FastQCFastQC Report
Mon 6 Jun 2016
ERR522878_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522878_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7682838
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349810.4553135182597889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289530.37685292856624075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174650.22732485053049406No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116570.1517277860082433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG111250.048.652462
GTACCTG128250.042.729851
ACCTGGG127350.041.5415843
GGTATCA266300.030.5765671
CCTGGGG115950.030.5359294
GTATCAA386050.028.4271971
CATGGGG206400.025.4238554
ATGGGGG136300.025.147895
TCAACGC442500.024.5462764
TGGGGGG152550.024.459076
ATCAACG444250.024.356713
TATCAAC444700.024.3214872
CAACGCA447200.024.2558065
GTACATG363250.023.7237131
AACGCAG459250.023.6678126
TACATGG367500.023.0582792
ACATGGG368850.022.2854333
CTGGGGG77400.021.9298745
GAGTACT278000.021.63564712-13
ACGCAGA507200.021.3839637
Produced by FastQC (version 0.11.5)