FastQCFastQC Report
Mon 6 Jun 2016
ERR522876_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522876_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40100379
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT44550.012.1271611
CGACGAT76900.011.7296191
CGACAAT48250.011.0998351
CCGGAAT99000.010.9144441
CGCGCTA21200.010.8590289
CCGGGAT115550.010.8555121
TTGCGCG29100.010.8167238
CTCGAAT129150.010.4762791
CGGAAAT96600.09.9697841
TCGAATG85700.09.4836592
CGCGAAT28550.09.3794571
CTGGAAT398850.09.3052171
CGAAGAT162300.09.2917071
CTCGGAT162300.09.1469761
CGAAAAT76500.09.0274461
CGCAAAT52600.08.9314761
CGGAGAT143100.08.9297291
CGGGCAT127150.08.9045021
CGCAGAT106400.08.8307451
CCGGTAT61050.08.7725971
Produced by FastQC (version 0.11.5)