FastQCFastQC Report
Mon 6 Jun 2016
ERR522871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12200461
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT711610.5832648454841174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291220.23869589845826317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261190.2140820744396462No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT178770.14652725007686185No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC155230.12723289718314743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148030.12133148083502746No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC144040.11806111260877765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG147200.036.142772
GTACCTG169950.031.9825841
ACCTGGG179350.029.267563
GTACATG556500.019.6526621
CATGGGG320650.019.4040094
TACATGG570300.019.1603032
GAGTACT302150.019.1032612-13
CCTGGGG183100.018.8639984
ACATGGG576300.018.175913
TATAACG10100.018.1478862
ATGGGGG164750.017.8844285
GTACTTT326750.017.61291914-15
AGTACTT321250.016.93595112-13
GTATAGG30250.015.3881591
ACTTTTT390250.014.968554516-17
TACTTTT369950.014.55258714-15
AGAGTAC657000.014.48373710-11
GTATACG7200.014.3670311
GTCTTAG47200.014.0460421
TAACGCA13500.013.92390254
Produced by FastQC (version 0.11.5)