FastQCFastQC Report
Mon 6 Jun 2016
ERR522874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15265724
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464240.304106113801088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346800.2271755994016399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238840.15645507543566228No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT196390.12864768156426776No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157780.103355726855798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG275700.054.7830852
GTACCTG321850.047.5524751
ACCTGGG316450.047.416493
CCTGGGG284400.035.3279464
GTACCCG30500.027.7575911
TATCACG13300.026.1630152
TATAACG15950.025.353942
CTGGGGG183350.025.2051285
GTACATG659650.024.8198721
CATGGGG385700.024.7938964
TGGGGGG249650.023.7364486
TACATGG680650.023.7030682
ATGGGGG238600.023.4670035
ACATGGG684750.022.9293693
ATAACGC17800.022.718843
TAACGCA18500.021.3476964
TACCCGG36700.021.2691292
GTATCAA782050.020.6826761
GTACACG32350.020.5000021
GAGTACT415300.018.51712-13
Produced by FastQC (version 0.11.5)