FastQCFastQC Report
Mon 6 Jun 2016
ERR522867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6524394
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221800.3399549444745366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207260.3176693498277388No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT159970.2451875223967161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109360.16761709976436126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG62450.025.8176882
TATAACG6100.023.1178322
GTACCTG70900.022.8220311
GTACATG216700.022.4007641
TACATGG218750.021.7034632
GAGTACT200450.021.17178512-13
ACATGGG221150.020.7439733
TAACGCA7100.020.5226024
GGTATCA312450.020.2029151
GTATCAA401100.020.1822811
ATAACGC7250.020.0979963
ACCTGGG76150.020.0605373
CATGGGG139050.019.741084
GTACTTT215200.019.40346714-15
AGAGTAC306900.018.62963510-11
TCAACGC432550.018.4731794
CAACGCA436850.018.3134275
ATCAACG439900.018.1645223
AGTACTT211300.018.1050912-13
AACGCAG444250.018.0590616
Produced by FastQC (version 0.11.5)