FastQCFastQC Report
Mon 6 Jun 2016
ERR522872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13351
Sequences flagged as poor quality0
Sequence length100
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCAGC200.002116319370.499996
CAGCTGA250.005123723356.3999989
CGCTGCC1151.2803324E-424.521748
ACGCTGC1351.3224089E-524.370377
GACGCTG1351.3224089E-524.370376
TGACGCT1401.7528517E-523.55
GCTGCCG1252.2540102E-422.5599989
CTGACGC1559.624135E-418.1935484
GACGAAG650.00784046418.07692314-15
TCTGACG1600.001191124217.6249983
CCGACGA1002.8575442E-416.44999912-13
TGCCGAC1154.407412E-516.34782610-11
GCCGACG1200.001140845513.70833312-13
Produced by FastQC (version 0.11.5)