FastQCFastQC Report
Mon 6 Jun 2016
ERR522876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40100379
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA22300.016.0204129
CGACGAT78050.012.1725891
CCGGGAT115900.011.2003131
TTGCGCG33500.010.3836938
CGCGAAT27850.010.1328251
CTCGAAT133600.010.0684831
CCGAAAT72800.09.8847241
CGCGGAT46100.09.5902821
CGCATAT23100.09.5695251
CCGGTTT87100.09.5578371
CGGAAAT92850.09.472491
CCGGAAT98100.09.4449951
TCGAATG87150.09.38170052
CGCGGGT75350.09.3005261
CGGGGAT137450.09.2731881
CGGCGAT54100.09.2153761
TGCGCGG71950.09.0158627
CGACAAT52050.08.8554331
CTCGTAT74050.08.8286491
CTGGAAT397400.08.7225631
Produced by FastQC (version 0.11.5)