FastQCFastQC Report
Mon 6 Jun 2016
ERR522875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4882346
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138900.2844943803655046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116650.23892202641926646No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73140.14980503225293743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61830.1266399390784676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG50150.033.8538362
ACCTGGG59600.027.3027173
GTATCAA233950.023.679691
GTACATG158550.023.639911
GGTATCA174500.023.6351261
GTACCTG75050.022.8089071
TACATGG163700.022.552392
ATAACGC5450.022.4363843
CCGTATC24800.022.17031594
TATAACG5150.021.9167212
ACATGGG165300.021.3669783
TCAACGC258250.021.234124
CAACGCA258200.021.1456415
CGTATCA18050.021.08849194
CATGGGG106250.021.069494
GAGTACT129700.020.9269212-13
ATCAACG262600.020.8644643
AACGCAG264700.020.7411776
TATCAAC269050.020.4165062
CGCCGTA38550.019.62633394
Produced by FastQC (version 0.11.5)