##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522878_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7682838
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5536364296631	33.0	31.0	34.0	28.0	34.0
2	31.894814129882732	34.0	31.0	34.0	28.0	35.0
3	31.891541641252882	34.0	31.0	34.0	28.0	36.0
4	34.19522785720589	35.0	34.0	37.0	32.0	37.0
5	34.2928665162535	35.0	34.0	37.0	31.0	37.0
6	34.52469582203868	35.0	35.0	37.0	31.0	37.0
7	34.41893763736786	36.0	34.0	37.0	31.0	37.0
8	34.53508612312273	36.0	34.0	37.0	31.0	37.0
9	35.72471917278485	37.0	35.0	39.0	31.0	39.0
10-11	35.700938702599224	37.0	34.5	39.0	31.5	39.0
12-13	35.732101275596335	37.5	35.0	39.0	31.0	39.0
14-15	36.522817349526306	38.0	36.0	41.0	31.0	41.0
16-17	36.680539469919836	38.0	36.5	41.0	31.0	41.0
18-19	36.55820824283943	38.0	35.5	41.0	30.5	41.0
20-21	36.66080932592878	38.5	35.5	41.0	31.0	41.0
22-23	36.511991987856575	38.0	35.5	41.0	31.0	41.0
24-25	36.49677365317348	38.0	35.0	41.0	30.5	41.0
26-27	36.3822152699302	38.0	36.5	40.5	30.0	41.0
28-29	36.15315492790555	38.0	35.0	40.0	29.5	41.0
30-31	35.96033939541613	37.5	35.5	40.0	29.0	41.0
32-33	35.92621658819306	37.5	35.0	40.0	29.0	41.0
34-35	35.775143963727984	37.5	35.0	40.0	28.5	41.0
36-37	35.60118188096638	37.5	34.5	40.0	28.0	41.0
38-39	35.432957521686646	37.0	35.0	40.0	28.0	41.0
40-41	35.38431423388076	37.0	34.5	40.0	27.5	41.0
42-43	35.16847133832576	36.0	35.0	40.0	27.0	41.0
44-45	35.096463312125024	37.0	34.0	40.0	27.0	41.0
46-47	34.86577902592766	36.5	34.0	40.0	25.0	41.0
48-49	34.724058804832275	37.0	34.0	40.0	24.0	41.0
50-51	33.677638849081546	36.5	32.5	38.5	23.0	40.0
52-53	33.881717276870866	36.5	33.0	38.5	23.5	39.5
54-55	34.736694304890975	37.5	34.0	40.0	24.5	41.0
56-57	34.87832575670605	37.0	34.5	40.0	25.5	41.0
58-59	34.813622973698	37.0	34.0	40.0	25.0	41.0
60-61	34.56530861382213	36.5	34.0	40.0	25.0	41.0
62-63	34.40315733586989	37.0	33.5	39.5	24.5	41.0
64-65	34.00606143198647	36.0	33.0	39.0	23.0	41.0
66-67	33.81565815913338	36.0	33.0	39.0	23.5	41.0
68-69	33.489695214190384	36.0	33.0	38.5	22.0	40.5
70-71	33.28377586511651	35.5	33.0	38.5	22.0	40.0
72-73	32.9550807917595	35.0	32.0	37.5	22.0	39.5
74-75	32.572513893954294	35.0	32.0	37.0	21.5	39.0
76-77	32.44119236927813	35.0	32.0	37.5	20.5	39.0
78-79	32.15774424763349	35.0	31.5	37.0	20.0	38.0
80-81	31.890651215084844	35.0	31.0	36.5	19.0	37.5
82-83	31.5639259216451	35.0	31.0	36.0	19.0	37.0
84-85	31.315826651557664	35.0	31.0	35.5	18.0	37.0
86-87	31.07063542144192	34.5	30.5	35.5	18.0	37.0
88-89	30.912438151110308	34.5	30.0	35.0	17.5	36.5
90-91	30.699254559317794	34.0	29.5	35.0	17.0	36.0
92-93	30.434147056074853	34.0	30.0	35.0	17.0	36.0
94-95	30.324377463119745	34.0	30.0	35.0	16.0	36.0
96-97	30.131537265265777	34.0	29.0	35.0	17.0	36.0
98-99	30.040518360532918	34.0	29.0	35.0	16.5	36.0
100	28.8263085854472	33.0	26.0	35.0	11.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06423343229888445
1101	2	0.05332311078018037
1101	3	0.11495995394106373
1101	4	0.04761147756163808
1101	5	0.05874679366534252
1101	6	0.07665939288303747
1101	7	0.13094532533164482
1101	8	0.10028337767466411
1101	9	0.20349161716845998
1101	10-11	0.06828890095471252
1101	12-13	0.022252963126369707
1101	14-15	-0.0398903408324216
1101	16-17	0.0454185067212407
1101	18-19	0.03544428648289255
1101	20-21	0.06560911324928043
1101	22-23	0.09484935041122355
1101	24-25	0.034923882514895865
1101	26-27	0.054105498359703574
1101	28-29	0.043850261460100626
1101	30-31	-0.05676356814394268
1101	32-33	-0.09481304515463052
1101	34-35	-0.10962771130330395
1101	36-37	-0.03503290743035592
1101	38-39	-0.10638499000852875
1101	40-41	-0.08964504729933509
1101	42-43	-0.08466085164152304
1101	44-45	-0.12218639368179396
1101	46-47	-0.06010667597004016
1101	48-49	-0.12217000786482402
1101	50-51	-0.2004924710830096
1101	52-53	-0.12319071188552044
1101	54-55	-0.025003814671968883
1101	56-57	-0.10357846363862677
1101	58-59	-0.052838523388189174
1101	60-61	-0.09911015352691521
1101	62-63	-0.2709190462363864
1101	64-65	-0.1866529101145602
1101	66-67	-0.1064770071156218
1101	68-69	-0.05735264166199272
1101	70-71	-0.24114909079013103
1101	72-73	-0.14377031632094628
1101	74-75	-0.2035288224860068
1101	76-77	-0.17609303396650233
1101	78-79	-0.29502347028331144
1101	80-81	-0.22815026051630838
1101	82-83	-0.4004427301608793
1101	84-85	-0.24353829655516535
1101	86-87	-0.279340661749913
1101	88-89	-0.40720244673857664
1101	90-91	-0.3478968221883818
1101	92-93	-0.43109627254639094
1101	94-95	-0.38073038006290005
1101	96-97	-0.35664979700082
1101	98-99	-0.3722448471616673
1101	100	-0.3285709958025258
1102	1	0.010302061911549032
1102	2	-0.04499165877261646
1102	3	0.01604032689172641
1102	4	0.03237293388054496
1102	5	0.07157849677027173
1102	6	0.05011750400827708
1102	7	0.09804629717397972
1102	8	0.17918455204382866
1102	9	0.059542004513836844
1102	10-11	0.0670664108931831
1102	12-13	0.019771673518128807
1102	14-15	-0.03171096401882778
1102	16-17	0.060080695895862846
1102	18-19	0.054261909252183216
1102	20-21	-0.010867468061071861
1102	22-23	-0.004814077370390635
1102	24-25	-0.1143987647904865
1102	26-27	-0.06490695967485749
1102	28-29	-0.040227158460552914
1102	30-31	-0.13196355322054387
1102	32-33	-0.11985330209175515
1102	34-35	-0.1382577271090284
1102	36-37	-0.550228793876002
1102	38-39	-0.25378872478431447
1102	40-41	-0.8756433247426969
1102	42-43	-0.8693599886127075
1102	44-45	-0.31530123297143575
1102	46-47	-0.2777395592260703
1102	48-49	-0.28202692356192216
1102	50-51	-0.4148317737180989
1102	52-53	-0.25199936646835397
1102	54-55	-0.12261721086512267
1102	56-57	-0.02156807631446611
1102	58-59	-0.09183641342428928
1102	60-61	-0.2573596473424544
1102	62-63	-0.40006636832565334
1102	64-65	-0.18774442257127077
1102	66-67	-0.19000158042391746
1102	68-69	-0.15498268522807024
1102	70-71	-0.22292420467389462
1102	72-73	-0.11923429000908214
1102	74-75	-0.2030953630876411
1102	76-77	-0.23563819378924222
1102	78-79	-0.2640734755259473
1102	80-81	-0.30147828696538426
1102	82-83	-0.3373434313662642
1102	84-85	-0.48823230228087056
1102	86-87	-0.4075230727527739
1102	88-89	-0.38107235711152754
1102	90-91	-0.34215121526477077
1102	92-93	-0.38316302223479326
1102	94-95	-0.5841291406071925
1102	96-97	-0.6431806666054776
1102	98-99	-0.5667107493988865
1102	100	-0.6610374044452243
1103	1	-0.007752122731584876
1103	2	-0.12943416292303667
1103	3	-0.06634735950767379
1103	4	0.03766990757264921
1103	5	0.015472527728206842
1103	6	0.11022701080158015
1103	7	0.08394781932884143
1103	8	0.02476579813582447
1103	9	0.09238499810535927
1103	10-11	0.054067653628308676
1103	12-13	0.04632563596474171
1103	14-15	-0.012006741937106824
1103	16-17	-0.01326363565786437
1103	18-19	0.02197014565536648
1103	20-21	-0.07903753405908986
1103	22-23	-0.03875405213303651
1103	24-25	-0.05733735071618895
1103	26-27	-0.1114494774788426
1103	28-29	-0.2719340364646641
1103	30-31	-0.2789402021491725
1103	32-33	-0.3665246156080002
1103	34-35	-0.399252628329414
1103	36-37	-0.47396412173109326
1103	38-39	-0.4058602398862945
1103	40-41	-0.35749652198102666
1103	42-43	-0.34589981918677637
1103	44-45	-0.24412073025941794
1103	46-47	-0.1475394432142707
1103	48-49	-0.2573798942554717
1103	50-51	-0.4305687660903672
1103	52-53	-0.2927155958586525
1103	54-55	-0.18150063639624392
1103	56-57	-0.07769552580280958
1103	58-59	-0.059909947862138324
1103	60-61	-0.057576495491353796
1103	62-63	-0.1347722036149861
1103	64-65	-0.19080447302857806
1103	66-67	-0.19303428027250646
1103	68-69	-0.2750854261156235
1103	70-71	-0.2700731754822314
1103	72-73	-0.2740900014162406
1103	74-75	-0.22100592442301092
1103	76-77	-0.15037017086864068
1103	78-79	-0.23802539063383676
1103	80-81	-0.10713703659469331
1103	82-83	-0.17387414796960599
1103	84-85	-0.2318438262359166
1103	86-87	-0.9163217505312957
1103	88-89	-0.368113343020525
1103	90-91	-0.6133302395655029
1103	92-93	-0.29889475320220527
1103	94-95	-0.6179941947040035
1103	96-97	-0.4380999757873667
1103	98-99	-0.4091341524755485
1103	100	-0.35633057775068266
1104	1	0.021153714174630522
1104	2	-0.04830978585111012
1104	3	-0.005268109051254299
1104	4	0.018088386903194476
1104	5	-0.035281279251549336
1104	6	0.012020290921618937
1104	7	0.07151461615973886
1104	8	-0.004648067963870517
1104	9	-0.027081083819744833
1104	10-11	-0.11513050058575658
1104	12-13	0.014087315114132082
1104	14-15	-0.20805591106812926
1104	16-17	-0.07080124271326582
1104	18-19	0.0017962533212738663
1104	20-21	-0.1357628126761412
1104	22-23	-0.09122358364670191
1104	24-25	-0.11361432472796906
1104	26-27	-0.032803424021629723
1104	28-29	-0.0860341602944672
1104	30-31	-0.11496233479822138
1104	32-33	-0.06868537470258218
1104	34-35	-0.1445296041921651
1104	36-37	-0.07263740910693883
1104	38-39	-0.06492650024476632
1104	40-41	-0.07865482113827937
1104	42-43	-0.3352760612638619
1104	44-45	-0.19864415827802162
1104	46-47	-0.09952216997489671
1104	48-49	-0.08619676126892273
1104	50-51	-0.21857381059691505
1104	52-53	-0.15779818299454007
1104	54-55	-5.811615530717518E-4
1104	56-57	-0.035692666673242
1104	58-59	-0.022242797681329307
1104	60-61	-0.09162210212473099
1104	62-63	-0.06382766048648847
1104	64-65	-0.10824596545225518
1104	66-67	-0.08131838915142708
1104	68-69	-0.18148576718744636
1104	70-71	-0.05965876211281085
1104	72-73	-0.02740918923587543
1104	74-75	-0.017999087561832994
1104	76-77	-0.11570874696782596
1104	78-79	-0.16920859765912155
1104	80-81	-0.21565797074154958
1104	82-83	-0.15184052574119988
1104	84-85	-0.08647473383371107
1104	86-87	-0.12556339016867923
1104	88-89	-0.08286266325229263
1104	90-91	-0.27892147288442004
1104	92-93	-0.21534011600131464
1104	94-95	-0.15173544039355846
1104	96-97	-0.28936822865189527
1104	98-99	-0.2561214850631437
1104	100	-0.22200183461391987
1105	1	0.07945112176812685
1105	2	0.035399209693231626
1105	3	0.05502174568119855
1105	4	0.08315467256586118
1105	5	0.16642845769079173
1105	6	0.13035287875130308
1105	7	0.09650047878370316
1105	8	-0.010864638825900386
1105	9	0.050640510585139964
1105	10-11	0.07475973771005329
1105	12-13	0.1618608185762227
1105	14-15	0.06908191017049603
1105	16-17	-0.013933071094328398
1105	18-19	0.056329982780184196
1105	20-21	0.0680589150342854
1105	22-23	0.080597535574924
1105	24-25	0.047254689206120304
1105	26-27	0.10080734081996212
1105	28-29	0.0258374018597749
1105	30-31	-0.05851795120976533
1105	32-33	0.0036769735900037404
1105	34-35	0.008605313732317654
1105	36-37	0.012014578789340646
1105	38-39	0.03933952062325119
1105	40-41	-0.01924361030260968
1105	42-43	-0.012618501880879762
1105	44-45	-0.019378168957715047
1105	46-47	-0.031137597728964295
1105	48-49	-0.03557894767213554
1105	50-51	-0.08141172374336492
1105	52-53	0.03882697736338514
1105	54-55	0.14633606578761515
1105	56-57	0.11602733171933721
1105	58-59	0.15018766346435086
1105	60-61	0.09022000263797025
1105	62-63	0.07884434615107239
1105	64-65	0.06035816645971437
1105	66-67	0.15162716853640035
1105	68-69	0.07136895013906042
1105	70-71	0.13777176257264045
1105	72-73	0.07949544710008638
1105	74-75	0.03736201853273968
1105	76-77	0.09965136701771371
1105	78-79	0.004916820101513508
1105	80-81	0.03177907943355862
1105	82-83	0.1057824378825778
1105	84-85	-7.187358174327585E-4
1105	86-87	0.045969880577541034
1105	88-89	0.023328665881798827
1105	90-91	0.055928559135182354
1105	92-93	0.05941683905716033
1105	94-95	0.1829548268799428
1105	96-97	0.05509757404100668
1105	98-99	0.0407928354442717
1105	100	0.007693837272196191
1106	1	-0.05183337597451754
1106	2	-0.06762753493569562
1106	3	-0.06247171921035033
1106	4	0.010186480275493182
1106	5	-0.015377515032341194
1106	6	0.055887225445168554
1106	7	0.06999622522521776
1106	8	-1.1018985134535484
1106	9	-0.005596625455531523
1106	10-11	0.01117998705529999
1106	12-13	-0.06692217798347144
1106	14-15	-0.06022561646309299
1106	16-17	-0.1207252571268782
1106	18-19	0.018595779629180242
1106	20-21	-0.06877360133294275
1106	22-23	-0.035313716597762834
1106	24-25	0.014358311771502485
1106	26-27	-0.054825010789343764
1106	28-29	-0.06788573037707835
1106	30-31	-0.040377597426839884
1106	32-33	-0.029255149988372864
1106	34-35	-0.08964059740509356
1106	36-37	0.006325436560317144
1106	38-39	0.009057884433076424
1106	40-41	-0.06170747025340262
1106	42-43	-0.052412334252089465
1106	44-45	-0.07794384447144154
1106	46-47	-0.07783268451843384
1106	48-49	-0.15584820530614962
1106	50-51	-0.06877263169851489
1106	52-53	-0.019699927929337946
1106	54-55	-0.006648512348959912
1106	56-57	-0.0624693700204233
1106	58-59	0.010308040348121494
1106	60-61	0.024703214201785784
1106	62-63	-0.014680437635149701
1106	64-65	-0.044051822113921446
1106	66-67	-0.06639809972251243
1106	68-69	-0.020487163335481284
1106	70-71	-0.051373955768220014
1106	72-73	-0.013000142399576475
1106	74-75	0.11116935348636048
1106	76-77	0.04315113625736444
1106	78-79	-0.09102094469908906
1106	80-81	-0.022156268586556394
1106	82-83	0.033345009917645996
1106	84-85	0.030014030664254676
1106	86-87	-0.018505307101342794
1106	88-89	-0.011405807730259454
1106	90-91	-0.026681476955243255
1106	92-93	0.008287369471002393
1106	94-95	-0.03137463129226248
1106	96-97	-0.00524571610540292
1106	98-99	-0.010083387058337223
1106	100	-0.14339836637753933
1107	1	-0.006089518240269598
1107	2	-0.11568166131515412
1107	3	-0.11743919822350435
1107	4	0.053098215636360635
1107	5	-0.041782166746216376
1107	6	-0.0549301216609166
1107	7	0.033630226092498106
1107	8	0.02445695984209806
1107	9	-0.025612221726149187
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2316	78-79	-0.1765962180775844
2316	80-81	0.09819411639003661
2316	82-83	0.10297560805589256
2316	84-85	0.27140815313674693
2316	86-87	0.32993880247542506
2316	88-89	0.22602236927895802
2316	90-91	0.39507941527313406
2316	92-93	0.4566080205206333
2316	94-95	0.44451884422455734
2316	96-97	0.4407363009006211
2316	98-99	0.4441671751913816
2316	100	0.20020292362871572
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	13859.0
1	1263.0
2	765.0
3	605.0
4	497.0
5	505.0
6	496.0
7	1132.0
8	2348.0
9	3765.0
10	5922.0
11	9127.0
12	13057.0
13	16844.0
14	20272.0
15	23488.0
16	28217.0
17	35277.0
18	43427.0
19	52619.0
20	62698.0
21	74410.0
22	85102.0
23	91279.0
24	94193.0
25	97620.0
26	104110.0
27	115694.0
28	133161.0
29	157766.0
30	192345.0
31	239097.0
32	307324.0
33	414247.0
34	604996.0
35	990062.0
36	1133882.0
37	1101986.0
38	1132194.0
39	276802.0
40	385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25683076848076	17.750202334000413	12.772271045831829	23.220695851686994
2	16.16603288541841	20.30446769539215	40.512454928240786	23.017044490948653
3	19.308617357449563	26.326102346608437	29.737029230957653	24.62825106498435
4	13.004284005920914	16.477082441947353	37.22030935559991	33.29832419653182
5	13.23721711331971	36.83684750183872	34.01676465131267	15.9091707335289
6	30.08802646653466	35.296495058143826	19.704653998292272	14.910824477029246
7	27.39770183523533	32.586431742345376	22.192267683433162	17.823598738986128
8	26.264032626999644	34.2330196469828	21.153575116118585	18.34937260989897
9	27.760435210698486	16.017749542413778	20.42033979888133	35.80147544800641
10-11	25.027179933771254	25.737009413939038	28.59727010643104	20.638540545858667
12-13	25.64939422627145	23.758012315984335	28.33838479174226	22.254208666001954
14-15	23.264151445555466	25.058440873934707	26.80301739905952	24.87439028145031
16-17	22.520883312959846	27.543923031929683	27.415301943159605	22.51989171195087
18-19	22.08770753107991	26.754072326449013	29.391483575635068	21.766736566836002
20-21	23.198438258491187	26.110692083244164	29.189161503489387	21.501708154775265
22-23	22.776496601497605	25.851347944745047	29.12205746269163	22.250097991065722
24-25	22.36774005696451	25.763813685065735	29.281163773848924	22.587282484120834
26-27	22.147335054051393	25.954252507666148	29.31215140405231	22.586261034230144
28-29	22.191554836044244	26.610490151275716	29.110109763172847	22.087845249507193
30-31	23.168376230243513	26.02458507058486	29.205044618966763	21.601994080204868
32-33	22.68692780423432	26.07792631122987	29.206273998384404	22.028871886151414
34-35	22.72710252426597	26.443322090367143	29.007231125150756	21.82234426021613
36-37	22.664963816610985	26.471794023340774	28.83458981155306	22.028652348495186
38-39	22.641526051921037	26.692893063551004	28.625393027342323	22.040187857185636
40-41	22.7706016164927	26.350699804597344	28.77252862884445	22.106169950065507
42-43	22.542069313599686	26.509441569839588	28.37418325599433	22.574305860566398
44-45	22.35049784149309	26.6905716517441	28.611198560064732	22.347731946698076
46-47	22.254910857075085	26.383327134901897	28.402604528484197	22.959157479538817
48-49	22.41247105627259	26.43487849718289	28.41416590882831	22.73848453771621
50-51	22.401716678727944	26.822733271884207	28.425840495348943	22.349709554038906
52-53	22.375050836239417	26.9249668524556	28.048861251925057	22.651121059379925
54-55	22.13501219274256	26.774381733007324	28.28485926958392	22.805746804666196
56-57	22.413394507014434	26.84460827491642	27.842772947030753	22.899224271038392
58-59	22.287260333367804	26.858172685581067	28.18263943966073	22.671927541390403
60-61	22.82124115566711	26.850702161273553	28.199872904252643	22.12818377880669
62-63	22.554725258662515	26.821864719284573	28.264116850756356	22.35929317129656
64-65	22.45284710466189	26.569897002792214	28.143924386237178	22.833331506308717
66-67	22.398891608128764	26.62090956833691	28.005688130100314	22.97451069343401
68-69	22.110517019196514	26.7085613384838	28.25460119956927	22.92632044275042
70-71	22.32407464701617	26.59152436743058	28.209523011341904	22.874877974211337
72-73	22.160574461533702	27.12372147443804	27.7035331880551	23.012170875973155
74-75	22.3541990119373	27.033644476644415	27.606945873098613	23.005210638319674
76-77	22.15778451588087	26.709743624683956	27.825981515530213	23.306490343904958
78-79	22.136059021955333	26.743972736326704	27.918593232883342	23.201375008834617
80-81	22.23452273715675	26.915764056751296	27.558047993976036	23.291665212115912
82-83	22.46149302185929	27.011668986916483	27.558564038470664	22.968273952753567
84-85	22.56802626631684	27.17540697499493	27.24163143604092	23.014935322647307
86-87	22.202632580690402	27.18739727647279	27.430426009660717	23.17954413317609
88-89	22.16549796761233	26.80343404334194	27.625180460883392	23.40588752816234
90-91	22.897609151849156	26.622834396182682	27.5404992754264	22.939057176541763
92-93	22.766221192618012	26.842394214322656	27.408248784439042	22.983135808620286
94-95	22.4970333283364	26.884574788808806	27.36071718826551	23.257674694589287
96-97	22.397189455838852	26.71596291791048	27.52523695138293	23.36161067486774
98-99	22.505443680339	26.67296352195841	27.242166614138497	23.5794261835641
100	22.512966603897176	26.854817634737945	27.277800302160315	23.35441545920457
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7125.0
1	7884.0
2	9898.0
3	8559.0
4	4551.0
5	3392.0
6	4528.0
7	7485.5
8	11619.5
9	13936.5
10	11859.5
11	9335.5
12	9444.0
13	10099.0
14	10783.5
15	11479.0
16	12131.5
17	12892.5
18	13805.0
19	14598.0
20	15766.0
21	17423.0
22	19518.0
23	22872.0
24	27150.0
25	32191.0
26	39147.5
27	48121.0
28	57173.5
29	66641.0
30	78081.0
31	89866.5
32	102542.5
33	116898.0
34	131388.5
35	145501.5
36	163375.5
37	180290.5
38	191057.0
39	201186.5
40	211137.0
41	220551.5
42	228575.0
43	238458.0
44	250806.0
45	262327.0
46	273951.5
47	288220.0
48	307186.0
49	322956.5
50	334490.5
51	329301.0
52	308481.0
53	291635.0
54	275444.0
55	253464.0
56	227102.5
57	203121.0
58	180312.5
59	152323.5
60	123694.5
61	101056.0
62	82215.5
63	64294.0
64	48682.0
65	37264.5
66	28335.5
67	21780.5
68	16872.0
69	13207.0
70	10597.5
71	8363.5
72	6778.5
73	5588.0
74	4463.5
75	3408.5
76	2471.5
77	1697.5
78	1171.5
79	825.5
80	591.0
81	415.5
82	293.5
83	207.5
84	149.0
85	115.5
86	89.5
87	64.5
88	48.0
89	42.5
90	33.5
91	33.0
92	30.0
93	19.0
94	15.0
95	16.5
96	13.0
97	6.5
98	4.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14469913331505885
2	0.07335830847923645
3	0.31290520508176795
4	0.3108877214383539
5	0.3121893237889436
6	0.2606979347996144
7	0.2618173128211216
8	0.26008618169483727
9	0.2719567951322155
10-11	0.24855398486861238
12-13	0.2586218790504238
14-15	0.23202363501612297
16-17	0.24034087403639123
18-19	0.2541769070231599
20-21	0.2550880286685727
22-23	0.2456644276503032
24-25	0.24656904128396306
26-27	0.24456457366405487
28-29	0.2552702529976553
30-31	0.24147977609315724
32-33	0.2529208607548408
34-35	0.2368135316662931
36-37	0.24501362647500832
38-39	0.2464063409901393
40-41	0.2629106587956169
42-43	0.24959526674908414
44-45	0.23492620825793803
46-47	0.23773116132345887
48-49	0.24788365965805864
50-51	0.2408419909413683
52-53	0.25708598827672796
54-55	0.2667373697063507
56-57	0.2625917662197224
58-59	0.26006665765957837
60-61	0.24128453574056877
62-63	0.2679413518806462
64-65	0.2617001686095685
66-67	0.2676875394222812
68-69	0.27339506572961714
70-71	0.2937976825751109
72-73	0.3030455672760508
74-75	0.28224596171362715
76-77	0.278978939813647
78-79	0.2761284306658555
80-81	0.26169366059781557
82-83	0.27265315238978094
84-85	0.28337835575864023
86-87	0.2758420781487258
88-89	0.2850248827321362
90-91	0.2790049718606588
92-93	0.25921410811994217
94-95	0.27951259677738877
96-97	0.2785884591084701
98-99	0.26400400477011227
100	0.28640458122376133
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7682838.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.54848851587197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.17305709916103	42.109988361423135
2	16.073038512531653	18.499581445071918
3	5.2538774158938555	9.070581123971001
4	2.1343676057582885	4.913185185945201
5	1.0434549358255794	3.0024627195594142
6	0.5792349331775508	2.0000456939976092
7	0.3780141394844254	1.5227899655469679
8	0.24845912300335057	1.1438757589457555
9	0.17899307798474007	0.9270702983542853
>10	0.8625219839180704	8.655868311184369
>50	0.046997289017290084	1.8549443701725437
>100	0.024827212308046732	2.6542301658492784
>500	0.002056190775452088	0.8159462875935086
>1k	8.977609469537572E-4	0.9792143464921176
>5k	8.688009160030425E-5	0.29933910088909754
>10k+	1.15840122133739E-4	1.5508768650039453
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34981	0.4553135182597889	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28953	0.37685292856624075	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17465	0.22732485053049406	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11657	0.1517277860082433	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.301602350589717E-5	0.0
8	0.0	0.0	0.0	1.301602350589717E-5	0.0
9	0.0	0.0	0.0	1.301602350589717E-5	0.0
10-11	0.0	0.0	0.0	2.603204701179434E-5	0.0
12-13	0.0	0.0	0.0	2.603204701179434E-5	6.508011752948585E-6
14-15	0.0	0.0	0.0	3.904807051769151E-5	2.603204701179434E-5
16-17	1.301602350589717E-5	0.0	0.0	5.206409402358868E-5	3.904807051769151E-5
18-19	1.301602350589717E-5	0.0	0.0	6.508011752948585E-5	6.508011752948585E-5
20-21	1.301602350589717E-5	0.0	0.0	1.5619228207076603E-4	7.158812928243443E-5
22-23	1.301602350589717E-5	0.0	0.0	3.384166111533264E-4	9.762017629422877E-5
24-25	1.301602350589717E-5	6.508011752948585E-6	0.0	9.176296571657505E-4	1.366682468119203E-4
26-27	1.301602350589717E-5	1.301602350589717E-5	0.0	0.0019524035258845754	1.692083055766632E-4
28-29	2.603204701179434E-5	1.301602350589717E-5	0.0	0.0052910135551472	1.692083055766632E-4
30-31	3.904807051769151E-5	1.301602350589717E-5	0.0	0.015137635337358409	1.7571631732961178E-4
32-33	3.904807051769151E-5	1.301602350589717E-5	0.0	0.039256326893785866	1.9524035258845754E-4
34-35	3.904807051769151E-5	1.301602350589717E-5	0.0	0.07669691850849908	1.9524035258845754E-4
36-37	3.904807051769151E-5	1.301602350589717E-5	0.0	0.13584172931929583	1.9524035258845754E-4
38-39	3.904807051769151E-5	1.301602350589717E-5	0.0	0.22205986902235866	1.9524035258845754E-4
40-41	3.904807051769151E-5	1.301602350589717E-5	0.0	0.3260513888227241	2.0825637609435472E-4
42-43	3.904807051769151E-5	1.301602350589717E-5	0.0	0.42463475085638924	2.0825637609435472E-4
44-45	3.904807051769151E-5	1.301602350589717E-5	0.0	0.5400152912244147	2.0825637609435472E-4
46-47	3.904807051769151E-5	1.301602350589717E-5	0.0	0.6741779535114498	2.2778041135320046E-4
48-49	3.904807051769151E-5	1.301602350589717E-5	0.0	0.8203283734474163	2.603204701179434E-4
50-51	3.904807051769151E-5	1.301602350589717E-5	0.0	0.9757592181430872	2.603204701179434E-4
52-53	3.904807051769151E-5	1.301602350589717E-5	0.0	1.1363574241705994	2.9286052888268633E-4
54-55	3.904807051769151E-5	1.301602350589717E-5	0.0	1.3231829175624945	3.384166111533264E-4
56-57	3.904807051769151E-5	1.301602350589717E-5	0.0	1.5373680923637854	3.44924622906275E-4
58-59	4.55560822706401E-5	1.301602350589717E-5	0.0	1.787041455253905	3.514326346592236E-4
60-61	5.206409402358868E-5	1.301602350589717E-5	0.0	2.0315149167534186	3.514326346592236E-4
62-63	5.206409402358868E-5	1.301602350589717E-5	0.0	2.2849368944132364	3.7095666991806935E-4
64-65	5.206409402358868E-5	1.301602350589717E-5	0.0	2.5406041361278215	3.774646816710179E-4
66-67	5.206409402358868E-5	1.301602350589717E-5	0.0	2.835996281582405	3.8397269342396647E-4
68-69	5.206409402358868E-5	1.301602350589717E-5	0.0	3.167625036477406	3.904807051769151E-4
70-71	5.206409402358868E-5	1.301602350589717E-5	0.0	3.5247651974439655	3.904807051769151E-4
72-73	5.206409402358868E-5	1.301602350589717E-5	0.0	3.858782392652299	4.4254479920050375E-4
74-75	5.206409402358868E-5	1.301602350589717E-5	0.0	4.207943731209744	4.4254479920050375E-4
76-77	5.206409402358868E-5	1.301602350589717E-5	0.0	4.5865733469845384	4.490528109534523E-4
78-79	5.206409402358868E-5	1.301602350589717E-5	0.0	5.0125812883208	4.555608227064009E-4
80-81	5.206409402358868E-5	1.9524035258845754E-5	0.0	5.439090346562038	5.076249167299896E-4
82-83	5.206409402358868E-5	3.904807051769151E-5	0.0	5.882272930914332	5.987370812712698E-4
84-85	5.206409402358868E-5	3.904807051769151E-5	0.0	6.336096114482695	6.052450930242185E-4
86-87	5.206409402358868E-5	3.904807051769151E-5	0.0	6.80901380453421	6.11753104777167E-4
88	5.206409402358868E-5	3.904807051769151E-5	0.0	7.192589509241246	6.247691282830641E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	11125	0.0	48.65246	2
GTACCTG	12825	0.0	42.72985	1
ACCTGGG	12735	0.0	41.541584	3
GGTATCA	26630	0.0	30.576567	1
CCTGGGG	11595	0.0	30.535929	4
GTATCAA	38605	0.0	28.427197	1
CATGGGG	20640	0.0	25.423855	4
ATGGGGG	13630	0.0	25.14789	5
TCAACGC	44250	0.0	24.546276	4
TGGGGGG	15255	0.0	24.45907	6
ATCAACG	44425	0.0	24.35671	3
TATCAAC	44470	0.0	24.321487	2
CAACGCA	44720	0.0	24.255806	5
GTACATG	36325	0.0	23.723713	1
AACGCAG	45925	0.0	23.667812	6
TACATGG	36750	0.0	23.058279	2
ACATGGG	36885	0.0	22.285433	3
CTGGGGG	7740	0.0	21.929874	5
GAGTACT	27800	0.0	21.635647	12-13
ACGCAGA	50720	0.0	21.383963	7
>>END_MODULE