FastQCFastQC Report
Mon 6 Jun 2016
ERR522868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10711189
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416200.38856563916480236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327390.3056523416774739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212260.19816660876771008No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189010.17646033507577918No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113730.10617868847240021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG192200.055.6842962
GTACCTG226650.047.961911
ACCTGGG221550.047.628513
CCTGGGG202900.036.2659764
TATAACG10500.032.2446022
CTGGGGG131650.026.9626545
ATAACGC13100.024.7681583
CATGGGG303150.024.381594
TAACGCA14150.024.2567984
GTACATG499900.024.107281
ATGGGGG196550.024.0875685
TGGGGGG212200.023.9382926
GTATCAA577950.023.4072741
TACATGG510450.023.260662
GTACACG25100.022.6758671
ACATGGG519100.022.3568173
GGTATCA432050.021.1321531
GTACCCG19050.020.7413251
TCAACGC656450.020.384514
CAACGCA659750.020.2745
Produced by FastQC (version 0.11.5)