FastQCFastQC Report
Mon 6 Jun 2016
ERR522874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15265724
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427740.2801963405076628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339760.22256396093627792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237540.15560349446904712No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT205920.13489042511183877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG284450.056.290562
GTACCTG327350.049.325581
ACCTGGG325700.048.435433
CCTGGGG287550.037.3424154
TATAACG14950.029.8642642
CTGGGGG179100.027.3400215
TAACGCA17300.026.3484574
TGGGGGG235100.025.484986
ATAACGC18350.025.3529623
GTACCCG27650.024.6579571
CATGGGG375700.024.2155174
GTACATG670550.023.119161
ATGGGGG231150.023.1149675
TACATGG685850.022.283882
ACATGGG679000.021.7314873
TACCCGG30800.020.7518862
GTATCAA804450.019.6859911
GTACACG31750.018.2156931
GAGTACT431100.018.05599412-13
AGTACTT457150.017.53092412-13
Produced by FastQC (version 0.11.5)