FastQCFastQC Report
Mon 6 Jun 2016
ERR522877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10972191
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT573430.5226212339905494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344170.31367481663416175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284400.25920073757374434No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT156530.14266065911539455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155040.14130268056762774No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC123090.11218361036551404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG390050.023.414971
TACATGG397600.022.5440142
ACATGGG392400.022.0277063
GAGTACT319150.020.85941112-13
CATGGGG228350.020.3678914
TACCTGG92350.019.7073782
GTACTTT339050.019.49701914-15
CGCCGTA41500.018.23038194
AGTACTT335550.018.06811512-13
AGAGTAC526600.017.99636710-11
CCGTATC32800.017.4784994
TAACGCA11350.016.9878644
TATAACG9500.016.8310052
CGTATCA24800.016.10590794
ATGGGGG124350.016.0718615
ACTTTTT418350.016.06660716-17
ACCTGGG108700.015.7479713
GAGTACA324850.015.7076621
GTATAGG30350.015.3405581
TACTTTT395150.015.23034814-15
Produced by FastQC (version 0.11.5)