FastQCFastQC Report
Mon 6 Jun 2016
ERR522870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593968
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT557360.996358935195911No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT160840.2875239901265077No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC122640.21923614865154753No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC113500.20289712061277435No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG96930.17327592864313845No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA95280.17032632292497918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG207750.025.2070751
TACATGG213500.024.3064862
ACATGGG217000.022.8332083
CATGGGG146550.020.8490524
TACCTGG41900.018.734992
ATGGGGG83500.018.2405055
GAGTACA164400.017.5281851
AGTACAT152750.017.2328782
GTGTAGC35450.016.3104951
GTATATA17300.015.4884171
GTACCTG54250.015.3374021
TAGCCCT42600.014.8964564
TATACCG3504.9967093E-714.7741645
GTATAAT20450.014.4818981
TGGGGGG66100.014.4283146
AGAGTAC154150.014.30816310-11
CATGGGA72950.014.2405184
GTATAGA18400.014.0515151
GTATTAA24000.013.9067421
GTATTAC10900.013.8007211
Produced by FastQC (version 0.11.5)