FastQCFastQC Report
Mon 6 Jun 2016
ERR522870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593968
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT566181.0121259184893443No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT154710.2765657579735887No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC127350.22765593224702038No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC119280.21322967882547772No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG97460.17422337775260782No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA95470.17066597449252482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG203400.025.0650221
TACATGG208050.023.8794962
ACATGGG211500.023.0866533
CATGGGG145100.021.149764
ATGGGGG77200.018.992985
GTATATA16700.018.587341
TACCTGG43100.018.5391372
TGGGGGG59900.016.4758666
GAGTACA163750.016.1989751
GTGTAGC35450.015.9204291
AGTACAT151450.015.6415262
GTACCTG53550.015.3698041
CATGGGA73600.014.7500614
AGAGTAC152450.014.54081210-11
GTATAAG16550.014.4931221
TAGCCCT39950.014.3516794
TACACCG7250.014.2606875
GTATAGC19800.014.0144671
GTATAAT19400.013.8185621
TAGACAG28000.013.4272715
Produced by FastQC (version 0.11.5)