FastQCFastQC Report
Mon 6 Jun 2016
ERR522865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10227396
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380980.3725092877991622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305320.2985315128112767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198140.19373455374173446No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141570.13842233154949707No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG209300.058.818982
GTACCTG238600.052.2415541
ACCTGGG234900.051.6490443
CCTGGGG202900.041.505654
TATAACG10700.031.6421362
GGTATCA265100.031.387651
GTATCAA395350.031.171681
CTGGGGG129700.030.5976165
TGGGGGG200900.028.3985356
TATCACG10700.028.1263432
CATGGGG269800.027.1879394
GTACATG432600.026.5521581
ATGGGGG178700.026.1545315
TCAACGC466150.026.0349034
TACATGG438650.025.964092
CAACGCA472400.025.7198095
ATAACGC13200.025.649813
ATCAACG472850.025.6480033
TATCAAC474800.025.6213952
GTACCCG19350.025.0378441
Produced by FastQC (version 0.11.5)