##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522874_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15265724
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.356900989432273	33.0	31.0	34.0	28.0	34.0
2	31.69249896041616	34.0	31.0	34.0	27.0	35.0
3	31.68335605962744	34.0	31.0	34.0	27.0	36.0
4	33.97762104175341	35.0	34.0	37.0	31.0	37.0
5	34.067312431431354	35.0	34.0	37.0	31.0	37.0
6	34.290376597926176	35.0	35.0	37.0	30.0	37.0
7	34.17198765024181	35.0	34.0	37.0	31.0	37.0
8	34.27549515502835	36.0	34.0	37.0	30.0	37.0
9	35.44923332820638	37.0	35.0	39.0	30.0	39.0
10-11	35.410413977090116	37.0	34.5	39.0	30.0	39.0
12-13	35.43913544486983	37.0	35.0	39.0	30.0	39.0
14-15	36.21471762492234	38.0	35.0	41.0	30.0	41.0
16-17	36.37329428332387	38.0	36.0	41.0	30.0	41.0
18-19	36.22653904262909	38.0	35.5	40.5	29.5	41.0
20-21	36.32115954670738	38.0	35.0	40.0	30.0	41.0
22-23	36.16798987064092	38.0	35.0	40.0	29.5	41.0
24-25	36.14196450820151	38.0	35.0	40.0	29.5	41.0
26-27	36.02639589841923	38.0	36.0	40.0	29.5	41.0
28-29	35.79614448682552	38.0	35.0	40.0	29.0	41.0
30-31	35.617169876777545	37.0	35.0	40.0	28.0	41.0
32-33	35.59444606099258	37.5	34.5	40.0	27.0	41.0
34-35	35.44411604716554	37.5	34.5	40.0	27.0	41.0
36-37	35.264338429019155	37.5	34.5	40.0	27.0	41.0
38-39	35.09906087651002	36.5	34.5	40.0	26.5	41.0
40-41	35.04918253467703	37.0	34.0	40.0	26.0	41.0
42-43	34.844025379995074	36.0	34.5	40.0	25.5	41.0
44-45	34.77016330833703	37.0	33.5	40.0	25.0	41.0
46-47	34.5630155503925	36.5	33.5	40.0	24.0	41.0
48-49	34.43440229235115	37.0	33.0	40.0	23.5	41.0
50-51	33.40965400658364	36.5	32.0	38.5	23.0	40.0
52-53	33.62886221446163	36.5	32.5	38.5	23.0	39.5
54-55	34.4843200361804	37.0	33.0	39.5	24.0	41.0
56-57	34.6207706231293	37.0	34.0	40.0	24.5	41.0
58-59	34.54556377411251	37.0	33.5	40.0	24.0	41.0
60-61	34.28849319560605	36.5	34.0	39.5	24.0	41.0
62-63	34.114520673896635	36.5	33.0	39.0	23.5	41.0
64-65	33.71163378821731	36.0	33.0	39.0	23.0	41.0
66-67	33.51154471939883	36.0	33.0	38.5	22.5	40.5
68-69	33.17326859178117	35.0	32.5	37.5	22.0	40.0
70-71	32.96118972149634	35.0	32.0	37.5	21.5	40.0
72-73	32.63266619388638	35.0	32.0	37.5	21.0	39.0
74-75	32.25540550844493	35.0	32.0	36.5	20.5	39.0
76-77	32.13418017383257	35.0	31.0	37.0	19.5	38.5
78-79	31.855678119164214	35.0	31.0	36.5	20.0	38.0
80-81	31.58829473138647	35.0	30.5	36.0	18.5	37.5
82-83	31.27327521446084	34.5	30.5	35.5	18.0	37.0
84-85	31.03586036928219	35.0	30.0	35.0	18.0	37.0
86-87	30.794682027527813	34.0	29.5	35.0	17.5	37.0
88-89	30.632140997701782	34.0	29.5	35.0	17.5	36.5
90-91	30.415532568255525	34.0	29.0	35.0	17.0	36.0
92-93	30.157125236903276	34.0	29.0	35.0	17.0	36.0
94-95	30.054110633730836	34.0	29.0	35.0	16.0	36.0
96-97	29.857651363276318	34.0	29.0	35.0	17.0	36.0
98-99	29.76700060213325	34.0	28.5	35.0	15.5	36.0
100	28.511904577863454	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1464329927193404
1101	2	0.09157545768140096
1101	3	0.1667140125931077
1101	4	0.1640161808652607
1101	5	0.0950262214091353
1101	6	0.2168324005131339
1101	7	0.15708220579934107
1101	8	0.15212459065875095
1101	9	0.2371274825496812
1101	10-11	0.18777678582482338
1101	12-13	0.116078833353221
1101	14-15	0.06653578244685576
1101	16-17	0.1471028863732542
1101	18-19	0.15119979337264766
1101	20-21	0.21110203899146285
1101	22-23	0.15819353281409576
1101	24-25	0.057699223374214625
1101	26-27	0.07506159592988837
1101	28-29	0.01763167371341723
1101	30-31	-0.0128837209298851
1101	32-33	-0.07580890885486014
1101	34-35	-0.03135508554403543
1101	36-37	-0.059775577817916314
1101	38-39	-0.014792899961570072
1101	40-41	-0.03951082504836023
1101	42-43	-0.041298216322978476
1101	44-45	-0.023672077934918434
1101	46-47	0.00979908757542347
1101	48-49	-0.04246551912761021
1101	50-51	-0.15531678198725274
1101	52-53	-0.07075550997244306
1101	54-55	-0.02718391604837933
1101	56-57	-0.004696045165445639
1101	58-59	0.018316761363301737
1101	60-61	0.05750904265179457
1101	62-63	-0.06324097870074752
1101	64-65	-0.07453363619146813
1101	66-67	-0.03576727015205705
1101	68-69	-0.02683430849602786
1101	70-71	-0.20814204886489307
1101	72-73	-0.07262917289062898
1101	74-75	-0.09490955507735777
1101	76-77	-0.022950717010196797
1101	78-79	-0.20753246329810437
1101	80-81	-0.20253066592300684
1101	82-83	-0.3540490819831028
1101	84-85	-0.34556708318417506
1101	86-87	-0.2139466438498836
1101	88-89	-0.32504371992466474
1101	90-91	-0.28685750240007124
1101	92-93	-0.2497458845849998
1101	94-95	-0.19632142958079513
1101	96-97	-0.25128944112133667
1101	98-99	-0.28605243603467656
1101	100	-0.25079650453341173
1102	1	-0.009629915923895283
1102	2	-0.030212129322158887
1102	3	-0.040274394301292205
1102	4	8.331817522062579E-4
1102	5	-0.08043068269899578
1102	6	0.03657938672971284
1102	7	0.03387596316279229
1102	8	0.019602927519294155
1102	9	0.07995364903705138
1102	10-11	0.048939828650333084
1102	12-13	-0.06314227824602625
1102	14-15	-0.07651165173910357
1102	16-17	-2.8040590562739E-4
1102	18-19	0.05093117336942754
1102	20-21	-0.07078457240739766
1102	22-23	-0.05760388199297495
1102	24-25	-0.09361914968689433
1102	26-27	-0.042791365445758345
1102	28-29	-0.0623042809651011
1102	30-31	-0.1462706068843218
1102	32-33	-0.09918930839582174
1102	34-35	-0.18699894568179332
1102	36-37	-0.5588169392234974
1102	38-39	-0.2796003401927223
1102	40-41	-0.8550423089782271
1102	42-43	-0.9044934999581997
1102	44-45	-0.38673900573012077
1102	46-47	-0.29575063457377126
1102	48-49	-0.4460727587564648
1102	50-51	-0.4572013809538049
1102	52-53	-0.32178704524650215
1102	54-55	-0.14524092821386603
1102	56-57	-0.04958529845645643
1102	58-59	-0.09413740564182405
1102	60-61	-0.2372655907063077
1102	62-63	-0.44246444967135545
1102	64-65	-0.19366513301428512
1102	66-67	-0.1348601011510766
1102	68-69	-0.09370920129673266
1102	70-71	-0.18884555163499073
1102	72-73	-0.10958644026300135
1102	74-75	-0.19384942171436137
1102	76-77	-0.33281073429955654
1102	78-79	-0.22954315719526264
1102	80-81	-0.3319997298038686
1102	82-83	-0.412296970543121
1102	84-85	-0.5535677997271158
1102	86-87	-0.43681585168026515
1102	88-89	-0.4481357165050639
1102	90-91	-0.45319708495575384
1102	92-93	-0.47880206079920384
1102	94-95	-0.6141494550020035
1102	96-97	-0.8057087968095296
1102	98-99	-0.5806874461611642
1102	100	-0.6271530861740011
1103	1	0.05434581376793801
1103	2	0.046595280628217495
1103	3	0.023444809384724152
1103	4	0.03910675263424679
1103	5	0.13690215570841247
1103	6	0.16599784618934876
1103	7	0.11457595006142895
1103	8	0.05442163991860838
1103	9	0.055167517218251305
1103	10-11	0.04830255911021197
1103	12-13	0.10259850126622183
1103	14-15	0.0715007483538912
1103	16-17	-0.022674918107128406
1103	18-19	0.008491284103250507
1103	20-21	-0.0770142063949919
1103	22-23	-0.05143930921938278
1103	24-25	-0.09391715230412956
1103	26-27	-0.09090394539018831
1103	28-29	-0.2540046925032371
1103	30-31	-0.23014931947382422
1103	32-33	-0.2528054021310808
1103	34-35	-0.26323941062224776
1103	36-37	-0.41295377090516894
1103	38-39	-0.2983073068085602
1103	40-41	-0.19414443594869368
1103	42-43	-0.22818584328054214
1103	44-45	-0.23331200642797256
1103	46-47	-0.12159560889583076
1103	48-49	-0.29889267186378987
1103	50-51	-0.36839720527390796
1103	52-53	-0.1642161969743512
1103	54-55	-0.11523302680040359
1103	56-57	0.050101642015818015
1103	58-59	-0.02563213975463441
1103	60-61	0.01288400085895347
1103	62-63	0.033596793753318366
1103	64-65	-0.10390316910228847
1103	66-67	-0.005676356197646726
1103	68-69	-0.13401580504984878
1103	70-71	-0.07748576801626683
1103	72-73	-0.12076657775444488
1103	74-75	-0.10908032680214319
1103	76-77	-0.13013317523112988
1103	78-79	-0.18050128464995296
1103	80-81	-0.0888203460118362
1103	82-83	-0.10019941650787345
1103	84-85	-0.1508412810861195
1103	86-87	-0.8369533483502138
1103	88-89	-0.28740461080933954
1103	90-91	-0.5061142420292626
1103	92-93	-0.22993636843146703
1103	94-95	-0.6468476668939083
1103	96-97	-0.3798043600794081
1103	98-99	-0.3560688039823283
1103	100	-0.4512248478694829
1104	1	0.1031076441141181
1104	2	-0.0017107350455880521
1104	3	0.05590934832734362
1104	4	0.07234020508048644
1104	5	-0.002285595933628315
1104	6	0.11354851655711684
1104	7	-0.010372815667842872
1104	8	0.03599779800228475
1104	9	0.044642641742456135
1104	10-11	-0.07073435303541231
1104	12-13	0.002036858142069775
1104	14-15	-0.0853171570682818
1104	16-17	-0.011423594023305839
1104	18-19	0.04839969564746838
1104	20-21	-0.07669485881005755
1104	22-23	-0.0790601320170694
1104	24-25	-0.051793376219642084
1104	26-27	-0.049742706939177594
1104	28-29	-0.05604876222203359
1104	30-31	-0.03278041850995095
1104	32-33	-0.023644545749348822
1104	34-35	-0.13687163603180608
1104	36-37	-0.14714862968958897
1104	38-39	-0.07300828580874708
1104	40-41	-0.03756349189819019
1104	42-43	-0.23695413486559147
1104	44-45	-0.12523503636442967
1104	46-47	-0.03015422150759406
1104	48-49	-0.05975654107958661
1104	50-51	-0.2626753587972175
1104	52-53	-0.1757233434733081
1104	54-55	-0.04309014155882096
1104	56-57	-0.0374799622997628
1104	58-59	-0.11001612228067614
1104	60-61	-0.19192774884754016
1104	62-63	-0.016831268371987562
1104	64-65	-0.08525340788089153
1104	66-67	7.387998316730204E-4
1104	68-69	-0.10138138356837345
1104	70-71	-0.06006219500296339
1104	72-73	-0.04150981930263242
1104	74-75	-0.04600755116286592
1104	76-77	-0.10894281125192862
1104	78-79	-0.16354838833379048
1104	80-81	-0.17323319860637554
1104	82-83	-0.11799813053364971
1104	84-85	-0.1119667605046395
1104	86-87	-0.09882707309482441
1104	88-89	-0.1187247481339
1104	90-91	-0.22416881795532007
1104	92-93	-0.17723453129971745
1104	94-95	-0.2622586260747575
1104	96-97	-0.29366358705430784
1104	98-99	-0.2823323534015181
1104	100	-0.22221976038307645
1105	1	0.18041275055622563
1105	2	0.12787167421920032
1105	3	0.15938775039447606
1105	4	0.2102797988358347
1105	5	0.22913333906660682
1105	6	0.24037281319266413
1105	7	0.14284716276101506
1105	8	0.12097744683323697
1105	9	0.14011709391461835
1105	10-11	0.16123560012229632
1105	12-13	0.2271505863643526
1105	14-15	0.15919846735175014
1105	16-17	0.17358317918120036
1105	18-19	0.1494758270223926
1105	20-21	0.07951881557530527
1105	22-23	0.11268552238711749
1105	24-25	0.15373782721256646
1105	26-27	0.13062711676602845
1105	28-29	0.01799881205190701
1105	30-31	0.006679701895201617
1105	32-33	0.0626241058342103
1105	34-35	0.0024626447158055953
1105	36-37	0.02192914588822248
1105	38-39	0.05778660408014957
1105	40-41	0.01364339698135808
1105	42-43	-0.021886760627964463
1105	44-45	-0.0380725881355346
1105	46-47	-0.03965593529306233
1105	48-49	-0.008214428316854594
1105	50-51	-0.09309401867612621
1105	52-53	0.05148771683163744
1105	54-55	0.1500021579002535
1105	56-57	0.11283372628022903
1105	58-59	0.1602174489812498
1105	60-61	0.159162381945265
1105	62-63	0.026488800412622027
1105	64-65	0.07446452002769632
1105	66-67	0.12734839861343517
1105	68-69	0.11866797835233456
1105	70-71	0.15192466549373052
1105	72-73	0.08181063831013091
1105	74-75	0.056891713572191804
1105	76-77	0.1479150850562192
1105	78-79	0.07023307880092489
1105	80-81	0.13266989996353828
1105	82-83	0.10294861145549916
1105	84-85	0.08422982630089493
1105	86-87	0.02516377880036913
1105	88-89	0.03290546067911748
1105	90-91	-0.014987042346707113
1105	92-93	0.023398935005353394
1105	94-95	0.05661475265359783
1105	96-97	-0.013480949070856951
1105	98-99	0.029267599019181745
1105	100	0.049170975751508195
1106	1	-0.032596591306191414
1106	2	-0.09705000337614678
1106	3	-0.01902957037269104
1106	4	0.06058422019806642
1106	5	-0.03941610152551078
1106	6	0.06407796178570635
1106	7	-0.002010129084069945
1106	8	-1.1536923120764015
1106	9	0.04079453687929657
1106	10-11	-5.984818564641614E-4
1106	12-13	-0.03491085518543002
1106	14-15	-0.02876017039016432
1106	16-17	-0.06287980535770288
1106	18-19	0.059739374363580566
1106	20-21	-0.033684602718047074
1106	22-23	-0.02133540985109761
1106	24-25	0.009469576000938673
1106	26-27	-0.046810035362810254
1106	28-29	-0.10008548448669075
1106	30-31	-0.07515321991204615
1106	32-33	-0.0070063832234268375
1106	34-35	-0.003329723531720674
1106	36-37	-0.0022288940548165215
1106	38-39	-0.03223010280530758
1106	40-41	-0.04996050873821645
1106	42-43	-0.020408663757258694
1106	44-45	-0.1633639163130951
1106	46-47	-0.17341593505883424
1106	48-49	-0.1083405100178183
1106	50-51	-0.18302185242231417
1106	52-53	-0.06595485794903055
1106	54-55	-0.08778124829311906
1106	56-57	-0.06743227186153433
1106	58-59	-0.009242785579559154
1106	60-61	-0.054420561771671316
1106	62-63	3.657909095551304E-4
1106	64-65	0.010956040644892084
1106	66-67	-0.00191435566524234
1106	68-69	-0.0543417525439267
1106	70-71	-0.05446266162193325
1106	72-73	-0.07242824011024851
1106	74-75	-0.014749348889885994
1106	76-77	-0.022664115559194897
1106	78-79	-0.005365714315789916
1106	80-81	-0.06171319939118902
1106	82-83	-0.02938384374936831
1106	84-85	-0.0790136999001021
1106	86-87	-0.11472078175281553
1106	88-89	-0.07611933915838165
1106	90-91	-0.11889914403563395
1106	92-93	-0.08199406587998226
1106	94-95	-0.12340509127739452
1106	96-97	-0.17663634927506067
1106	98-99	-0.0885952878012759
1106	100	-0.08695509647266064
1107	1	-0.048241671951167575
1107	2	-0.0433373409309219
1107	3	-0.024284085573089698
1107	4	0.1209610852149936
1107	5	0.057358071673341726
1107	6	0.12347577423116718
1107	7	0.10913338339101131
1107	8	0.16695166961461894
1107	9	0.12739508387768694
1107	10-11	0.10976694819981958
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2316	90-91	0.16582611860168228
2316	92-93	0.3552278721949378
2316	94-95	0.3478476180149528
2316	96-97	0.31553035516245487
2316	98-99	0.3405667588199073
2316	100	-0.008685900592812601
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	27536.0
1	2563.0
2	1548.0
3	1219.0
4	1018.0
5	964.0
6	1149.0
7	2796.0
8	5435.0
9	7607.0
10	11892.0
11	19869.0
12	30994.0
13	40391.0
14	48119.0
15	55895.0
16	65700.0
17	77864.0
18	93931.0
19	110567.0
20	129808.0
21	148461.0
22	169092.0
23	184503.0
24	195780.0
25	208118.0
26	227279.0
27	255411.0
28	294526.0
29	348495.0
30	420068.0
31	519087.0
32	657400.0
33	876636.0
34	1261402.0
35	2013229.0
36	2196369.0
37	2154144.0
38	1974304.0
39	424062.0
40	493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.05729926207633	17.07411683159509	11.700720998515965	24.167862907812612
2	16.509483250834656	19.991845184365044	39.474060278771454	24.024611286028847
3	19.671028685419135	25.863927383463615	28.779972875187422	25.685071055929832
4	13.089892956413744	15.547676779624265	36.32008595028289	35.04234431367911
5	13.608972901506261	36.50850778889486	33.67870693699886	16.20381237260002
6	31.573263115736417	34.62312324471606	18.992463653664995	14.811149985882526
7	28.868522032092248	31.254173889147808	21.820862588870014	18.056441489889927
8	27.235349618985204	33.500738674673016	20.41813833491025	18.84577337143153
9	27.962484869329067	15.567591633339998	19.827484734016796	36.64243876331414
10-11	25.745758233519933	25.106784793888693	28.113511381375783	21.03394559121559
12-13	26.196095334617326	22.90511142242348	27.98589919370344	22.91289404925575
14-15	23.563654041400696	24.367315733376792	25.986323119149834	26.08270710607268
16-17	23.20211137097771	26.931185584314893	26.57385669777022	23.292846346937175
18-19	22.95180283158352	26.359894874351326	28.204440405107494	22.48386188895766
20-21	23.720497960693372	25.771691571093598	27.95707342291957	22.550737045293463
22-23	23.441941481393318	25.51570825244512	27.8120378084868	23.23031245767477
24-25	23.20358507283472	25.53192222886015	27.93839760824408	23.32609509006105
26-27	23.098916843191468	25.5229715582851	28.101032645476508	23.27707895304692
28-29	22.993769487488553	26.07535439715632	27.95633942227339	22.974536693081742
30-31	23.710520917439194	25.700510426806428	27.89427141583618	22.694697239918195
32-33	23.331809708445842	25.77778259383346	27.954494184448585	22.93591351327212
34-35	23.536699718633066	25.9193376706235	27.826119759203934	22.717842851539505
36-37	23.544466703663947	25.86025075802066	27.7742262403955	22.8210562979199
38-39	23.405868877575596	26.103510403906803	27.608159190643843	22.882461527873758
40-41	23.38382574382906	25.897854335546068	27.7370084319822	22.981311488642664
42-43	23.20938255771134	26.049180050654673	27.39286037359423	23.348577018039755
44-45	23.079164634739293	26.079875149977415	27.55237054108352	23.288589674199773
46-47	23.113055841152576	25.887693109587527	27.415586107469377	23.58366494179052
48-49	23.20969420155847	26.07355699430342	27.39699948603952	23.31974931809859
50-51	22.965567590365644	26.297191671166342	27.518683417210156	23.218557321257855
52-53	23.044670558163606	26.443437564854634	27.084559277463555	23.427332599518206
54-55	22.84102942294993	26.1536581018039	27.406600810592213	23.59871166465396
56-57	23.10201794646051	26.224112142209755	27.101360232061396	23.57250967926834
58-59	23.147566688321675	26.201024564384124	27.290402912855217	23.361005834438988
60-61	23.420434567598363	26.21460545165989	27.396987404057565	22.96797257668418
62-63	23.08489318621162	26.304916368318715	27.428415047075234	23.181775398394436
64-65	23.050069125715602	26.176362903324062	27.243915897653547	23.529652073306785
66-67	23.01110038293037	26.13864641010989	27.080740507202112	23.76951269975763
68-69	22.783502804833745	26.195779569449268	27.19267937769153	23.828038248025457
70-71	22.888252176105112	25.98877450440306	27.287851227067122	23.835122092424704
72-73	22.795921313370314	26.233972028743036	27.062315626053547	23.907791031833103
74-75	22.773948474382536	26.408095897362628	27.02568069357745	23.792274934677394
76-77	22.70913090747626	26.015366303656194	27.104906817852548	24.170595971015
78-79	22.668729003280387	26.064036878751146	27.186872619183923	24.080361498784544
80-81	22.78697946748742	26.17703901546503	26.85911576120017	24.17686575584738
82-83	22.9051082602031	26.08769348553605	26.953675925083875	24.053522329176978
84-85	22.862924233057488	26.326722376022747	26.69021263800948	24.12014075291028
86-87	22.657636581278616	26.26063973147797	26.93383004846264	24.147893638780776
88-89	22.510087208917522	25.976988297527065	27.06009598068916	24.452828512866258
90-91	23.064335105419467	25.899896265645346	26.90404665802646	24.131721970908725
92-93	22.903463734944147	25.95355913149454	26.916243171834402	24.226733961726907
94-95	22.76433899555207	25.738065553062935	26.963464531228716	24.53413092015628
96-97	22.65673109802527	25.66014665228813	27.127441384262646	24.555680865423955
98-99	22.73523410111226	25.664312414352615	26.860981808074186	24.739471676460944
100	22.714606081962994	25.548943107633292	26.894231140661716	24.842219669741993
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12887.0
1	14169.5
2	15895.5
3	12498.0
4	6600.0
5	5046.0
6	6685.5
7	10641.0
8	15946.0
9	18367.0
10	16362.5
11	14183.0
12	14484.0
13	15487.5
14	16497.0
15	17416.0
16	18192.0
17	19413.0
18	20880.0
19	22225.0
20	23865.0
21	26279.5
22	29571.0
23	34638.0
24	41074.5
25	48359.0
26	58121.0
27	70706.0
28	84580.5
29	100854.0
30	121562.5
31	141885.5
32	164297.5
33	190684.0
34	217917.5
35	246174.5
36	282006.5
37	316780.0
38	342520.5
39	368381.5
40	387745.0
41	404285.5
42	423944.0
43	448335.0
44	474918.0
45	502267.5
46	532754.5
47	567998.5
48	621457.5
49	655905.5
50	668252.5
51	664282.0
52	635401.5
53	615617.0
54	598620.5
55	565928.0
56	519431.5
57	472357.5
58	427146.0
59	374235.0
60	314921.0
61	260586.5
62	213541.5
63	169303.0
64	128969.0
65	97546.0
66	73392.0
67	57146.5
68	45157.0
69	34396.5
70	26994.5
71	21096.5
72	16654.0
73	13197.5
74	10160.5
75	7315.0
76	5169.0
77	3472.5
78	2214.5
79	1562.0
80	1134.5
81	798.0
82	583.5
83	418.5
84	307.5
85	234.0
86	181.0
87	154.5
88	123.5
89	86.5
90	70.5
91	63.5
92	49.0
93	32.5
94	28.0
95	23.5
96	20.0
97	19.5
98	12.5
99	10.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14504389048301936
2	0.07162450991515372
3	0.3139254974084426
4	0.31196686118522776
5	0.3138010355748604
6	0.2614419073736693
7	0.26278478505179315
8	0.26075409197755706
9	0.2730299591424553
10-11	0.24903830306377872
12-13	0.2587659779516517
14-15	0.23252745824567508
16-17	0.2407779676876118
18-19	0.2541805419775701
20-21	0.2553432775281408
22-23	0.2467062813398172
24-25	0.2461822315142079
26-27	0.2453732295959235
28-29	0.256309494394108
30-31	0.24171798206229853
32-33	0.25468166462330905
34-35	0.23793499738368126
36-37	0.24615275371151737
38-39	0.24707966684056384
40-41	0.2639933749621046
42-43	0.24962130849476905
44-45	0.23537042854960563
46-47	0.23856058186300239
48-49	0.24826532957100494
50-51	0.2413576978071921
52-53	0.2578587166910656
54-55	0.26730471479767354
56-57	0.26379685627750116
58-59	0.2609669872192108
60-61	0.2419210513697221
62-63	0.2689096173886021
64-65	0.26335796454855337
66-67	0.26873275057245893
68-69	0.2739929006970125
70-71	0.2937102753855631
72-73	0.3033920959136953
74-75	0.2829803552062123
76-77	0.27911876305375366
78-79	0.27653454235121766
80-81	0.26214282401542177
82-83	0.27306598756796596
84-85	0.284595083731371
86-87	0.27671795979018093
88-89	0.28560387964566897
90-91	0.27974762284448484
92-93	0.25904110411009657
94-95	0.28039613450367634
96-97	0.27887639000940934
98-99	0.2653526291972788
100	0.28775575924207725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.5265724E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.408800245621165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.13336319927419	35.54063259019464
2	16.932078212397712	17.409156531287767
3	6.283456339020887	9.690748553544205
4	2.860035063132427	5.881238850241898
5	1.4673505832779894	3.7717366513016928
6	0.8572252563505295	2.644135318153546
7	0.5637831516571024	2.02883907977707
8	0.35890335756780456	1.4760632813348809
9	0.2631177348251491	1.217391036363577
>10	1.1794631756014144	10.477211680011926
>50	0.06177062618100322	2.1872096173367725
>100	0.034860989924489307	3.447295787641713
>500	0.0029902351875513737	1.0299903095760383
>1k	0.0013647314026942955	1.2209117350303804
>5k	1.1867209949320573E-4	0.419297146419346
>10k+	1.1867209949320573E-4	1.5581418317845765
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46424	0.304106113801088	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34680	0.2271755994016399	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23884	0.15645507543566228	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	19639	0.12864768156426776	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15778	0.103355726855798	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3101245640232983E-5
2	0.0	0.0	0.0	0.0	1.3101245640232983E-5
3	0.0	0.0	0.0	0.0	1.3101245640232983E-5
4	0.0	0.0	0.0	0.0	1.9651868460349474E-5
5	0.0	0.0	0.0	0.0	2.6202491280465966E-5
6	0.0	0.0	0.0	0.0	3.2753114100582454E-5
7	0.0	0.0	0.0	0.0	3.930373692069895E-5
8	0.0	0.0	0.0	0.0	3.930373692069895E-5
9	0.0	0.0	0.0	0.0	5.240498256093193E-5
10-11	0.0	0.0	0.0	3.2753114100582457E-6	5.240498256093193E-5
12-13	0.0	0.0	0.0	1.637655705029123E-5	6.550622820116491E-5
14-15	0.0	0.0	0.0	2.2927179870407718E-5	1.801421275532035E-4
16-17	3.2753114100582457E-6	0.0	0.0	2.6202491280465966E-5	2.0306930742361123E-4
18-19	6.550622820116491E-6	0.0	0.0	5.8955605381048426E-5	2.227211758839607E-4
20-21	9.825934230174737E-6	0.0	0.0	1.441137020425628E-4	2.3582242152419365E-4
22-23	1.3101245640232983E-5	0.0	0.0	4.094139262572807E-4	3.275311410058246E-4
24-25	1.3101245640232983E-5	0.0	0.0	8.876093921257845E-4	4.2906579471763015E-4
26-27	1.3101245640232983E-5	0.0	0.0	0.00199793996013553	4.454423517679214E-4
28-29	1.3101245640232983E-5	0.0	0.0	0.005263425435963601	4.487176631779796E-4
30-31	1.3101245640232983E-5	0.0	0.0	0.016789246287958567	4.6509422022827083E-4
32-33	1.3101245640232983E-5	0.0	0.0	0.04569059417031253	5.20774514199261E-4
34-35	1.3101245640232983E-5	0.0	0.0	0.09193144065751484	5.568029397099017E-4
36-37	1.3101245640232983E-5	0.0	0.0	0.1600218895612157	5.633535625300182E-4
38-39	1.3101245640232983E-5	0.0	0.0	0.25952912551019525	5.731794967601929E-4
40-41	1.3101245640232983E-5	0.0	0.0	0.3766182331083675	5.99381988040659E-4
42-43	1.3101245640232983E-5	0.0	0.0	0.48750390089588935	6.288597907311832E-4
44-45	1.3101245640232983E-5	0.0	0.0	0.6210154199040936	6.779894618820568E-4
46-47	1.3101245640232983E-5	0.0	0.0	0.7758393902575469	7.303944444429888E-4
48-49	1.3101245640232983E-5	0.0	0.0	0.9383930955387376	7.59872247133513E-4
50-51	1.637655705029123E-5	0.0	0.0	1.1053881230919673	7.696981813636876E-4
52-53	2.6202491280465966E-5	0.0	0.0	1.278111670301389	8.679575236654351E-4
54-55	2.6202491280465966E-5	3.2753114100582457E-6	0.0	1.4787310447902766	9.694921773772407E-4
56-57	2.6202491280465966E-5	6.550622820116491E-6	0.0	1.7094472558261895	9.793181116074154E-4
58-59	3.930373692069895E-5	6.550622820116491E-6	0.0	1.9591864755317205	9.956946686577066E-4
60-61	3.930373692069895E-5	1.9651868460349474E-5	0.0	2.2155025205486485	0.0010055206028878813
62-63	4.257904833075719E-5	1.9651868460349474E-5	0.0	2.4844874700996824	0.0010874033881393375
64-65	4.5854359740815436E-5	1.9651868460349474E-5	0.0	2.757871162874424	0.0010972293223695123
66-67	4.5854359740815436E-5	1.9651868460349474E-5	0.0	3.0607785126994305	0.0011234318136499784
68-69	4.5854359740815436E-5	1.9651868460349474E-5	0.0	3.405652427621513	0.0011430836821103277
70-71	4.5854359740815436E-5	1.9651868460349474E-5	0.0	3.776332521143445	0.0011791121076209685
72-73	4.5854359740815436E-5	1.9651868460349474E-5	0.0	4.127422977121819	0.0012904726955629488
74-75	4.5854359740815436E-5	2.2927179870407718E-5	0.0	4.480491721191868	0.0012904726955629488
76-77	4.5854359740815436E-5	2.6202491280465966E-5	0.0	4.86806259565547	0.0012970233183830652
78-79	4.5854359740815436E-5	2.6202491280465966E-5	0.0	5.304468363243041	0.0013101245640232983
80-81	4.5854359740815436E-5	2.6202491280465966E-5	0.0	5.750742644109117	0.0013494283009439973
82-83	5.240498256093193E-5	2.6202491280465966E-5	0.0	6.208483790221807	0.001441137020425628
84-85	6.550622820116491E-5	2.6202491280465966E-5	0.0	6.669798301082871	0.001441137020425628
86-87	6.550622820116491E-5	2.6202491280465966E-5	0.0	7.153758315033077	0.0014444123318356863
88	7.20568510212814E-5	2.6202491280465966E-5	0.0	7.533393109950108	0.0014607888888859776
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	27570	0.0	54.783085	2
GTACCTG	32185	0.0	47.552475	1
ACCTGGG	31645	0.0	47.41649	3
CCTGGGG	28440	0.0	35.327946	4
GTACCCG	3050	0.0	27.757591	1
TATCACG	1330	0.0	26.163015	2
TATAACG	1595	0.0	25.35394	2
CTGGGGG	18335	0.0	25.205128	5
GTACATG	65965	0.0	24.819872	1
CATGGGG	38570	0.0	24.793896	4
TGGGGGG	24965	0.0	23.736448	6
TACATGG	68065	0.0	23.703068	2
ATGGGGG	23860	0.0	23.467003	5
ACATGGG	68475	0.0	22.929369	3
ATAACGC	1780	0.0	22.71884	3
TAACGCA	1850	0.0	21.347696	4
TACCCGG	3670	0.0	21.269129	2
GTATCAA	78205	0.0	20.682676	1
GTACACG	3235	0.0	20.500002	1
GAGTACT	41530	0.0	18.517	12-13
>>END_MODULE