FastQCFastQC Report
Mon 6 Jun 2016
ERR522875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4882346
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT187130.38327885815548507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129260.2647497739816064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116580.2387786527214581No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT97720.20014968214051193TruSeq Adapter, Index 10 (95% over 21bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71580.1466098469874933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59810.12250258379885408No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT51400.10527725810501753RNA PCR Primer, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG53400.031.2474692
ACCTGGG62250.026.3514753
GGTATCA177200.023.8397031
GTACATG163100.022.7279621
GTACCTG77550.022.2017651
GTATCAA232100.022.1733061
TACATGG163950.021.759962
TATAACG3500.021.4871942
ACATGGG164800.020.8487033
GAGTACT124250.020.10394312-13
TCAACGC256700.019.591914
CAACGCA258800.019.4878185
ATCAACG260800.019.3379763
CATGGGG105550.019.237314
AACGCAG267050.018.9888556
TATCAAC266250.018.9778352
GTACTTT132200.018.66370214-15
CCTGGGG58550.018.3834694
ATAACGC5100.017.510653
AGAGTAC211650.017.4868310-11
Produced by FastQC (version 0.11.5)