FastQCFastQC Report
Mon 6 Jun 2016
ERR522871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12200461
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT681320.5584379147640405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315990.2589984099781148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263500.21597544551800132No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT178080.1459616976768337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149610.12262651386697601No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC141300.11581529583185424No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC131540.10781559811551383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG141550.036.27692
GTACCTG161900.032.307281
ACCTGGG171400.029.630643
GTACATG553750.021.4736461
TACATGG563900.020.6891232
CATGGGG322350.020.2174384
GAGTACT291000.019.64316712-13
CCTGGGG187800.019.5294324
ACATGGG579400.019.5030883
GTACTTT318600.017.92785614-15
ATGGGGG171800.017.3238495
TATAACG9000.017.2420462
GTATAGG31250.017.159211
AGTACTT310650.016.97125212-13
GTACACG26650.015.1790191
TACACGG25000.015.0476042
AGAGTAC627450.014.96882410-11
ACTTTTT398200.014.810286516-17
GTATATA46700.014.4032871
TACTTTT369300.014.21960214-15
Produced by FastQC (version 0.11.5)