FastQCFastQC Report
Mon 6 Jun 2016
ERR522873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10196130
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT528540.5183731474588887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334740.32830103186208887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263660.25858830752452155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155780.1527834580375103No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT150780.14787963668568369No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC124100.12171284595233682No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC113140.11096366954913287No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG392300.027.4667781
TACATGG401750.026.1841682
ACATGGG405500.025.2301063
CATGGGG279150.023.5017014
ATGGGGG159250.021.8077135
GAGTACT302250.021.17017212-13
AGAGTAC483550.020.31568110-11
GTACTTT329500.019.26591914-15
AGTACTT323550.019.09354212-13
GAGTACA304750.017.3315321
AGTACAT293550.016.7007832
ACTTTTT399650.015.95763816-17
GTATCAA917350.015.7297271
TGGGGGG154850.015.6900536
TACTTTT391750.015.51458214-15
CATGGGA134500.015.5135854
GTATAGG32150.015.506931
TATACCG7150.015.1170455
GTATATA55250.014.72701551
TCAACGC974000.014.6919634
Produced by FastQC (version 0.11.5)