FastQCFastQC Report
Mon 6 Jun 2016
ERR522878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7682838
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304290.396064579260945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271390.3532418619265433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163650.21300722467400718No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99110.12900180896694685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG107800.050.66062
GTACCTG127650.043.1730881
ACCTGGG124150.042.8856933
CCTGGGG112850.030.689884
GGTATCA268150.029.214841
TAACGCA9700.026.6452714
GTATCAA372250.026.3503231
TATCACG6750.024.3695342
ATAACGC9700.024.2229753
TATAACG9100.023.7574252
TGGGGGG142200.023.0334826
CATGGGG204200.022.7828834
CTGGGGG72950.022.481715
ATGGGGG135750.022.3279635
TCAACGC434350.022.2764384
ATCAACG437450.022.1185763
CAACGCA439750.022.034955
GTACATG361550.021.9798491
TATCAAC442850.021.9152472
AACGCAG453950.021.4697576
Produced by FastQC (version 0.11.5)