FastQCFastQC Report
Mon 6 Jun 2016
ERR522879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9349069
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT669910.7165526321390933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278910.2983291705302421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230170.24619563723403903No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT187120.20014827144820518No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC157270.1682199585862507No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC137570.14714834172258223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131940.14112635172550336No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA112930.12079277626467406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG327950.027.0180131
TACATGG341900.025.3503552
ACATGGG345550.024.4805473
CATGGGG223700.022.289944
TACCTGG64550.020.242812
GAGTACT266450.020.12169512-13
AGAGTAC410300.019.76373710-11
ATGGGGG127150.019.2564495
GTACTTT284500.018.66873614-15
AGTACTT284100.018.43312312-13
GAGTACA255950.017.4010331
GTACCTG79500.017.0374871
GTATAGG27600.016.869661
AGTACAT248850.016.3380682
CATGGGA127300.016.169824
TACTTTT331300.015.51372514-15
ACTTTTT348350.015.31901516-17
TATAACG6900.014.9863492
GTATATA47400.014.5854261
GTATATG37950.014.1277571
Produced by FastQC (version 0.11.5)