FastQCFastQC Report
Mon 6 Jun 2016
ERR522873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10196130
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT520320.5103112651564858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363690.356694157489165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268160.2630017467411655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162930.1597959225706224No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT150860.1479580978273129No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC119310.11701498509728692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG389550.027.4356441
TACATGG400500.026.224822
ACATGGG408150.025.0083333
CATGGGG273600.022.8018254
GAGTACT300650.021.6666212-13
ATGGGGG163700.020.8794825
AGAGTAC477800.020.36285410-11
GTACTTT325650.019.77839714-15
AGTACTT318900.019.24071312-13
GAGTACA307450.018.5203231
AGTACAT295800.018.0079212
CATGGGA134450.016.7490224
GTATCAA925600.016.540171
ACTTTTT403100.016.19286716-17
GTGTAGC51100.015.6425291
TACTTTT389800.015.62555314-15
TCAACGC973650.015.5670724
CAACGCA976250.015.5166695
AACGCAG988900.015.3402016
ATCAACG990350.015.3151193
Produced by FastQC (version 0.11.5)