FastQCFastQC Report
Mon 6 Jun 2016
ERR522880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564979
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247690.25895509023072605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197060.20602240736754363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108060.11297463381780555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147200.047.5649071
GTATCAA259800.040.0081181
TCAACGC304650.033.4570354
CAACGCA309750.032.9809885
ATCAACG309600.032.8952063
TATCAAC321250.031.8668732
AACGCAG322200.031.8018426
ACGCAGA366950.027.8463137
CGCAGAG382300.026.7641228
GTACATG330750.026.1336981
TACATGG335600.025.0980952
ACATGGG338800.024.039743
GCAGAGT433350.023.1870969
CATGGGG229000.022.470244
TACCTGG73300.021.9304142
GAGTACT230800.021.08846512-13
CAGAGTA420950.019.84134310-11
AGAGTAC382150.019.7477110-11
ATGGGGG127100.019.336225
GTACTTT251600.019.14061414-15
Produced by FastQC (version 0.11.5)