FastQCFastQC Report
Mon 6 Jun 2016
ERR522880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564979
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225560.23581860451549344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188450.19702081938705773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103060.1077472308093933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145600.048.3761751
GTATCAA258100.039.5952381
TCAACGC306750.032.907594
ATCAACG313000.032.1619153
CAACGCA314100.032.07775
TATCAAC326700.030.9113252
AACGCAG328850.030.6955916
ACGCAGA379200.026.508177
GTACATG323400.025.998941
CGCAGAG393050.025.5189828
TACATGG334250.024.5419922
ACATGGG339500.023.4083393
GCAGAGT449850.022.1085459
CATGGGG223400.021.3725784
GAGTACT237100.020.32933412-13
AGAGTAC386500.019.84676410-11
CAGAGTA429350.019.28916510-11
GTACTTT257100.018.59994114-15
GTATAGG30400.018.2633111
TACCTGG73950.018.0004372
Produced by FastQC (version 0.11.5)