FastQCFastQC Report
Mon 6 Jun 2016
ERR522868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10711189
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373010.3482433182721358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319860.29862230981079696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210880.19687823639373742No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172290.1608504900809798No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121350.11329274462433629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG202400.056.1221922
GTACCTG233050.049.2516061
ACCTGGG229800.048.730853
CCTGGGG203250.038.172144
CTGGGGG128200.027.931675
TATAACG11250.025.900472
TGGGGGG190250.024.8486796
TATCACG12050.023.7909222
CATGGGG295650.022.8247764
ATGGGGG186500.022.677425
GTACATG501900.022.5788351
GTATCAA588750.021.907691
ATAACGC14250.021.7650183
TACATGG514850.021.7435052
TAACGCA13800.021.4531674
GGTATCA448550.021.2807461
ACATGGG518550.020.943023
GTATAAC26250.019.7044851
GAGTACT350900.019.1897812-13
TCAACGC667300.019.07034
Produced by FastQC (version 0.11.5)