795880036862
2	17.39087782049485	14.654262851958539
3	7.492025661632236	9.469629521212978
4	3.7936322279366044	6.393338439343048
5	2.184811980040613	4.602529177666333
6	1.3472345760856377	3.4057043822030764
7	0.9100665300188582	2.684007097549914
8	0.6431812707139307	2.1678830706040686
9	0.44770482207876244	1.6976445322356783
>10	2.148679795819425	15.341281448258663
>50	0.10436673426287278	3.000134051775263
>100	0.05703411745169804	4.53033441853593
>500	0.004171117742077732	1.1952727502371967
>1k	0.0020278833204003368	1.47668054674211
>5k	2.588773731505441E-4	0.8062386853560662
>10k+	1.510118010044841E-4	1.8322631462844925
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50802	0.2558927178771452	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	42291	0.21302230092796245	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41505	0.20906317183360715	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27081	0.13640861959826325	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5111179749447573E-5
2	0.0	0.0	0.0	0.0	1.5111179749447573E-5
3	0.0	0.0	0.0	0.0	1.5111179749447573E-5
4	0.0	0.0	0.0	5.0370599164825245E-6	2.0148239665930098E-5
5	0.0	0.0	0.0	5.0370599164825245E-6	2.0148239665930098E-5
6	0.0	0.0	0.0	1.5111179749447573E-5	6.548177891427282E-5
7	0.0	0.0	0.0	1.5111179749447573E-5	6.548177891427282E-5
8	0.0	0.0	0.0	1.5111179749447573E-5	6.548177891427282E-5
9	0.0	0.0	0.0	1.5111179749447573E-5	8.563001858020292E-5
10-11	0.0	0.0	0.0	1.5111179749447573E-5	8.814854853844418E-5
12-13	0.0	0.0	0.0	2.5185299582412622E-5	9.822266837140923E-5
14-15	0.0	0.0	0.0	2.7703829540653883E-5	1.5866738736919952E-4
16-17	0.0	0.0	0.0	3.777794937361893E-5	1.8888974686809466E-4
18-19	0.0	2.5185299582412622E-6	0.0	5.288912912306651E-5	2.0148239665930098E-4
20-21	0.0	2.0148239665930098E-5	0.0	1.4103767766151067E-4	2.11556516492266E-4
22-23	0.0	2.5185299582412622E-5	0.0	3.9792773340211943E-4	2.518529958241262E-4
24-25	0.0	2.5185299582412622E-5	0.0	9.620784440481621E-4	2.8207535532302134E-4
26-27	0.0	2.5185299582412622E-5	0.0	0.0018763048188897404	2.8711241523950387E-4
28-29	0.0	2.5185299582412622E-5	0.0	0.005039578446440766	2.9466800511422766E-4
30-31	0.0	2.5185299582412622E-5	0.0	0.015783627248297988	3.0977918486367525E-4
32-33	0.0	2.5185299582412622E-5	0.0	0.04153055901139841	3.6770537390322426E-4
34-35	0.0	2.5185299582412622E-5	0.0	0.08139636972039935	3.878536135691544E-4
36-37	0.0	3.0222359498895147E-5	0.0	0.14376020854636948	4.080018532350845E-4
38-39	0.0	3.777794937361893E-5	0.0	0.23443987969284613	4.4829833256694466E-4
40-41	0.0	5.28891291230665E-5	0.0	0.3405430283035923	4.734836321493573E-4
42-43	0.0	7.051883883075534E-5	0.0	0.4422563791971239	4.8355775198232235E-4
44-45	0.0	7.051883883075534E-5	0.0	0.5658935333771458	5.037059916482525E-4
46-47	0.0	7.555589874723786E-5	0.0	0.7124744954767455	5.188171713977E-4
48-49	0.0	8.059295866372039E-5	0.0	0.8650722256465835	5.440024709801126E-4
50-51	0.0	8.059295866372039E-5	0.0	1.0249711741653629	5.440024709801126E-4
52-53	0.0	8.059295866372039E-5	0.0	1.1887511773497923	5.792618903954903E-4
54-55	0.0	8.059295866372039E-5	0.0	1.3758552864774938	6.24595429643833E-4
56-57	0.0	8.059295866372039E-5	0.0	1.5922524175494996	6.371880794350394E-4
58-59	5.0370599164825245E-6	8.311148862196166E-5	0.0	1.8297371999618592	6.422251393515218E-4
60-61	5.0370599164825245E-6	8.563001858020292E-5	0.0	2.073415047541534	6.497807292262457E-4
62-63	5.0370599164825245E-6	8.563001858020292E-5	0.0	2.3279376851213964	7.102254482240359E-4
64-65	5.0370599164825245E-6	8.563001858020292E-5	0.0	2.587311011400831	7.102254482240359E-4
66-67	5.0370599164825245E-6	8.563001858020292E-5	0.0	2.8808003444946015	7.429663376811724E-4
68-69	5.0370599164825245E-6	8.563001858020292E-5	0.0	3.210367619240179	7.757072271383087E-4
70-71	5.0370599164825245E-6	8.814854853844418E-5	0.0	3.570550144158136	7.908184068877563E-4
72-73	5.0370599164825245E-6	9.066707849668543E-5	0.0	3.9152688951324928	8.890410752591656E-4
74-75	5.0370599164825245E-6	9.066707849668543E-5	0.0	4.264876112755795	8.915596052174068E-4
76-77	5.0370599164825245E-6	9.066707849668543E-5	0.0	4.641005969369337	9.066707849668544E-4
78-79	5.0370599164825245E-6	9.066707849668543E-5	0.0	5.066912052077558	9.167449047998194E-4
80-81	5.0370599164825245E-6	9.066707849668543E-5	0.0	5.506954643441388	9.494857942569558E-4
82-83	5.0370599164825245E-6	9.066707849668543E-5	0.0	5.948604056918576	0.001007411983296505
84-85	5.0370599164825245E-6	9.066707849668543E-5	0.0	6.398572174847834	0.001007411983296505
86-87	1.7629709707688834E-5	9.066707849668543E-5	0.0	6.863533101618503	0.001009930513254746
88	2.0148239665930098E-5	9.066707849668543E-5	0.0	7.238723546557621	0.001027560222962435
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	32940	0.0	50.30662	2
GTACCTG	38155	0.0	43.89108	1
ACCTGGG	39040	0.0	41.756466	3
CCTGGGG	36065	0.0	32.653095	4
CTGGGGG	22615	0.0	24.1872	5
TGGGGGG	29990	0.0	23.880186	6
GTACATG	88155	0.0	22.959372	1
CATGGGG	54420	0.0	22.658676	4
TACATGG	90515	0.0	22.11849	2
ATGGGGG	32425	0.0	22.101364	5
ACATGGG	92610	0.0	21.002295	3
GTACCCG	4155	0.0	19.914776	1
TATAACG	1725	0.0	19.888414	2
GAGTACT	50805	0.0	18.356377	12-13
GTACACG	4240	0.0	17.852287	1
GTATCAA	118455	0.0	17.793	1
AGTACTT	54040	0.0	16.931318	12-13
TAACGCA	2335	0.0	16.703999	4
TATCACG	2160	0.0	16.53584	2
TACCCGG	4960	0.0	16.391958	2
>>END_MODULE