FastQCFastQC Report
Mon 6 Jun 2016
ERR522872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13351
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG11728.77836866152348TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG8696.508875739644971RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG8246.1718223354055874RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG6805.093251441839563TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA3272.4492547374728484RNA PCR Primer, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA2692.014830349786533RNA PCR Primer, Index 46 (96% over 28bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA2501.8725189124410155RNA PCR Primer, Index 26 (100% over 29bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA2351.7601677776945546RNA PCR Primer, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG1691.2658227848101267RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1220.9137892292712156TruSeq Adapter, Index 3 (96% over 28bp)
TCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAA530.39697400943749533TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG500.37450378248820315RNA PCR Primer, Index 23 (96% over 30bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA460.3445434798891469RNA PCR Primer, Index 33 (96% over 29bp)
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA400.2996030259905625TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA380.28462287469103437RNA PCR Primer, Index 3 (96% over 28bp)
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT370.2771327990412703TruSeq Adapter, Index 4 (96% over 26bp)
GAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAA360.26964272339150624Illumina PCR Primer Index 5 (96% over 27bp)
TCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAA330.24717249644221403TruSeq Adapter, Index 4 (96% over 27bp)
TCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA300.22470226949292188RNA PCR Primer, Index 26 (96% over 31bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG300.22470226949292188RNA PCR Primer, Index 31 (96% over 29bp)
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT290.21721219384315782TruSeq Adapter, Index 1 (96% over 27bp)
GAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAA280.20972211819339373TruSeq Adapter, Index 18 (96% over 27bp)
GAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAA270.2022320425436297Illumina PCR Primer Index 9 (96% over 27bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG270.2022320425436297TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG260.19474196689386564RNA PCR Primer, Index 39 (96% over 30bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT210.15729158864504533RNA PCR Primer, Index 26 (96% over 30bp)
GAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAA190.14231143734551718RNA PCR Primer, Index 26 (96% over 31bp)
TCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGTCTTCTGCTTG180.13482136169575312Illumina PCR Primer Index 5 (96% over 26bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGCCTTCTGCTTG170.12733128604598906Illumina PCR Primer Index 8 (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT170.12733128604598906No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT160.11984121039622501TruSeq Adapter, Index 1 (96% over 26bp)
TCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGTCTTCTGCTTG140.10486105909669687TruSeq Adapter, Index 5 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA6100.073.1967241
CTCCGAG7500.059.5333372
GGTATCA250.005123723356.3999981
TCCGAGC8050.055.4658363
CCGAGCC8200.054.451224
CGAGCCC8250.054.1212125
CCCACGA8200.053.878059
GCCCACG8250.053.5515178
AGCCCAC8450.052.2840237
GAGCCCA8550.052.2222256
ACTAAGG1800.040.4722216-17
AGACTAA1800.040.4722214-15
TAAGGCG1800.040.4722218-19
CGAGACT5350.040.41121312-13
GCGAATC1700.040.08823822-23
CATGATC2350.040.022-23
ACTAGGC2550.039.62745316-17
AGGCGAA1850.039.3783820-21
AGACTAG2600.038.86538714-15
TAGGCAT2600.038.86538718-19
Produced by FastQC (version 0.11.5)