FastQCFastQC Report
Mon 6 Jun 2016
ERR522879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9349069
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT651340.6966896917757265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301470.32245991552741776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232940.2491584990976107No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT187460.2005119440235172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138630.14828214445737858No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA109880.1175304193390807No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG104760.11205393820496992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG335800.027.0090471
TACATGG346650.025.823342
ACATGGG348600.024.844313
CATGGGG226850.023.6879674
ATGGGGG131250.020.2025935
GAGTACT276550.019.58863812-13
TACCTGG63700.019.4112262
AGAGTAC428100.019.05691310-11
GTACTTT294950.018.2554714-15
AGTACTT292400.017.86800412-13
GTACCTG78350.016.622581
GAGTACA258300.016.291321
CATGGGA123050.016.0849914
GTATAGG26850.015.9350991
AGTACAT249250.015.6559552
TATACCG5800.015.4011435
GTATAAT47000.015.1055571
ACTTTTT364700.014.96213616-17
TACTTTT346900.014.89181814-15
ATGGGGA93650.014.8095415
Produced by FastQC (version 0.11.5)