FastQCFastQC Report
Mon 6 Jun 2016
ERR522869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8183651
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA239720.2929254925460531No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT156490.19122271954168135No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG116380.14221036552023053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG71100.025.861912
CATGGGG174450.023.0198764
ACCTGGG91450.020.0042363
ATGGGGG90300.019.4748175
GTATAGG42800.019.4538461
GTACATG715250.018.993951
GTCGCCG14150.018.93645994
ACATGGG703850.018.4142993
TACATGG730000.018.3601152
GTACCTG105600.018.3085461
ATGGGAT107250.016.83545
ATGGGCG12850.016.4664085
TATACCG4601.2732926E-1116.3550425
GGTCGCC18400.016.3509394
CTATTAA61500.016.2157361
TCGCCGT7600.016.08294
ACCCTTG49850.015.9313577
GTGTAGC49600.015.5538571
CTGGTCG25700.015.5416019
GTACACG12300.015.2978651
Produced by FastQC (version 0.11.5)