FastQCFastQC Report
Mon 6 Jun 2016
ERR522869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8183651
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT159630.19505963780713523No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG119930.14654828266747935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG70450.023.8832862
CATGGGG168350.022.1919574
GTATACG3850.020.7607081
GTATAGG41000.019.7241611
ATGGGGG85850.018.9943945
ACCCTTG48500.018.3127567
GTACATG711850.018.2691211
ACATGGG690550.017.8502713
ACCTGGG91150.017.8386123
TACATGG728400.017.5828232
GTACCGG4355.456968E-1217.2935711
GTGTAGC49450.016.82911
GTACCTG104950.016.754951
CTATTAA59200.016.5988711
TAGCCCT76550.015.2860684
AACCCTT58450.015.1953576
CATGGGT63400.015.1210714
ATGGGAT108700.014.8718585
GTATAGT38650.014.5977421
CTACACG26750.014.5812894
Produced by FastQC (version 0.11.5)