FastQCFastQC Report
Mon 6 Jun 2016
ERR522877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10972191
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT581290.5297847986787689No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322610.29402514046647565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282210.2572047825270268No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT156680.14279736836516974No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT154470.14078318541848206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152810.13927026972097004No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC130280.11873654040473774No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC116480.10615928942542105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG396150.022.6532251
TACATGG402800.021.8214842
ACATGGG400250.021.0433583
GAGTACT317600.020.0360412-13
TACCTGG94350.019.628472
CATGGGG227700.019.093484
GTACTTT335250.018.80566614-15
TATAACG9750.018.3193952
TATACCG7900.017.2538995
AGTACTT337350.017.2400412-13
AGAGTAC528400.017.152910-11
ACCTGGG109900.016.0376953
CATGGGT81600.015.8973714
ACTTTTT404650.015.661818516-17
ATAACGC12850.015.3622413
GAGTACA331750.015.3542591
ATGGGGG118500.014.9137135
TAACGCA11050.014.8871724
TACTTTT390250.014.78241514-15
GTATAGC34300.014.6723951
Produced by FastQC (version 0.11.5)