##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522866_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 750723 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.15354931179676 34.0 33.0 35.0 29.0 36.0 2 31.785372234499278 33.0 31.0 34.0 30.0 34.0 3 32.8362871525183 34.0 31.0 35.0 30.0 35.0 4 33.764018153167015 35.0 32.0 36.0 30.0 36.0 5 34.56764878656975 36.0 35.0 36.0 31.0 36.0 6 34.84553423832759 36.0 35.0 37.0 31.0 37.0 7 34.948375099737184 36.0 35.0 36.0 32.0 38.0 8 34.84554622677073 36.0 35.0 37.0 31.0 37.0 9 34.99144957594213 36.0 35.0 36.0 32.0 37.0 10-11 34.967219600305306 36.0 34.5 36.5 31.5 37.5 12-13 34.951241669697076 36.0 35.0 37.0 31.5 37.0 14-15 35.53044065520838 37.0 35.5 37.5 31.5 38.0 16-17 35.64073433210385 37.0 36.0 37.0 31.5 37.5 18-19 35.65191555340651 36.5 35.5 37.5 31.5 39.0 20-21 35.73957771375061 37.0 36.0 37.5 31.0 38.0 22-23 35.781681126061144 37.0 36.0 37.5 31.5 37.5 24-25 35.803085825264446 36.5 36.0 37.5 32.0 38.5 26-27 35.71705808933521 37.0 36.5 37.0 32.0 38.0 28-29 35.43254036442203 37.0 35.0 37.0 31.5 38.0 30-31 35.31155233022034 37.0 35.0 37.0 31.0 37.5 32-33 35.3031124662492 37.0 35.5 37.0 31.0 38.5 34-35 35.03917956423341 37.0 36.0 37.0 30.0 38.5 36-37 34.62439608217678 36.0 35.0 37.0 28.5 38.0 38-39 34.29001509211787 37.0 35.0 37.5 27.5 38.5 40-41 34.30202284997263 37.0 35.5 37.5 27.5 38.0 42-43 33.660754366124394 36.0 34.5 37.5 26.5 38.0 44-45 33.017449178991455 36.5 34.0 37.0 20.0 38.0 46-47 32.13783712501149 36.0 32.5 37.0 13.5 37.5 48-49 31.46243954161522 36.5 30.5 37.0 12.5 38.0 50-51 30.895937649439276 36.5 27.5 37.0 10.0 38.0 52-53 30.45464971767216 36.5 27.5 37.0 8.0 37.5 54-55 30.42463398617066 36.5 27.0 37.0 8.0 37.5 56-57 30.094566837568586 36.0 26.0 37.0 8.0 38.5 58-59 29.634997196036352 36.0 25.0 37.0 8.0 38.0 60-61 29.057616457734746 35.5 24.0 37.0 8.0 37.0 62-63 29.08651060377796 36.5 23.5 37.0 7.5 37.0 64-65 28.62787805888457 36.5 18.5 37.0 7.0 37.5 66-67 28.152935237098106 35.5 13.0 37.0 7.5 37.5 68-69 27.406807837244898 35.0 13.0 37.0 7.0 37.5 70-71 26.808870249079888 35.0 12.0 37.0 7.0 37.5 72-73 26.41994384080413 33.5 11.5 37.0 6.0 37.5 74-75 26.283755126724504 35.5 12.5 36.5 7.0 37.0 76-77 26.312201038199177 34.5 12.0 36.5 7.5 37.0 78-79 26.26032105050731 33.5 12.0 37.0 7.0 37.0 80-81 26.216200915650646 33.0 12.0 36.5 7.5 37.5 82-83 26.184681966584215 34.5 12.5 37.0 7.0 37.0 84-85 26.365983192202716 33.5 12.0 36.5 7.0 37.0 86-87 26.2991656043574 33.5 12.0 37.0 7.0 38.0 88-89 26.11523824366644 33.0 12.5 37.0 6.5 37.5 90-91 26.068968181339855 33.5 12.0 36.5 7.0 38.0 92-93 26.131090295621686 33.5 11.5 37.0 7.0 37.5 94-95 25.990302015523703 34.0 11.5 37.0 7.0 37.0 96-97 25.796466872601478 33.0 11.5 36.5 7.0 37.0 98-99 25.95563077193585 33.0 12.5 37.0 7.5 37.5 100 25.86101398252085 32.0 11.0 37.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2357410948593639 1101 2 0.37219949136438046 1101 3 0.11313311009253368 1101 4 0.023655907529672504 1101 5 0.18411806189374147 1101 6 0.25719715074301064 1101 7 0.3264484064680886 1101 8 0.17353098108700493 1101 9 0.1979337929438998 1101 10-11 0.1614501797215624 1101 12-13 0.2972341085533685 1101 14-15 0.2290619602204984 1101 16-17 0.06909477014406207 1101 18-19 0.23842707038055266 1101 20-21 0.2584007850621859 1101 22-23 0.17459415384773536 1101 24-25 0.2159293439466623 1101 26-27 0.22739279601470486 1101 28-29 0.1660297423693251 1101 30-31 0.32352889414664077 1101 32-33 0.31198541967906124 1101 34-35 0.3350189447240979 1101 36-37 0.38908325141128586 1101 38-39 0.26046233467500457 1101 40-41 0.1769840044265365 1101 42-43 -0.02295449820097417 1101 44-45 0.05910856979846102 1101 46-47 0.12749840640339016 1101 48-49 0.07917423199551976 1101 50-51 -0.05227632552636052 1101 52-53 -0.11991481825460326 1101 54-55 -0.826461468558854 1101 56-57 -0.2853620786177622 1101 58-59 -0.1957460480065869 1101 60-61 -0.31720990120807 1101 62-63 -0.48407264675509865 1101 64-65 -0.7829832121076059 1101 66-67 -0.3297390534186917 1101 68-69 -0.27926262859352846 1101 70-71 -0.13955358251560668 1101 72-73 -0.03863996568549766 1101 74-75 -0.5220950593749656 1101 76-77 -0.37738665520889825 1101 78-79 -0.2874983018971129 1101 80-81 -0.3004106915475724 1101 82-83 -0.4802323368006043 1101 84-85 -0.39550895609477976 1101 86-87 -0.035618918546209244 1101 88-89 -0.013285213493730907 1101 90-91 -0.3129069791761694 1101 92-93 -0.45092299908266753 1101 94-95 -0.1452553790329567 1101 96-97 -0.18972468421044297 1101 98-99 -0.08109140876893761 1101 100 -0.49005233968009776 1102 1 0.17849939931645764 1102 2 0.3305964298062456 1102 3 0.20235632484466493 1102 4 0.09781511087448536 1102 5 0.007153655602692766 1102 6 0.16196264862348642 1102 7 0.22006003512647254 1102 8 0.19485973291776304 1102 9 0.037664397497159996 1102 10-11 -0.6568471961686626 1102 12-13 0.23877061449984183 1102 14-15 0.006733326409865015 1102 16-17 -0.15622693143527755 1102 18-19 0.10484086441802276 1102 20-21 0.03157094106158098 1102 22-23 -0.14384155710743585 1102 24-25 -0.077614761721982 1102 26-27 -0.0244360322389241 1102 28-29 -0.20620863506677267 1102 30-31 0.06105793967338258 1102 32-33 -0.10293174373624936 1102 34-35 -0.19534705309736466 1102 36-37 -0.18385995769135377 1102 38-39 -0.42355522517183886 1102 40-41 -0.06055761701520623 1102 42-43 -0.03100598593172066 1102 44-45 -0.16652559489958918 1102 46-47 -0.13792401858127334 1102 48-49 -0.06752671855018022 1102 50-51 0.08573563513617799 1102 52-53 -0.11638098614113446 1102 54-55 -0.32377796177463125 1102 56-57 -0.3212692180614418 1102 58-59 -0.22822963643751848 1102 60-61 -0.19438867848087682 1102 62-63 -0.007925287987458063 1102 64-65 -0.888339391041594 1102 66-67 -0.42961697030543533 1102 68-69 -0.19128152729162906 1102 70-71 -0.3994184204507576 1102 72-73 -0.3286134788737627 1102 74-75 -0.5472304439367726 1102 76-77 -0.17499278142078012 1102 78-79 -0.20258378909773356 1102 80-81 -0.5354390647465586 1102 82-83 -0.6284075715348969 1102 84-85 -0.5801998110828706 1102 86-87 -0.44410882202122437 1102 88-89 -0.9921431656317381 1102 90-91 -0.9884890021959016 1102 92-93 -0.7255587328679596 1102 94-95 -0.6507945858990212 1102 96-97 -1.0860739928253302 1102 98-99 -0.47127078001188494 1102 100 -0.6709443009617573 1103 1 0.24955830063082374 1103 2 0.2722498920301426 1103 3 0.12515308282147686 1103 4 0.08963975587446527 1103 5 0.023793153415880397 1103 6 0.26886353740598423 1103 7 0.10260889156472786 1103 8 0.2894681404429633 1103 9 0.02550491062750382 1103 10-11 0.16885985899360634 1103 12-13 0.2933173126291777 1103 14-15 0.11992009992801655 1103 16-17 -0.04969842693817128 1103 18-19 0.11221540321248114 1103 20-21 0.19481585212633235 1103 22-23 0.1760612429944004 1103 24-25 0.03335195548255854 1103 26-27 0.11672231087899831 1103 28-29 0.005073848812408244 1103 30-31 0.12474515207302517 1103 32-33 0.0567451179001921 1103 34-35 -0.05921779775194125 1103 36-37 -0.3585471358924366 1103 38-39 -0.4021716275596674 1103 40-41 -0.5106324738562407 1103 42-43 -0.46760851130514425 1103 44-45 -0.5165725229192262 1103 46-47 -0.1443096491076652 1103 48-49 -0.6841418973896332 1103 50-51 -0.7048428524516481 1103 52-53 -0.7187382166660079 1103 54-55 -0.4188138512600972 1103 56-57 -0.33780612020417067 1103 58-59 -0.4468781094719567 1103 60-61 -0.1552655294777665 1103 62-63 -0.3761468490375819 1103 64-65 -0.4056951586282018 1103 66-67 -0.793419317846368 1103 68-69 -0.6362019016518303 1103 70-71 -0.36841362885295936 1103 72-73 -0.3555429424503984 1103 74-75 -0.14001112859188325 1103 76-77 -0.3125260775156846 1103 78-79 -0.21951112476416768 1103 80-81 -0.6147018198582934 1103 82-83 0.0493897754170618 1103 84-85 -0.4868396508816346 1103 86-87 -0.1553390581052625 1103 88-89 -0.3766603390070209 1103 90-91 -0.15213764622312098 1103 92-93 -0.4944287365538038 1103 94-95 0.0222311124165131 1103 96-97 0.10468650708157057 1103 98-99 -0.3513981886352404 1103 100 -0.23120975775947983 1104 1 0.3260572486047266 1104 2 0.3888280068599279 1104 3 -0.028669783230625967 1104 4 -0.18580943656306204 1104 5 0.0936015181586285 1104 6 0.3230580184316665 1104 7 0.2917616699732193 1104 8 0.30396944979919027 1104 9 0.2492837278171578 1104 10-11 0.18478405256640684 1104 12-13 0.32266051532963047 1104 14-15 0.24225976055499387 1104 16-17 -0.03937305570162408 1104 18-19 0.2738546717894508 1104 20-21 0.14796663244208474 1104 22-23 0.19438844581594594 1104 24-25 0.09835051746127732 1104 26-27 0.3224123340387095 1104 28-29 0.1596895187032672 1104 30-31 0.2549598713561352 1104 32-33 0.13880126127848058 1104 34-35 0.18582859735576562 1104 36-37 0.02893715940145114 1104 38-39 0.1043883984760896 1104 40-41 0.1953678591592123 1104 42-43 0.4664922712811759 1104 44-45 0.43324053431402376 1104 46-47 0.5895996495920599 1104 48-49 0.72932895618953 1104 50-51 0.6740246679455488 1104 52-53 0.5258332529917418 1104 54-55 0.3515436105566536 1104 56-57 0.40254400013550296 1104 58-59 0.6015113066913997 1104 60-61 0.33708268359157856 1104 62-63 0.1907608754072676 1104 64-65 0.2118933553660156 1104 66-67 0.1768420236004289 1104 68-69 0.32641900566391513 1104 70-71 0.704083457261806 1104 72-73 0.4961970633016861 1104 74-75 0.36581853400291564 1104 76-77 0.4755531044772603 1104 78-79 0.5049034492031055 1104 80-81 0.11340619824563802 1104 82-83 0.14733226970282232 1104 84-85 -0.5464796914489725 1104 86-87 0.713585301975268 1104 88-89 0.2573424310760828 1104 90-91 0.016773090497888887 1104 92-93 0.3560908432412404 1104 94-95 0.2979224979121753 1104 96-97 0.6003648747003609 1104 98-99 0.48566832363335166 1104 100 0.3199875841717059 1105 1 0.14562770639364686 1105 2 0.285605515973554 1105 3 0.009703590919940552 1105 4 0.24840974901485424 1105 5 0.19289941740218097 1105 6 0.20355854713313448 1105 7 0.19879524806205495 1105 8 0.2213293439110089 1105 9 0.020999308612353218 1105 10-11 0.23072192459846974 1105 12-13 0.19482567723059674 1105 14-15 0.07599516580651766 1105 16-17 0.0340619140182028 1105 18-19 0.18656439619919496 1105 20-21 0.1369008738109443 1105 22-23 0.12086012146684055 1105 24-25 0.06878390477391605 1105 26-27 0.3578057544783846 1105 28-29 0.15819122311848588 1105 30-31 0.25247072267640647 1105 32-33 0.16859987408414412 1105 34-35 0.2393420589084485 1105 36-37 0.1258716152086663 1105 38-39 0.05819819165399309 1105 40-41 0.01899785686693889 1105 42-43 0.29226295533953106 1105 44-45 -0.17361261977842446 1105 46-47 0.028730582694691975 1105 48-49 -0.23059233014483382 1105 50-51 -0.590278925006082 1105 52-53 -0.9066343779080803 1105 54-55 -0.6431943309830146 1105 56-57 -0.7079120880652106 1105 58-59 -0.7942180425251593 1105 60-61 -0.399679445115801 1105 62-63 -0.7531292951821449 1105 64-65 -0.8884730384463282 1105 66-67 -0.4396395975535441 1105 68-69 -0.7945436376955222 1105 70-71 -0.9097324732897434 1105 72-73 -0.9250892710594734 1105 74-75 -0.69765059796541 1105 76-77 -0.7676874657808774 1105 78-79 -1.1462885322812504 1105 80-81 -0.5243667485070986 1105 82-83 -1.0343996158018882 1105 84-85 -1.0405826366488782 1105 86-87 -0.9150961979101204 1105 88-89 -0.7130567570159343 1105 90-91 -1.0466673880693698 1105 92-93 -1.1160977959625278 1105 94-95 -1.2921527034010722 1105 96-97 -0.5728883769985096 1105 98-99 -0.7575611549856696 1105 100 -0.7969608101286703 1106 1 0.2874533353313282 1106 2 0.28910123090364337 1106 3 -0.043014045525708866 1106 4 0.03993091245862246 1106 5 0.20258265688593013 1106 6 0.3764909463357782 1106 7 0.1816567871951449 1106 8 0.28134032940433684 1106 9 0.07869497407133963 1106 10-11 0.24889254895280288 1106 12-13 0.19715372879693405 1106 14-15 0.09749508577974808 1106 16-17 0.092677887362008 1106 18-19 0.22257007810004126 1106 20-21 0.21706587446571035 1106 22-23 0.24583829598341111 1106 24-25 0.06624669232466118 1106 26-27 0.19997931799780844 1106 28-29 0.18584411062993667 1106 30-31 0.3375109906933389 1106 32-33 -0.1422559394971259 1106 34-35 0.4143342381102144 1106 36-37 0.2836634946737888 1106 38-39 0.1305760416983759 1106 40-41 0.09628007491806301 1106 42-43 0.005074150357494034 1106 44-45 0.08299595472650623 1106 46-47 0.1473402630241054 1106 48-49 0.40692458460482683 1106 50-51 0.2425069524511052 1106 52-53 0.024081534324370324 1106 54-55 0.3673200956615368 1106 56-57 0.2978925811332154 1106 58-59 0.2682110246536773 1106 60-61 0.15599463494007182 1106 62-63 0.5010684727984192 1106 64-65 0.25662277542594936 1106 66-67 0.299065714769128 1106 68-69 0.3917614170864425 1106 70-71 0.33622253711373773 1106 72-73 0.23026633881728742 1106 74-75 0.24948899055147677 1106 76-77 0.5138485389916276 1106 78-79 0.03720558211338343 1106 80-81 0.3170792988978519 1106 82-83 0.32348465820978234 1106 84-85 0.10181045485809648 1106 86-87 0.11995098150990557 1106 88-89 0.4085521084954351 1106 90-91 0.3089024753031069 1106 92-93 0.09881955820686628 1106 94-95 -0.011944415537499253 1106 96-97 0.3001709737661109 1106 98-99 0.18248878899559173 1106 100 0.05191954257114162 1107 1 0.3136612395501288 1107 2 0.3667177656441858 1107 3 0.2260053036387646 1107 4 0.3032760198436506 1107 5 0.4139765343790529 1107 6 0.48154598648103786 1107 7 0.3770883132824707 1107 8 0.4240510565584188 1107 9 0.34120667719501085 1107 10-11 0.37685911348311407 1107 12-13 0.3268598030727574 1107 14-15 0.46594842932671554 1107 16-17 0.15097362884355192 1107 18-19 0.43985630104100437 1107 20-21 0.36769571530322764 1107 22-23 0.43792911222308817 1107 24-25 0.33966357886819765 1107 26-27 0.34447211171075054 1107 28-29 0.4693788795464471 1107 30-31 0.4907633951533015 1107 32-33 0.14795138311189504 1107 34-35 0.28622581753541 1107 36-37 0.2892082928126385 1107 38-39 -0.11234711488232563 1107 40-41 -0.18114424488219782 1107 42-43 0.0023682463089258476 1107 44-45 -0.030494200464012522 1107 46-47 -0.24879806080486944 1107 48-49 -0.11187488769068565 1107 50-51 -0.3275451087172243 1107 52-53 -0.013731967533939127 1107 54-55 0.025046285124467715 1107 56-57 -0.011195780996896332 1107 58-59 -0.017041975601664205 1107 60-61 0.11720941917702987 1107 62-63 0.0097571555231859 1107 64-65 0.2626020077010338 1107 66-67 0.18116206339454166 1107 68-69 0.06828175924322011 1107 70-71 0.012251702716483237 1107 72-73 -0.16147267443206914 1107 74-75 0.19912122875614457 1107 76-77 0.06589906811467117 1107 78-79 -0.014791750992980468 1107 80-81 -0.2607305147033081 1107 82-83 -0.1535151573773561 1107 84-85 -0.34985209798328043 1107 86-87 0.009950485013749955 1107 88-89 0.002395201524631574 1107 90-91 0.0643710258180441 1107 92-93 -0.1370551261634354 1107 94-95 0.2053471270051297 1107 96-97 -0.13302901488540542 1107 98-99 -0.19316906841071102 1107 100 -0.4059917690298427 1108 1 0.3636791980797369 1108 2 0.32226004416716947 1108 3 0.2072041045615549 1108 4 0.14822483803425968 1108 5 0.34606911750632463 1108 6 0.4971560929889307 1108 7 0.46613153808181806 1108 8 0.42714199578951906 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94-95 -0.043570360390049245 2316 96-97 -0.3482906465894722 2316 98-99 -0.2510255301782607 2316 100 0.040940622098450064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 6.0 13 6.0 14 13.0 15 54.0 16 185.0 17 838.0 18 3050.0 19 7823.0 20 15264.0 21 23944.0 22 33823.0 23 41461.0 24 37270.0 25 27840.0 26 25026.0 27 25322.0 28 29748.0 29 35277.0 30 33564.0 31 25273.0 32 21745.0 33 26782.0 34 40574.0 35 83745.0 36 174209.0 37 37222.0 38 658.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.40600932509057 19.747971966079916 14.19383818915165 18.65218051967786 2 14.278654086190382 23.485021054047824 40.710042934005784 21.52628192575601 3 22.099525523429445 33.920412616121546 34.49266173097365 9.487400129475358 4 10.175018415629774 24.63072166623819 30.71007737894637 34.48418253918567 5 7.063953256012853 42.810554165634215 34.35645753901327 15.769035039339657 6 20.02895875043125 36.84341628003938 16.002706724051347 27.124918245478025 7 14.716684050464968 41.720583083233095 18.03266022733479 25.530072638967148 8 24.436774780511 29.775242733319036 22.77916829288111 23.008814193288853 9 24.001129577753712 17.74076456962155 18.647623690762106 39.610482161862635 10-11 21.53188218729561 27.997900673622684 23.661313117992535 26.80890402108917 12-13 29.674816241604656 25.48260722458886 24.524585927530325 20.317990606276158 14-15 26.50634988717528 25.67048787700374 26.908296280114342 20.91486595570664 16-17 17.64935858114593 33.350849885809396 27.96820645821303 21.031585074831643 18-19 18.039396950652574 28.906460215420438 32.450867036623606 20.60327579730338 20-21 19.520741366311363 25.668956018339678 30.74153947799585 24.06876313735311 22-23 16.96118275315929 31.59447625822041 30.073742245808372 21.37059874281193 24-25 20.036431692242115 33.457081202046034 29.166067242114234 17.340419863597614 26-27 19.984068585007954 31.347789715978568 32.36315101009966 16.30499068891381 28-29 17.051198285375943 32.54342340038243 33.259470760344925 17.1459075538967 30-31 21.516444623236602 31.499973691799767 31.65449274126784 15.32908894369579 32-33 15.902242919445117 32.843690973175015 34.762254268580215 16.491811838799656 34-35 15.395102808518802 29.02775483671588 35.0931643923934 20.48397796237192 36-37 14.238807123266502 33.56557611795562 35.017642992155565 17.17797376662231 38-39 16.450994352713316 26.387394941752156 36.78082679900282 20.38078390653171 40-41 13.720498859416889 29.016301180545153 37.40599763557954 19.85720232445843 42-43 17.38759769950347 28.726501333741837 35.98898372535242 17.896917241402278 44-45 14.610445185521165 29.955528736481956 35.34696177416164 20.087064303835238 46-47 17.899913616434322 27.981748224874337 32.83128297244717 21.287055186244167 48-49 17.339324950663734 26.98205911560371 32.479281432180365 23.199334501552187 50-51 13.701834379666373 27.844447374976188 35.47222188920725 22.98149635615019 52-53 14.783856777016654 28.781132716368006 35.07924085957767 21.35576964703767 54-55 13.952442861367384 27.475896408504923 31.59368764302974 26.97797308709795 56-57 19.27669678209991 28.16713232918955 26.85066719060638 25.70550369810416 58-59 15.653018897832883 30.49261976153591 31.66549224012649 22.188869100504714 60-61 16.6640691498927 34.67311916474517 26.396631486965127 22.266180198397 62-63 12.173852811084892 37.72620919642661 26.600423196270757 23.499514796217742 64-65 11.990571548268198 38.40735360623626 26.187398137621027 23.414676707874516 66-67 14.08928093309125 36.05118627857132 24.876616638404496 24.98291614993293 68-69 14.762432514156544 34.98980302725542 25.50168972696623 24.746074731621807 70-71 16.09678545167787 34.23676456483978 24.786868999030254 24.879580984452094 72-73 16.27401719967178 31.522141646863243 26.545066550155056 25.658774603309926 74-75 16.35057011935209 31.574222080136405 25.259217817561808 26.815989982949702 76-77 16.662559427313187 29.351198270430405 27.157066605213487 26.829175697042924 78-79 16.869931018222193 28.55573856858648 28.053544299329097 26.520786113862226 80-81 16.67376006980175 28.159744770696587 27.396454630526947 27.77004052897472 82-83 16.03832712464641 27.929433438724487 27.784967406891344 28.247272029737758 84-85 16.847870971523264 28.196273434232292 26.526405387908643 28.429450206335797 86-87 17.269288150042623 28.258538469735722 25.569986146632566 28.90218723358909 88-89 17.093275343592545 27.94603757122448 25.29014296513622 29.670544120046756 90-91 17.613487247463812 27.328528512526063 25.8314626949183 29.22652154509182 92-93 17.468216471741904 28.06416928476069 26.08574291249304 28.381871331004362 94-95 17.251855414239298 28.065480036395563 26.13692830527509 28.545736244090055 96-97 16.899267661545736 28.48739971559025 25.997382364597314 28.615950258266697 98-99 18.353995751843165 28.55383496396926 24.395796602673446 28.696372681514127 100 17.420841603282224 28.40164644136884 24.958106326011375 29.219405629337558 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9287.0 1 5259.5 2 1248.0 3 1105.5 4 732.5 5 706.0 6 1278.0 7 2379.5 8 3145.0 9 2798.5 10 1665.5 11 812.0 12 673.5 13 626.5 14 616.5 15 647.0 16 709.5 17 850.0 18 1019.0 19 1217.0 20 1537.0 21 1994.5 22 2552.0 23 3428.0 24 4705.5 25 6287.5 26 8365.0 27 11055.5 28 13983.0 29 16921.5 30 19449.5 31 21143.5 32 21867.0 33 21322.5 34 19626.0 35 17287.5 36 14985.0 37 13058.5 38 11442.0 39 10625.5 40 10570.0 41 10872.5 42 11760.5 43 12755.0 44 13604.5 45 14643.5 46 18719.0 47 32741.5 48 57805.0 49 72119.0 50 81371.0 51 73621.0 52 37594.0 53 12292.5 54 4482.0 55 2618.0 56 1881.5 57 1521.5 58 1216.0 59 954.5 60 839.0 61 723.0 62 592.5 63 490.5 64 366.0 65 241.0 66 124.0 67 84.5 68 62.0 69 51.0 70 44.5 71 33.5 72 24.5 73 24.5 74 27.5 75 27.0 76 19.5 77 14.0 78 12.0 79 6.0 80 3.0 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0651372077317466 2 0.004795377256324903 3 1.3320492378680285E-4 4 2.664098475736057E-4 5 0.01078959882673103 6 0.0 7 0.0031969181708832685 8 2.664098475736057E-4 9 0.0 10-11 7.992295427208171E-4 12-13 0.0011988443140812258 14-15 1.3320492378680285E-4 16-17 5.994221570406129E-4 18-19 6.660246189340143E-4 20-21 1.3320492378680285E-4 22-23 0.0 24-25 3.9961477136040856E-4 26-27 6.660246189340143E-4 28-29 5.994221570406129E-4 30-31 8.658320046142185E-4 32-33 6.660246189340143E-4 34-35 9.3243446650762E-4 36-37 0.0 38-39 0.0011322418521878244 40-41 0.0013986516997614301 42-43 0.0020646763186954443 44-45 0.0017982664711218386 46-47 1.9980738568020428E-4 48-49 9.990369284010214E-4 50-51 7.992295427208171E-4 52-53 7.326270808274157E-4 54-55 6.660246189340143E-4 56-57 6.660246189340143E-5 58-59 2.664098475736057E-4 60-61 7.992295427208171E-4 62-63 9.990369284010214E-4 64-65 0.002197881242482247 66-67 0.00186486893301524 68-69 0.0013320492378680285 70-71 0.0014652541616548315 72-73 0.0014652541616548315 74-75 3.9961477136040856E-4 76-77 0.0021312787805888456 78-79 0.00233108616626905 80-81 0.003396725556563473 82-83 0.0036631354041370785 84-85 6.660246189340143E-4 86-87 3.9961477136040856E-4 88-89 0.0020646763186954443 90-91 0.011722033293238652 92-93 0.023577271510264106 94-95 0.010922803750517834 96-97 0.0060608240322995305 98-99 0.005927619108512727 100 0.001731664009228437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 750723.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.75282004648503 #Duplication Level Percentage of deduplicated Percentage of total 1 79.84994598607938 20.563612897010515 2 9.143286857279266 4.709308421378092 3 3.3176146816828602 2.5631380164286623 4 1.761290126736061 1.8143275073393834 5 1.1017639436392426 1.4186764287123543 6 0.7493144567150306 1.1578176217207126 7 0.5815838063885657 1.0484196175512166 8 0.4312446865866083 0.8884613447734209 9 0.337214799040693 0.781580883303592 >10 2.2434889899454764 11.059243607602998 >50 0.250440509262881 4.50533738418169 >100 0.1716269328488879 9.024294934729252 >500 0.025925518557233823 4.810055223858678 >1k 0.02903658078410188 15.860550202084447 >5k 0.0031110622268680586 5.596419534927017 >10k+ 0.0031110622268680586 14.198756374398 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 40657 5.415712586400043 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 20726 2.760805250405276 RNA PCR Primer, Index 33 (95% over 22bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11594 1.5443778863841924 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11333 1.5096114012758368 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11138 1.4836364411374103 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 10885 1.449935595419349 RNA PCR Primer, Index 33 (95% over 24bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7714 1.0275427820913972 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7475 0.9957068053063514 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7177 0.9560117380178842 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6591 0.8779536526788176 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6510 0.8671640538520866 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6444 0.8583725288821576 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4840 0.6447118311281258 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 4434 0.5906306320706839 RNA PCR Primer, Index 33 (96% over 27bp) GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4148 0.5525340238676582 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 3363 0.44796815869501805 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3362 0.44783495377123117 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3361 0.4477017488474444 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3258 0.43398164169740366 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3108 0.4140009031293833 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3099 0.4128020588153021 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3033 0.40401053384537305 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3021 0.4024120747599314 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 2992 0.3985491319701141 RNA PCR Primer, Index 15 (95% over 24bp) TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2974 0.3961514433419517 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2964 0.3948193941040836 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2853 0.3800336475637486 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2819 0.3755046801549972 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2720 0.36231739270010377 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2716 0.3617845730049566 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2591 0.3451339575316062 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2483 0.33074782576263145 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2440 0.32502001403979897 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2245 0.2990450539013724 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2231 0.2971801849683572 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 2159 0.28758943045570734 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2149 0.28625738121783934 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2072 0.27600060208625554 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1961 0.2612148555459204 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1933 0.2574851176798899 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1910 0.25442140443279343 No Hit CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 1852 0.2466955188531589 No Hit TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT 1750 0.23310861662690496 RNA PCR Primer, Index 33 (95% over 21bp) TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 1650 0.2197881242482247 TruSeq Adapter, Index 1 (96% over 28bp) GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1616 0.21525915683947341 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1563 0.20819929587877284 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 1553 0.20686724664090483 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1482 0.1974096970520418 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1468 0.19554482811902657 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1460 0.19447918872873218 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 1459 0.19434598380494536 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1422 0.18941740162483367 No Hit CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 1335 0.17782857325538182 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1280 0.17050230244710765 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1215 0.16184398240096548 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1207 0.16077834301067104 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1184 0.1577146297635746 No Hit ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC 1168 0.15558335098298573 RNA PCR Primer, Index 15 (96% over 25bp) ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 1167 0.15545014605919893 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1164 0.15505053128783852 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1147 0.15278604758346287 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1101 0.14665862108926994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1083 0.1442609324611075 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1069 0.14239606352809225 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1055 0.140531194595077 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1045 0.13919914535720898 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1041 0.13866632566206177 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1003 0.13360453855816326 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 995 0.13253889916786885 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 965 0.12854275145426478 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 961 0.12800993175911754 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 949 0.12641147267367592 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 947 0.1261450628261023 No Hit GTACATGGGCTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAA 935 0.12454660374066066 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 918 0.12228212003628502 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 894 0.11908520186540177 RNA PCR Primer, Index 33 (96% over 29bp) GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 894 0.11908520186540177 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 892 0.11881879201782815 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 890 0.11855238217025454 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 878 0.11695392308481291 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 864 0.11508905415179767 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 854 0.11375700491392965 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 818 0.10896162765760473 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 813 0.10829560303867072 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 810 0.10789598826731032 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 808 0.10762957841973671 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 806 0.1073631685721631 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 772 0.1028342011634118 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 766 0.10203497162069097 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 761 0.10136894700175697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.664098475736057E-4 0.0 2 0.0 0.0 0.0 3.9961477136040856E-4 0.0 3 0.0 0.0 0.0 3.9961477136040856E-4 0.0 4 0.0 0.0 0.0 3.9961477136040856E-4 0.0 5 0.0 0.0 0.0 3.9961477136040856E-4 0.0 6 0.0 0.0 0.0 3.9961477136040856E-4 0.0 7 0.0 0.0 0.0 3.9961477136040856E-4 0.0 8 0.0 0.0 0.0 5.328196951472114E-4 0.0 9 0.0 0.0 0.0 5.328196951472114E-4 0.0 10-11 0.0 0.0 0.0 8.658320046142185E-4 0.0 12-13 0.0 0.0 0.0 0.0011988443140812256 0.0 14-15 0.0 0.0 0.0 0.0014652541616548315 0.0 16-17 0.0 0.0 0.0 0.0019314713949086415 0.0 18-19 6.660246189340143E-5 0.0 0.0 0.003396725556563473 0.0 20-21 1.3320492378680285E-4 0.0 0.0 0.017849459787431583 0.0 22-23 1.3320492378680285E-4 0.0 0.0 0.06646925696961463 0.0 24-25 1.3320492378680285E-4 0.0 0.0 0.15671559283517356 0.0 26-27 1.3320492378680285E-4 0.0 0.0 0.31689451368880395 0.0 28-29 1.3320492378680285E-4 0.0 0.0 0.7531406390905833 0.0 30-31 1.3320492378680285E-4 0.0 0.0 1.4807059328141006 0.0 32-33 1.3320492378680285E-4 0.0 0.0 2.4697524919311116 0.0 34-35 1.3320492378680285E-4 0.0 0.0 4.000476873627157 0.0 36-37 1.3320492378680285E-4 0.0 0.0 5.802006865381772 0.0 38-39 1.3320492378680285E-4 0.0 0.0 7.630177841893747 0.0 40-41 2.664098475736057E-4 0.0 0.0 9.459347855334125 0.0 42-43 2.664098475736057E-4 0.0 0.0 10.907285376896672 0.0 44-45 2.664098475736057E-4 0.0 0.0 12.25771689424728 0.0 46-47 2.664098475736057E-4 0.0 0.0 13.791238579342846 0.0 48-49 2.664098475736057E-4 0.0 0.0 15.216331456475956 0.0 50-51 3.9961477136040856E-4 0.0 0.0 16.34597581264994 0.0 52-53 3.9961477136040856E-4 0.0 0.0 17.228125420428043 0.0 54-55 3.9961477136040856E-4 0.0 0.0 18.16682051835364 0.0 56-57 6.660246189340143E-4 0.0 0.0 19.192565033973917 0.0 58-59 6.660246189340143E-4 0.0 0.0 19.973612104597834 0.0 60-61 6.660246189340143E-4 0.0 0.0 20.688922545332964 0.0 62-63 6.660246189340143E-4 0.0 0.0 21.297069624881615 0.0 64-65 6.660246189340143E-4 0.0 0.0 21.879774563986985 0.0 66-67 6.660246189340143E-4 0.0 0.0 22.50197476299514 0.0 68-69 6.660246189340143E-4 0.0 0.0 23.22454487207665 0.0 70-71 6.660246189340143E-4 0.0 0.0 24.011186549499616 0.0 72-73 6.660246189340143E-4 0.0 0.0 24.663424458821694 0.0 74-75 6.660246189340143E-4 0.0 0.0 25.29674726896605 0.0 76-77 6.660246189340143E-4 0.0 0.0 25.85833922765121 0.0 78-79 6.660246189340143E-4 0.0 0.0 26.525496088437414 0.0 80-81 6.660246189340143E-4 0.0 0.0 27.133976180295527 0.0 82-83 6.660246189340143E-4 0.0 0.0 27.715282467701137 0.0 84-85 6.660246189340143E-4 0.0 0.0 28.35193540094016 0.0 86-87 6.660246189340143E-4 0.0 0.0 28.93763745083073 0.0 88 6.660246189340143E-4 0.0 0.0 29.383807343054627 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 40 1.027729E-9 82.33034 1 CGAGAGT 20 0.002160031 70.521835 4 GTACCCA 520 0.0 63.331028 1 CGTCAGA 55 1.2816599E-8 59.8407 2 GTTATGG 40 7.984881E-6 58.807384 1 TACCCAT 615 0.0 53.51607 2 CTATACA 230 0.0 53.18233 1 ACCCATG 620 0.0 53.080956 3 CTTAATA 135 0.0 52.27323 1 CTTTACA 410 0.0 45.89845 1 GTCAGAT 90 1.3091267E-8 41.790718 3 TAATACA 175 0.0 40.29819 3 TATAACG 60 8.801621E-5 39.181408 2 ATTAACG 50 0.0016990642 37.61165 4 CTTAACA 140 1.2732926E-11 36.96464 1 CAGATGT 90 6.171467E-7 36.56688 5 TGGGATG 105 5.1031748E-8 35.813457 6 AGACTAG 360 0.0 35.248 14-15 TATAAGA 175 0.0 34.913467 9 GTACCGG 55 0.0027034383 34.215206 1 >>END_MODULE