FastQCFastQC Report
Mon 6 Jun 2016
ERR522867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6524394
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226860.34771045402837414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202430.31026636343543934No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT157920.2420454681308333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108860.16685074506536546No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG60650.028.6904812
TATAACG6150.024.470442
GTACCTG75750.024.1503751
GTACATG217450.023.9195291
TACATGG220000.022.8946322
ACCTGGG74750.022.7120463
ACATGGG220650.021.5906523
GTATCAA388900.020.3759961
GAGTACT196750.020.30592712-13
CATGGGG141700.020.1122044
GGTATCA308150.019.885621
AGAGTAC303450.018.74132210-11
GTACTTT214800.018.49053214-15
ATAACGC7650.018.4425133
TCAACGC427700.018.3186174
AGTACTT209200.018.16506612-13
CAACGCA430600.018.1627585
ATCAACG434950.017.9485343
AACGCAG441350.017.7982336
TATCAAC443600.017.683632
Produced by FastQC (version 0.11.5)