##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522866_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 750723 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.564276037899464 29.0 27.0 30.0 25.0 30.0 2 32.06285407533804 34.0 34.0 34.0 26.0 35.0 3 31.10472038288423 34.0 33.0 34.0 23.0 34.0 4 31.57700510041653 34.0 32.0 35.0 25.0 35.0 5 31.88380001678382 35.0 33.0 35.0 23.0 36.0 6 32.2629305349643 35.0 34.0 36.0 25.0 36.0 7 32.41344810269567 36.0 33.0 36.0 24.0 36.0 8 32.875400114289825 35.0 34.0 36.0 23.0 38.0 9 32.95540165946694 36.0 33.0 37.0 23.0 38.0 10-11 33.075906825819914 36.0 34.0 37.0 22.5 37.0 12-13 33.22269931785759 36.0 34.5 36.5 23.5 38.5 14-15 33.212246727488036 36.0 34.0 37.0 22.5 38.0 16-17 33.29317404688547 36.5 34.0 37.0 24.0 38.0 18-19 33.39181429102345 36.0 34.5 37.5 24.0 37.5 20-21 33.73304534428811 37.0 34.5 38.0 23.5 38.0 22-23 33.7451283629248 37.0 34.0 38.0 23.5 38.0 24-25 33.58706673433477 36.5 35.0 37.5 23.5 38.0 26-27 33.675322322614335 37.0 35.0 38.0 23.0 38.0 28-29 33.62850079190327 37.0 34.0 37.5 22.5 38.0 30-31 33.66036940389465 37.5 33.5 38.0 23.5 38.5 32-33 33.511311762127974 37.5 34.0 37.5 20.5 38.5 34-35 33.104861580103446 36.5 33.5 37.5 20.5 39.0 36-37 32.66710957303826 36.5 33.0 37.0 15.5 37.5 38-39 32.55116667532499 36.5 33.0 37.5 12.5 38.0 40-41 32.27952120822194 37.0 32.5 37.5 11.0 39.0 42-43 31.391144936281425 36.5 31.5 37.5 10.5 38.0 44-45 30.822304631668402 36.0 29.5 37.5 10.5 38.0 46-47 29.760487556662042 36.0 22.0 37.0 10.0 38.0 48-49 29.037904127088154 35.5 22.0 37.5 8.0 38.0 50-51 28.49529720016571 35.5 20.5 37.0 6.5 38.5 52-53 28.091749553430496 36.0 20.0 37.0 7.0 38.5 54-55 28.034707874941887 34.5 12.0 38.0 7.0 38.5 56-57 27.761525223018346 35.5 11.5 37.5 7.0 38.0 58-59 27.602338678846927 35.5 12.0 37.0 7.0 38.5 60-61 27.189376774123076 35.5 12.0 37.0 7.0 38.0 62-63 26.94939678150263 35.0 11.0 36.5 7.0 37.5 64-65 26.753077366751782 34.0 11.0 36.5 7.0 38.0 66-67 26.8456507926359 34.0 11.0 37.0 7.0 38.0 68-69 26.535979315939436 34.5 11.0 37.5 7.0 38.0 70-71 25.948637513437045 33.5 10.5 36.5 7.0 38.0 72-73 25.834995064757575 33.5 11.0 37.0 7.0 38.0 74-75 25.401259186144557 33.0 11.0 37.0 6.5 38.0 76-77 25.00975526259353 32.0 11.0 37.0 6.5 37.5 78-79 24.85718966915893 31.5 10.5 36.5 7.0 37.5 80-81 24.64650876555001 31.0 10.5 37.0 6.5 37.0 82-83 24.603223159540867 30.5 10.0 37.0 6.0 38.0 84-85 24.16204045966355 29.5 10.5 37.0 6.0 37.0 86-87 24.018569432400497 28.5 10.5 37.0 6.5 38.0 88-89 23.771639472881475 26.5 10.0 37.0 6.5 37.5 90-91 23.495855328796374 26.0 10.5 36.0 6.0 38.0 92-93 23.360559753730737 23.0 9.5 37.0 6.5 37.0 94-95 23.144944273720135 24.5 10.0 36.0 6.0 37.5 96-97 23.119153136376532 22.5 10.5 36.0 6.0 37.0 98-99 23.189389428590836 24.0 10.5 36.0 7.0 37.5 100 22.848107757455146 21.0 11.0 35.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20686361909658402 1101 2 -0.3171080796899979 1101 3 0.3199867173927622 1101 4 0.04869143662461184 1101 5 -0.03361282870393367 1101 6 0.09040725270462246 1101 7 -0.006144708457036074 1101 8 0.09015411018005892 1101 9 0.4252691580890229 1101 10-11 -0.11883634374696328 1101 12-13 -0.1376977972951252 1101 14-15 -0.18003262957907396 1101 16-17 0.2883768830349709 1101 18-19 -0.05698020466379461 1101 20-21 0.14429946449235587 1101 22-23 -0.032543313850609934 1101 24-25 0.014914172923660374 1101 26-27 0.1393620615050608 1101 28-29 -0.06433930350416261 1101 30-31 -0.010540255650646202 1101 32-33 0.11658342534559551 1101 34-35 -0.5566207099289286 1101 36-37 0.280391926765418 1101 38-39 -0.16498040093603095 1101 40-41 -0.1987126842911877 1101 42-43 0.21296397400112 1101 44-45 -0.15851148826515882 1101 46-47 -0.24670096726556068 1101 48-49 -0.11983866451470604 1101 50-51 -0.42211586679806956 1101 52-53 -0.6088294478756957 1101 54-55 -0.4734760448838031 1101 56-57 -0.3925296789671897 1101 58-59 -1.3045645659994065 1101 60-61 -0.5573271037185101 1101 62-63 -0.5698305686304685 1101 64-65 -0.2007654929899516 1101 66-67 -0.3960875182594883 1101 68-69 -0.5021267068117758 1101 70-71 -0.07337658612522446 1101 72-73 -0.2599487374429934 1101 74-75 -0.2823482575111598 1101 76-77 -0.563077603778833 1101 78-79 -0.24183727614325434 1101 80-81 -0.29876687385419487 1101 82-83 -1.075883257023662 1101 84-85 -1.2868923594241402 1101 86-87 -0.9361929847831902 1101 88-89 -1.40180818164038 1101 90-91 -0.7226563346827852 1101 92-93 -1.0462464141150996 1101 94-95 -1.142110822663824 1101 96-97 -0.7803921935160112 1101 98-99 -1.0138789067217715 1101 100 -1.2693540952728632 1102 1 -0.23378483377565829 1102 2 -0.16813959493297403 1102 3 0.19334531864145532 1102 4 0.007399870807109465 1102 5 0.09253520722835873 1102 6 -0.41740239050699657 1102 7 0.012463776222595868 1102 8 0.04797836080062723 1102 9 -1.6476421275370932 1102 10-11 0.05563706399708934 1102 12-13 -0.2941770054381294 1102 14-15 -0.26894628334672177 1102 16-17 -0.4643264549998207 1102 18-19 -0.17787054075028408 1102 20-21 0.08888321137981592 1102 22-23 -0.028388425012018104 1102 24-25 -0.254192027776341 1102 26-27 -0.10516789916134428 1102 28-29 -0.24445639494654614 1102 30-31 -0.1862257881245597 1102 32-33 0.04431157662804708 1102 34-35 -0.002380788557424296 1102 36-37 -0.14993556940859776 1102 38-39 -0.0963681434818966 1102 40-41 -0.4662730148249352 1102 42-43 -0.3873686105815821 1102 44-45 -0.7767366977508843 1102 46-47 -0.8611727498144468 1102 48-49 -0.6882864676958356 1102 50-51 -0.8204154096151903 1102 52-53 -0.6887561593126215 1102 54-55 -0.4866449626392537 1102 56-57 -0.30079128121615284 1102 58-59 -0.25590738350740594 1102 60-61 -0.2586201012555662 1102 62-63 -0.45740866022569193 1102 64-65 -0.4983280871883906 1102 66-67 -0.5536792111904667 1102 68-69 -0.4763518853414048 1102 70-71 -0.5968645258227454 1102 72-73 -0.7398282408297838 1102 74-75 -0.4601455763260809 1102 76-77 -0.46995005110993304 1102 78-79 -0.9752394867956369 1102 80-81 -1.014374164789281 1102 82-83 -1.119815764990019 1102 84-85 -0.8290323158147359 1102 86-87 -1.2266859799985355 1102 88-89 -1.2969502024157897 1102 90-91 -1.4285995186335967 1102 92-93 -1.3351625830657454 1102 94-95 -1.0653580786959118 1102 96-97 -1.1593896415726483 1102 98-99 -1.1698270243007727 1102 100 -1.1180000561791807 1103 1 0.09204191633548575 1103 2 -0.1384533476926073 1103 3 0.016096098558541883 1103 4 -0.10624763326821807 1103 5 -0.09881331786388259 1103 6 0.17811417869378232 1103 7 -0.002657685651712427 1103 8 -0.27803838129285197 1103 9 -0.2898264273596425 1103 10-11 -0.24079032676702639 1103 12-13 -0.4138026072377272 1103 14-15 -0.18423170830179458 1103 16-17 -0.14946034234976935 1103 18-19 -0.15505345363136058 1103 20-21 -0.3704290556425107 1103 22-23 -0.12867379296949366 1103 24-25 -0.2525248481313156 1103 26-27 -0.0387134535389535 1103 28-29 -0.6989286162188009 1103 30-31 -0.06316988108383725 1103 32-33 -0.07929055736190094 1103 34-35 0.046393953148474054 1103 36-37 0.18366660257974843 1103 38-39 0.2241635300814835 1103 40-41 0.4377021874215288 1103 42-43 -0.08238588085277598 1103 44-45 -0.10646303329952289 1103 46-47 -0.19426669325003232 1103 48-49 -0.46282203229500496 1103 50-51 -0.35156079527991224 1103 52-53 -0.2168949218568379 1103 54-55 -0.8283445929148527 1103 56-57 -0.38647671529083283 1103 58-59 -0.20566400377802907 1103 60-61 0.03760684796717939 1103 62-63 -0.016165580683647818 1103 64-65 0.1160280869810606 1103 66-67 0.07465372315970242 1103 68-69 -0.0942134115849953 1103 70-71 -0.18089702965108145 1103 72-73 -0.1983710403236465 1103 74-75 -0.04027659479710266 1103 76-77 -0.9055589184157355 1103 78-79 -0.8735168294917734 1103 80-81 0.06478504469207991 1103 82-83 -0.2481372998212521 1103 84-85 -0.20315692775611183 1103 86-87 -0.5917679235405231 1103 88-89 -0.35231101618945004 1103 90-91 -0.41404993262612777 1103 92-93 -0.29947068926366427 1103 94-95 -0.4337392330111989 1103 96-97 -1.1909710588368405 1103 98-99 -0.8600298977395155 1103 100 0.04237597595226461 1104 1 0.11326029878859956 1104 2 0.12317570336471562 1104 3 0.18942685859063246 1104 4 -0.2531074488987528 1104 5 0.28798034878764867 1104 6 0.20888783414388712 1104 7 0.25519466740522034 1104 8 -0.02420113502792276 1104 9 0.02994787898430218 1104 10-11 0.04754996961673896 1104 12-13 -0.09655619093813783 1104 14-15 -0.11164569192348495 1104 16-17 -0.03878000691238981 1104 18-19 0.13957250946447175 1104 20-21 0.12027867788457058 1104 22-23 -0.21784624956395504 1104 24-25 0.012960370523600773 1104 26-27 -0.13482057855598129 1104 28-29 -0.09449799696142946 1104 30-31 0.3063505009757108 1104 32-33 -0.6184085205182797 1104 34-35 0.008129342095394065 1104 36-37 0.11517327045737602 1104 38-39 0.44836807725614847 1104 40-41 0.4450247431637777 1104 42-43 0.3074806102239229 1104 44-45 0.5211140522097644 1104 46-47 0.03968234024829442 1104 48-49 0.29398207893215655 1104 50-51 0.16874557893863695 1104 52-53 0.0316670568604458 1104 54-55 -0.04146123914648214 1104 56-57 -0.07175251263579341 1104 58-59 0.045355856052736954 1104 60-61 0.4049966879683886 1104 62-63 0.16442340255961696 1104 64-65 0.5342254894605105 1104 66-67 0.8381105749526867 1104 68-69 0.2737043335976317 1104 70-71 0.5240399244684717 1104 72-73 0.4204378996276148 1104 74-75 0.4249176137613979 1104 76-77 0.041667592641893236 1104 78-79 0.010351599129389655 1104 80-81 0.07335830052363335 1104 82-83 -0.03610295381819384 1104 84-85 0.12546688587632815 1104 86-87 -0.18613807021869277 1104 88-89 0.04622144524887162 1104 90-91 0.14038674145770358 1104 92-93 -0.0057926849344625 1104 94-95 -0.19310718481478162 1104 96-97 0.10064262809835967 1104 98-99 -0.06581074329602998 1104 100 0.29312904499242265 1105 1 0.051452139778863426 1105 2 -0.03984551124371194 1105 3 0.16507146768429948 1105 4 0.06472347015446545 1105 5 0.007581863166109315 1105 6 0.3302044856519757 1105 7 0.09852156758785924 1105 8 -0.07441853115472696 1105 9 -0.3382335358651787 1105 10-11 0.028709896264032864 1105 12-13 -0.12093191651619861 1105 14-15 0.005722336057019106 1105 16-17 -0.20429037020053897 1105 18-19 -0.22063863837229292 1105 20-21 -0.09500726457200415 1105 22-23 -0.06056310959486666 1105 24-25 0.0473666350813815 1105 26-27 -0.07294309680172972 1105 28-29 -0.08965464787797117 1105 30-31 -0.20690308347233355 1105 32-33 -0.20991084606528432 1105 34-35 0.11463957967830396 1105 36-37 -0.09756898942712411 1105 38-39 -0.1872706532687829 1105 40-41 -0.1687089243976132 1105 42-43 -0.4043752428340426 1105 44-45 -0.49786096941893376 1105 46-47 -0.727316521010092 1105 48-49 -0.5181440403990933 1105 50-51 -0.6951747978813181 1105 52-53 -0.6445532083036731 1105 54-55 -0.41278620938391697 1105 56-57 -0.48885452613801306 1105 58-59 -0.6919710219466246 1105 60-61 -0.6166828449996622 1105 62-63 -0.681219801593091 1105 64-65 -0.882273878316056 1105 66-67 -0.9411842925761711 1105 68-69 -0.8733245860027701 1105 70-71 -0.9010952086681705 1105 72-73 -0.8881601541594399 1105 74-75 -0.934812896380059 1105 76-77 -1.107279256223432 1105 78-79 -1.100396568901548 1105 80-81 -0.9398583117940014 1105 82-83 -1.1280350803811885 1105 84-85 -1.327934365260564 1105 86-87 -1.175576056864358 1105 88-89 -0.7114669092694932 1105 90-91 -1.454995809175628 1105 92-93 -0.4104951702010702 1105 94-95 -1.267714171068544 1105 96-97 -0.9690710966958882 1105 98-99 -1.1001837329364363 1105 100 -1.2598742011606276 1106 1 -0.09106281067728972 1106 2 -0.0670455199739024 1106 3 0.2614346146280049 1106 4 0.018882679780958256 1106 5 0.31744007027539567 1106 6 0.20251995118953658 1106 7 0.16487649359947198 1106 8 0.031611486160159075 1106 9 0.1077065604579559 1106 10-11 -0.07823224437939302 1106 12-13 0.07651140997848671 1106 14-15 0.3012421099450151 1106 16-17 -0.20760602489228575 1106 18-19 -0.17606736361855724 1106 20-21 -0.06513909049194666 1106 22-23 0.08462819076743955 1106 24-25 -0.16174877807502241 1106 26-27 -0.01918511231237119 1106 28-29 0.047677659657644256 1106 30-31 0.14217369857838946 1106 32-33 0.3849627116340173 1106 34-35 0.15416098320238802 1106 36-37 0.207992325631011 1106 38-39 -0.06984318662539835 1106 40-41 0.19817563334534327 1106 42-43 0.245222986391731 1106 44-45 0.07828177651789048 1106 46-47 0.10674341904258355 1106 48-49 -0.030218079714064316 1106 50-51 0.014704507195290262 1106 52-53 0.1086214901408944 1106 54-55 0.3297158276612038 1106 56-57 0.24394244593991843 1106 58-59 0.009715542285771761 1106 60-61 -0.01287084243880443 1106 62-63 0.36034046584183343 1106 64-65 0.3022714968640017 1106 66-67 0.10284043569656376 1106 68-69 0.15295693363288976 1106 70-71 0.15180521620123955 1106 72-73 0.2824487404525762 1106 74-75 0.018794310394572022 1106 76-77 0.040830293153085506 1106 78-79 -0.2537833382934913 1106 80-81 -0.13520639187897743 1106 82-83 -0.34509951648345094 1106 84-85 -0.26187226448503154 1106 86-87 -0.6690174990923055 1106 88-89 -0.38900903245251683 1106 90-91 -0.3701456047436018 1106 92-93 -0.7585579766672339 1106 94-95 -0.7233711544957266 1106 96-97 -0.6631287450728003 1106 98-99 -0.7340748892505502 1106 100 -0.5041122735339307 1107 1 -0.21176457252936487 1107 2 -0.1967054910352708 1107 3 0.15984440836931313 1107 4 0.19691201561095895 1107 5 0.06962819976159196 1107 6 0.23338428006859147 1107 7 -0.1191583178738469 1107 8 0.15825701627619537 1107 9 0.012375752162213871 1107 10-11 -0.15618036762391796 1107 12-13 -0.22421453832917848 1107 14-15 0.04621508636854088 1107 16-17 -0.18948036880808417 1107 18-19 -0.24991052757577137 1107 20-21 -0.14969947861008137 1107 22-23 0.18057784605724692 1107 24-25 0.005531415984044941 1107 26-27 -0.06491595465357136 1107 28-29 -0.09524053196150106 1107 30-31 0.03163237591262913 1107 32-33 -0.06396556594988567 1107 34-35 -0.07225367620124956 1107 36-37 -0.35761786814435226 1107 38-39 -0.2798947512975687 1107 40-41 -0.4603236715600403 1107 42-43 -0.21091335145262136 1107 44-45 -0.3139945164999567 1107 46-47 -0.38278583167378954 1107 48-49 -0.20737322766991895 1107 50-51 -0.1623637539333167 1107 52-53 -0.09194699795738259 1107 54-55 -0.17228256936549258 1107 56-57 -0.44655426299985024 1107 58-59 -0.5137279422661045 1107 60-61 -0.42011649455104205 1107 62-63 -0.5705348568806663 1107 64-65 -0.26677693278958614 1107 66-67 -0.3846319023747604 1107 68-69 -0.3109194246387368 1107 70-71 0.02695239184739151 1107 72-73 -0.35102893140602376 1107 74-75 -0.3050789979720996 1107 76-77 -0.16316933802119848 1107 78-79 -0.4674876961111778 1107 80-81 -0.2507924701267683 1107 82-83 -0.6749804283341021 1107 84-85 -0.30963519466348544 1107 86-87 -0.21206371579159722 1107 88-89 -0.266674152888303 1107 90-91 -0.27559145829142295 1107 92-93 -0.10105343727380856 1107 94-95 -0.3511355741626474 1107 96-97 -0.680733080193523 1107 98-99 -0.3659318558008948 1107 100 -0.43178696925271254 1108 1 0.25674583651068517 1108 2 0.4298088965097975 1108 3 0.560975795734798 1108 4 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0.11629240554344378 2316 100 0.2643323128601942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 39.0 1 17.0 2 21.0 3 27.0 4 12.0 5 30.0 6 32.0 7 62.0 8 90.0 9 115.0 10 144.0 11 137.0 12 229.0 13 712.0 14 2457.0 15 5977.0 16 10709.0 17 16953.0 18 24969.0 19 31478.0 20 37181.0 21 44262.0 22 47689.0 23 36640.0 24 24338.0 25 19354.0 26 15823.0 27 15017.0 28 15548.0 29 15570.0 30 16143.0 31 19608.0 32 24549.0 33 32371.0 34 46043.0 35 75061.0 36 134320.0 37 32972.0 38 4024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.27644551025846 16.446842525979218 14.664268585131895 14.612443378630429 2 16.86105169162285 23.609284100677716 36.875045814091415 22.65461839360802 3 25.325449309370168 31.396725507972906 30.232120953549146 13.04570422910778 4 12.59610037513319 19.011638063816648 35.15970165375993 33.232559907290224 5 9.553078362314182 39.58829812912492 32.05025451315774 18.80836899540316 6 23.817369639722145 33.57754431989506 17.128361400267405 25.47672464011539 7 18.733996339735466 40.258111298167606 21.054252158369756 19.953640203727176 8 26.824629009318592 25.864245335707746 21.32105073038478 25.990074924588885 9 28.54265690270606 17.540606897507736 21.49759677544046 32.419139424345744 10-11 23.64746602882927 26.821017985815228 22.731885095992084 26.799630889363417 12-13 31.01750794907263 26.657178419034068 24.912846714161212 17.412466917732093 14-15 27.825754365203114 25.40320323682043 30.669966389735144 16.10107600824131 16-17 16.77794341379156 30.748634058214876 31.83598969221245 20.637432835781112 18-19 17.027620318254094 28.585090119568534 36.69685091319119 17.690438648986177 20-21 20.376831123755675 25.44156565121395 35.18823229122307 18.993370933807306 22-23 18.039988457819394 26.90560935316474 34.51418548657422 20.54021670244165 24-25 19.235959279060516 27.772576338434003 33.509079047587946 19.48238533491753 26-27 19.03932720917757 24.892860593823197 36.658236789582936 19.409575407416295 28-29 17.336985965443045 27.9681578619441 37.232924600157396 17.46193157245546 30-31 18.57380651885061 28.09833317118613 35.31939222786258 18.00846808210068 32-33 15.292037261477306 29.100518171999525 37.43990917165105 18.167535394872118 34-35 15.529483565385002 28.326183117050117 36.36556249737655 19.778770820188328 36-37 16.77159699293003 28.303473160488103 36.69743290455138 18.22749694203049 38-39 17.906200824253794 27.01736316067886 36.383290006489034 18.693146008578314 40-41 15.51604088695454 29.152110744719057 35.89815174494493 19.43369662338147 42-43 16.210104548426372 29.77594099915159 34.37928412286156 19.63467032956047 44-45 17.012379268308127 28.4021325960738 34.272703823356316 20.312784312261762 46-47 16.750513110908617 29.700039262400153 33.94551125444368 19.60393637224755 48-49 17.334663059695025 28.935681153662056 33.25751404279035 20.47214174385257 50-51 16.089915922133482 29.279802047385704 33.48822823211171 21.142053798369105 52-53 16.694638812719038 29.53758643259824 30.72168136815121 23.04609338653151 54-55 16.479525054849553 27.63624415848922 31.025853267113995 24.858377519547233 56-57 18.697718802206232 26.144913667928556 30.11716852396181 25.040199005903407 58-59 19.211130151132764 25.031846879139096 30.9041315662535 24.852891403474644 60-61 17.33544827898629 25.158970676114194 32.60882454633816 24.896756498561352 62-63 16.117376437177867 24.816020581979174 33.18531042508955 25.88129255575341 64-65 15.587662739987767 25.924541676493355 32.71841568433073 25.76937989918815 66-67 16.921030836200135 24.322611725338668 31.495514728468333 27.260842709992865 68-69 16.57113996383267 24.32659338140541 32.4209989059183 26.681267748843617 70-71 17.266884992487416 24.112898658395604 32.3257696651486 26.294446683968374 72-73 17.131228038381945 22.227789200447194 34.18450357582113 26.45647918534973 74-75 16.930460621496827 23.632966214248537 32.79928135556173 26.637291808692904 76-77 17.569905140636536 21.567245133759965 33.76956024595735 27.09328947964615 78-79 18.20015069361031 21.292500827748942 34.64933756767722 25.858010910963525 80-81 18.716364162007583 21.466917223614082 33.82192382171732 25.99479479266102 82-83 17.948217495047302 21.138127980111935 33.69819651186183 27.21545801297893 84-85 18.1430740136542 21.80988434096733 32.374404069215565 27.672637576162902 86-87 19.34168988733507 21.36130516254912 31.3481354963472 27.94886945376861 88-89 19.931787903010925 20.659872102318143 30.854649613642422 28.553690381028513 90-91 20.9440306238354 19.85199723127698 30.926156504800296 28.277815640087322 92-93 21.051085105419 20.652449116111477 30.884328268708504 27.41213750976102 94-95 20.14549587723927 20.16395102789193 31.102991733158508 28.587561361710296 96-97 20.139509783673223 19.372738962137817 31.38310559171736 29.1046456624716 98-99 21.46685215018302 19.506016888060966 30.147667264957605 28.879463696798414 100 21.049966486461997 18.22896609069576 31.435565392708043 29.285502030134204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9800.0 1 5529.5 2 1279.0 3 1152.0 4 766.5 5 703.0 6 1291.5 7 2445.5 8 3253.5 9 2846.0 10 1701.5 11 883.5 12 701.5 13 679.5 14 733.0 15 792.5 16 877.5 17 1033.0 18 1290.5 19 1648.5 20 2033.5 21 2684.5 22 3666.0 23 4824.5 24 6450.0 25 8651.0 26 10911.0 27 13498.0 28 16271.0 29 18561.5 30 20221.5 31 20925.5 32 20672.5 33 19447.5 34 17222.5 35 14466.5 36 11873.0 37 9696.0 38 7803.0 39 6422.5 40 5502.0 41 4789.5 42 4612.5 43 4849.0 44 5459.0 45 7037.0 46 12829.0 47 28650.5 48 60067.5 49 74105.5 50 70018.0 51 59155.5 52 35302.5 53 19002.0 54 11069.5 55 7549.0 56 6218.0 57 5836.0 58 5557.0 59 5236.0 60 5078.0 61 4933.0 62 4641.0 63 4269.0 64 3922.5 65 3514.0 66 3113.5 67 2819.0 68 2506.5 69 2218.0 70 1979.5 71 1705.0 72 1387.5 73 1151.5 74 924.5 75 732.0 76 599.0 77 467.0 78 335.0 79 232.5 80 181.5 81 130.0 82 80.0 83 55.0 84 39.0 85 26.5 86 15.5 87 5.5 88 4.5 89 2.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016384205625776752 2 0.05434760890501557 3 0.09097896294638634 4 0.11402341476150324 5 0.11455623445665046 6 0.07352911793031518 7 0.06713528158854865 8 0.06686887174097504 9 0.06407156834145217 10-11 0.03603193188433017 12-13 0.042758780535563715 14-15 0.04755415779188862 16-17 0.05547985075720339 18-19 0.05887657631376686 20-21 0.06214009694654354 22-23 0.05747792461400543 24-25 0.05281575228146733 26-27 0.055346645833416586 28-29 0.05281575228146733 30-31 0.05008505134383788 32-33 0.039495259902787044 34-35 0.0372307761984114 36-37 0.029971107852030644 38-39 0.030104312775817446 40-41 0.04162653868337589 42-43 0.06613624466014761 44-45 0.07426174501114259 46-47 0.08545095860923403 48-49 0.09244421710804118 50-51 0.08585057338059443 52-53 0.07619321640605123 54-55 0.0651372077317466 56-57 0.052416137510106924 58-59 0.03423366541320833 60-61 0.025508742905172743 62-63 0.021779005039142266 64-65 0.028772263537949417 66-67 0.04295858792124392 68-69 0.04289198545935052 70-71 0.0420261534547363 72-73 0.035432509727289556 74-75 0.0561458753761374 76-77 0.046488518401594195 78-79 0.02577515275274635 80-81 0.017116832706604166 82-83 0.01625100070198995 84-85 0.014852349002228519 86-87 0.017916062249324984 88-89 0.016384205625776752 90-91 0.02617476752410676 92-93 0.03849622297438603 94-95 0.03396725556563473 96-97 0.03170277186125908 98-99 0.03356764079427432 100 0.0372973786603048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 750723.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.705752044852666 #Duplication Level Percentage of deduplicated Percentage of total 1 87.75729722915787 34.84469483907378 2 5.548568785326849 4.406201927879945 3 2.0064400233227855 2.3900163017676874 4 1.0729986159964777 1.7041686796490492 5 0.6785680223775972 1.3471526821045454 6 0.47244770732149693 1.125533491263989 7 0.33748797053508794 0.9380149573330718 8 0.24794770357346357 0.7875960030542858 9 0.1945469303406503 0.6952168959473776 >10 1.3938456607532799 10.670990313700411 >50 0.15362791046626656 4.232461618897398 >100 0.10074509997366429 8.020652957572132 >500 0.016735066440808022 4.871158244111294 >1k 0.01573096245435954 13.287042953142667 >5k 0.0020082079728969628 5.676506063157358 >10k+ 0.0010041039864484814 5.00259207134505 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13663 1.8199788736990872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12446 1.6578684814505484 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11534 1.5363855909569841 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7909 1.0535177422298239 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7692 1.0246122737680876 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7330 0.9763920913572649 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6769 0.9016641291128686 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6604 0.8796853166880461 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6410 0.8538435614734062 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4623 0.6158063626663897 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4059 0.5406787856506329 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3486 0.4643523643207948 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 3346 0.44570367499064234 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3341 0.44503765037170834 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3286 0.43771137956343414 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3274 0.43611292047799255 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3167 0.42185999363280463 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3105 0.41360128835802284 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3041 0.4050761732356675 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3032 0.4038773289215863 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2819 0.3755046801549972 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2815 0.37497186045985004 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2784 0.37084250782245914 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2701 0.35978649914815447 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2679 0.35685599082484487 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2659 0.3541918923491088 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2515 0.33501038332380917 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2341 0.31183272658490546 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2221 0.2958481357304892 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2159 0.28758943045570734 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2125 0.2830604630469561 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2046 0.27253727406779865 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2005 0.2670758721925397 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1910 0.25442140443279343 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1833 0.24416462530120966 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1694 0.22564914089484406 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 1629 0.21699082084870183 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1615 0.2151259519156866 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1555 0.20713365648847842 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1483 0.19754290197582866 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 1427 0.19008342624376767 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1333 0.17756216340780823 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1289 0.17170114676118886 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1262 0.1681046138189452 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1237 0.16477449072427514 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1195 0.15917988392522942 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1192 0.158780269153869 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1140 0.15185361311695525 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1135 0.15118758849802125 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1114 0.14839028509849836 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1108 0.14759105555577756 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1095 0.14585939154654912 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 1063 0.14159683398537143 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 1019 0.13573581733875212 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1012 0.13480338287224447 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1012 0.13480338287224447 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 995 0.13253889916786885 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 987 0.1314732597775744 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 979 0.13040762038728 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 966 0.12867595637805154 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 964 0.12840954653047795 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 958 0.12761031698775716 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.12681108744503633 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 939 0.12507942343580788 No Hit GTACATGGGCTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAA 933 0.12428019389308707 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 925 0.12321455450279264 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 917 0.12214891511249822 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 917 0.12214891511249822 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 909 0.12108327572220379 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 888 0.11828597232268093 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 866 0.11535546399937127 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 841 0.1120253409047012 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 788 0.10496547994400066 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 786 0.10469907009642704 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 780 0.10389984055370623 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 775 0.10323381593477221 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 769 0.1024345863920514 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 766 0.10203497162069097 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 765 0.10190176669690419 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 765 0.10190176669690419 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 761 0.10136894700175697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.9961477136040856E-4 0.0 2 0.0 0.0 0.0 5.328196951472114E-4 0.0 3 0.0 0.0 0.0 5.328196951472114E-4 0.0 4 0.0 0.0 0.0 5.328196951472114E-4 0.0 5 0.0 0.0 0.0 5.328196951472114E-4 0.0 6 0.0 0.0 0.0 5.328196951472114E-4 0.0 7 0.0 0.0 0.0 5.328196951472114E-4 0.0 8 0.0 0.0 0.0 6.660246189340143E-4 0.0 9 0.0 0.0 0.0 7.992295427208171E-4 0.0 10-11 0.0 0.0 0.0 0.0011322418521878242 0.0 12-13 0.0 0.0 0.0 0.0013986516997614301 0.0 14-15 0.0 0.0 0.0 0.0015984590854416342 0.0 16-17 0.0 0.0 0.0 0.002197881242482247 6.660246189340143E-5 18-19 0.0 0.0 0.0 0.0035965329422436767 1.3320492378680285E-4 20-21 0.0 0.0 0.0 0.017916062249324984 1.3320492378680285E-4 22-23 1.3320492378680285E-4 0.0 0.0 0.06660246189340144 1.3320492378680285E-4 24-25 1.3320492378680285E-4 0.0 0.0 0.15724841253032076 1.3320492378680285E-4 26-27 1.3320492378680285E-4 0.0 0.0 0.3174939358458446 1.3320492378680285E-4 28-29 1.3320492378680285E-4 0.0 0.0 0.7556715326425325 1.3320492378680285E-4 30-31 1.3320492378680285E-4 0.0 0.0 1.4831702239041564 1.3320492378680285E-4 32-33 1.3320492378680285E-4 0.0 0.0 2.4760131233490914 1.3320492378680285E-4 34-35 1.3320492378680285E-4 0.0 0.0 4.012798329077436 1.3320492378680285E-4 36-37 2.664098475736057E-4 0.0 0.0 5.8215213867165385 1.3320492378680285E-4 38-39 2.664098475736057E-4 0.0 0.0 7.658750298046018 1.3320492378680285E-4 40-41 2.664098475736057E-4 0.0 0.0 9.496112414299283 1.3320492378680285E-4 42-43 2.664098475736057E-4 0.0 0.0 10.94777967372786 1.3320492378680285E-4 44-45 3.9961477136040856E-4 0.0 0.0 12.30353938802994 1.3320492378680285E-4 46-47 3.9961477136040856E-4 0.0 0.0 13.849249323652 1.3320492378680285E-4 48-49 3.9961477136040856E-4 0.0 0.0 15.283599942988292 1.3320492378680285E-4 50-51 6.660246189340143E-4 0.0 0.0 16.42589876692202 1.3320492378680285E-4 52-53 6.660246189340143E-4 0.0 0.0 17.315174838122715 1.3320492378680285E-4 54-55 6.660246189340143E-4 0.0 0.0 18.262195243784994 1.3320492378680285E-4 56-57 6.660246189340143E-4 0.0 0.0 19.302792108407495 1.3320492378680285E-4 58-59 6.660246189340143E-4 0.0 0.0 20.095161597553293 1.3320492378680285E-4 60-61 6.660246189340143E-4 0.0 0.0 20.82172785434841 1.3320492378680285E-4 62-63 6.660246189340143E-4 0.0 0.0 21.44133055734272 1.3320492378680285E-4 64-65 6.660246189340143E-4 0.0 0.0 22.028631066318738 1.3320492378680285E-4 66-67 6.660246189340143E-4 0.0 0.0 22.657091896744873 1.3320492378680285E-4 68-69 6.660246189340143E-4 0.0 0.0 23.38725468648223 1.3320492378680285E-4 70-71 6.660246189340143E-4 0.0 0.0 24.180290200246965 1.3320492378680285E-4 72-73 6.660246189340143E-4 0.0 0.0 24.835125605582885 1.3320492378680285E-4 74-75 6.660246189340143E-4 0.0 0.0 25.469913669888893 1.3320492378680285E-4 76-77 6.660246189340143E-4 0.0 0.0 26.03123921872648 1.3320492378680285E-4 78-79 6.660246189340143E-4 0.0 0.0 26.69733044012239 1.3320492378680285E-4 80-81 6.660246189340143E-4 0.0 0.0 27.302813421195303 1.3320492378680285E-4 82-83 6.660246189340143E-4 0.0 0.0 27.88431951598659 1.3320492378680285E-4 84-85 6.660246189340143E-4 6.660246189340143E-5 0.0 28.52063943691615 1.3320492378680285E-4 86-87 6.660246189340143E-4 1.3320492378680285E-4 0.0 29.105342449878318 1.3320492378680285E-4 88 6.660246189340143E-4 2.664098475736057E-4 0.0 29.548448628855116 1.3320492378680285E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 670 0.0 66.67604 1 TATAACG 55 1.2803866E-8 59.848923 2 ACCCATG 865 0.0 51.645023 3 TACCCAT 895 0.0 50.964725 2 TAACGCA 100 1.2732926E-11 47.02415 4 ATAACGC 85 7.887138E-9 44.25803 3 GTATAAC 85 3.9373117E-7 38.725777 1 GTACCGG 55 0.0027095738 34.199387 1 ACTGGGA 130 9.133146E-9 32.555183 3 TACAACG 60 0.004149619 31.349434 2 AGTAAAT 350 0.0 30.901587 2 GAGTAGG 110 2.9547737E-6 29.924461 1 CAAGCCA 110 2.9640069E-6 29.912487 6 AGAGAGC 65 0.006153419 28.9225 8 AAAAGTA 180 2.3646862E-10 28.736982 2 AAGTACT 215 1.8189894E-12 28.43321 4 GTAAATG 385 0.0 28.09235 3 AAGCCAC 120 5.8344776E-6 27.419777 7 AGTAGGG 70 0.00881199 26.870945 2 TAAATGG 405 0.0 26.705074 4 >>END_MODULE