##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522865_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10227396
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330390257696095	33.0	31.0	34.0	28.0	34.0
2	31.664846261941946	34.0	31.0	34.0	27.0	35.0
3	31.645852864209033	34.0	31.0	34.0	27.0	36.0
4	33.948230126221766	35.0	33.0	37.0	31.0	37.0
5	34.04325666083527	35.0	34.0	37.0	31.0	37.0
6	34.26460606394824	35.0	35.0	37.0	30.0	37.0
7	34.1518440275511	35.0	34.0	37.0	30.0	37.0
8	34.25292029368961	35.0	34.0	37.0	30.0	37.0
9	35.4210584981749	37.0	35.0	39.0	30.0	39.0
10-11	35.3801075073264	37.0	34.5	39.0	30.0	39.0
12-13	35.40237197229872	37.0	35.0	39.0	30.0	39.0
14-15	36.16244472199962	38.0	35.0	40.0	30.0	41.0
16-17	36.316823070114815	38.0	36.0	40.0	30.0	41.0
18-19	36.17885847971468	38.0	35.5	40.0	29.5	41.0
20-21	36.27967847338658	38.0	35.0	40.0	30.0	41.0
22-23	36.12726083941601	38.0	35.0	40.0	29.5	41.0
24-25	36.10496757923522	38.0	35.0	40.0	29.5	41.0
26-27	35.98859318637902	37.5	36.0	40.0	29.5	41.0
28-29	35.75026331238176	38.0	35.0	40.0	29.0	41.0
30-31	35.55949060738432	37.0	35.0	40.0	28.0	41.0
32-33	35.52174121350146	37.5	34.5	40.0	27.0	41.0
34-35	35.366609496689094	37.5	34.5	40.0	27.0	41.0
36-37	35.18802293369691	37.5	34.5	40.0	27.0	41.0
38-39	35.021341502763754	36.5	34.5	40.0	26.0	41.0
40-41	34.971910885234124	37.0	34.0	40.0	25.5	41.0
42-43	34.766240546469504	36.0	34.0	40.0	25.0	41.0
44-45	34.6855543678958	37.0	33.5	40.0	25.0	41.0
46-47	34.4641954315644	36.5	33.5	40.0	24.0	41.0
48-49	34.327100759567735	37.0	33.0	40.0	23.0	41.0
50-51	33.292126705566105	36.5	31.5	38.5	22.0	40.0
52-53	33.509505205430585	36.5	32.5	38.5	22.5	39.5
54-55	34.362364134526516	37.0	33.0	39.5	23.0	40.5
56-57	34.50170576166211	37.0	34.0	40.0	24.5	41.0
58-59	34.43052229521571	37.0	33.0	40.0	23.0	41.0
60-61	34.177948961788516	36.5	33.5	39.5	23.0	41.0
62-63	34.00151812836816	36.5	33.0	39.0	22.5	41.0
64-65	33.60386192145097	36.0	33.0	39.0	22.0	41.0
66-67	33.409921743521025	36.0	33.0	38.5	22.0	40.5
68-69	33.07488978621733	35.0	32.5	37.5	21.0	40.0
70-71	32.86652389327645	35.0	32.0	37.5	20.5	40.0
72-73	32.54270740078901	35.0	32.0	37.5	20.5	39.0
74-75	32.166007701276065	35.0	31.5	36.5	20.0	39.0
76-77	32.044189791810155	35.0	31.0	37.0	19.0	38.5
78-79	31.765723454924398	35.0	31.0	36.5	19.0	38.0
80-81	31.50655303656962	35.0	30.0	36.0	18.0	37.5
82-83	31.191991734748512	34.5	30.0	35.5	18.0	37.0
84-85	30.95261965997992	35.0	30.0	35.5	17.5	37.0
86-87	30.70248922599653	34.0	29.5	35.0	17.5	37.0
88-89	30.545417719231757	34.0	29.0	35.0	17.5	36.5
90-91	30.33120791450727	34.0	29.0	35.0	17.0	36.0
92-93	30.071446485498363	34.0	29.0	35.0	16.5	36.0
94-95	29.964060353192544	34.0	29.0	35.0	16.0	36.0
96-97	29.769965297129396	34.0	29.0	35.0	15.5	36.0
98-99	29.673176437091122	34.0	27.5	35.0	15.0	36.0
100	28.431191869367336	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06601716865186447
1101	2	0.047251245499797534
1101	3	0.06570459661295303
1101	4	0.07938488870015448
1101	5	0.11387423979675759
1101	6	0.15813229545643281
1101	7	0.1340771199885893
1101	8	0.0739690007017586
1101	9	0.05844622165710689
1101	10-11	0.054005887844517986
1101	12-13	0.05381894203534898
1101	14-15	-0.047928070488623575
1101	16-17	0.0718030786397108
1101	18-19	0.05411927165521746
1101	20-21	0.029842640551088095
1101	22-23	0.07133569608501489
1101	24-25	0.02341165440351034
1101	26-27	0.04559451957177174
1101	28-29	-0.0772675885544416
1101	30-31	-0.07533349482818608
1101	32-33	-0.09508737273005607
1101	34-35	-0.1285830034199691
1101	36-37	-0.13313029944343668
1101	38-39	-0.17252807098683576
1101	40-41	-0.18639231727571826
1101	42-43	-0.1647733480954372
1101	44-45	-0.12548433528872494
1101	46-47	-0.1938418312484913
1101	48-49	-0.3211647445016865
1101	50-51	-0.3333307172815694
1101	52-53	-0.2467879636406778
1101	54-55	-0.2416962749304119
1101	56-57	-0.2658900596614444
1101	58-59	-0.19419917707282508
1101	60-61	-0.1765530736717409
1101	62-63	-0.2923789244933559
1101	64-65	-0.22663884400267165
1101	66-67	-0.20827696632527903
1101	68-69	-0.20576357072886964
1101	70-71	-0.3246922685216447
1101	72-73	-0.2266501660148279
1101	74-75	-0.21539139123921558
1101	76-77	-0.2513722506879219
1101	78-79	-0.3787896668633941
1101	80-81	-0.3698632899689045
1101	82-83	-0.5743743890377537
1101	84-85	-0.5036865155734489
1101	86-87	-0.3903404009739404
1101	88-89	-0.4385368869600015
1101	90-91	-0.48746661343255226
1101	92-93	-0.5108232155964174
1101	94-95	-0.5044115063547068
1101	96-97	-0.544068269203553
1101	98-99	-0.4401090592450636
1101	100	-0.37415347654792797
1102	1	0.04436887481806551
1102	2	-0.0021793816426729506
1102	3	-0.020596545215454398
1102	4	-0.011651676114425413
1102	5	-0.0011881949529666258
1102	6	0.03238854509678646
1102	7	0.02868128203122211
1102	8	0.12546369436456928
1102	9	0.052256899273487534
1102	10-11	0.04562672518535038
1102	12-13	-0.004586526245823563
1102	14-15	-0.04852097454708115
1102	16-17	-0.021266314769086136
1102	18-19	-0.034518857521824486
1102	20-21	-0.10647545607275077
1102	22-23	-0.03339171190695822
1102	24-25	-0.11111370380508134
1102	26-27	-0.08517098430419168
1102	28-29	-0.03849859918356202
1102	30-31	-0.17196565591724067
1102	32-33	-0.10098024308444309
1102	34-35	-0.2620793083021269
1102	36-37	-0.6796709560153218
1102	38-39	-0.2599295790388183
1102	40-41	-0.8679477604843555
1102	42-43	-0.8707035703779411
1102	44-45	-0.3518007467086477
1102	46-47	-0.33597521719307366
1102	48-49	-0.4155691996053861
1102	50-51	-0.3923381835569444
1102	52-53	-0.26838832164375503
1102	54-55	-0.0872823765194326
1102	56-57	0.032467020661130164
1102	58-59	-0.035585627211339954
1102	60-61	-0.08120320889908328
1102	62-63	-0.29691720944899913
1102	64-65	-0.07206176853020452
1102	66-67	-0.09568828760734505
1102	68-69	-0.11595725614650831
1102	70-71	-0.24365178869065574
1102	72-73	-0.14578123788671604
1102	74-75	-0.10331207567862322
1102	76-77	-0.2519226650014694
1102	78-79	-0.2663083685165404
1102	80-81	-0.27844513950074656
1102	82-83	-0.3534811487605616
1102	84-85	-0.5075744043006658
1102	86-87	-0.5410395037056155
1102	88-89	-0.462757377395441
1102	90-91	-0.444363207676556
1102	92-93	-0.41258934012065396
1102	94-95	-0.6591637813799842
1102	96-97	-0.6980294745764759
1102	98-99	-0.6916656122221205
1102	100	-0.6427673757809522
1103	1	-0.013766012645476167
1103	2	-0.06609995764310739
1103	3	-0.03402469003317776
1103	4	-0.04221749129557395
1103	5	0.0024197042629978682
1103	6	0.05884336754580488
1103	7	0.05432562319052181
1103	8	-0.009922996698996656
1103	9	-0.023523934148784065
1103	10-11	-0.0343991975486162
1103	12-13	0.035034684333105304
1103	14-15	-0.018214458678919243
1103	16-17	-0.12487835759198163
1103	18-19	-0.09710141863283894
1103	20-21	-0.13200581205484951
1103	22-23	-0.16064208632245425
1103	24-25	-0.19834198652368684
1103	26-27	-0.1699624765640806
1103	28-29	-0.2672687108429699
1103	30-31	-0.319903044474259
1103	32-33	-0.37401215831713586
1103	34-35	-0.38479477740470003
1103	36-37	-0.5271882157318757
1103	38-39	-0.3994359304787878
1103	40-41	-0.2526674850609538
1103	42-43	-0.34907088848542145
1103	44-45	-0.21844423796584778
1103	46-47	-0.23033319768747873
1103	48-49	-0.3784639912662726
1103	50-51	-0.4283368123623532
1103	52-53	-0.2407807127887338
1103	54-55	-0.19062228821433536
1103	56-57	-0.05844956575528215
1103	58-59	-0.11630844705204169
1103	60-61	-0.07644134085825272
1103	62-63	-0.15019405195060642
1103	64-65	-0.324787315954147
1103	66-67	-0.15420336899991582
1103	68-69	-0.2022352134120382
1103	70-71	-0.2018794600473086
1103	72-73	-0.17149613346473558
1103	74-75	-0.09532791063186608
1103	76-77	-0.1467624040632174
1103	78-79	-0.23492275664151663
1103	80-81	-0.15278326769674777
1103	82-83	-0.22373945895447278
1103	84-85	-0.25799461539636326
1103	86-87	-0.868035164536888
1103	88-89	-0.30109486153303777
1103	90-91	-0.6646066502386603
1103	92-93	-0.37392103575246693
1103	94-95	-0.6877346708859235
1103	96-97	-0.46878444181525225
1103	98-99	-0.4124695110375036
1103	100	-0.4113396697948488
1104	1	0.05521079002746987
1104	2	-0.036570292838000285
1104	3	-0.049777675552629574
1104	4	0.017676641383850722
1104	5	-0.06466192310059427
1104	6	0.03540190591610326
1104	7	-0.07677314052011042
1104	8	0.03471284180160694
1104	9	-0.07075028184735288
1104	10-11	-0.10128995790898188
1104	12-13	0.043900437833819694
1104	14-15	-0.061127347255045095
1104	16-17	-0.05300795810611447
1104	18-19	0.026453850864406547
1104	20-21	-0.08396680868809625
1104	22-23	-0.07048988373269083
1104	24-25	-0.055539314674234674
1104	26-27	-0.06042272668917548
1104	28-29	-0.05319838516754061
1104	30-31	-0.04008831889036912
1104	32-33	-0.04003359330248202
1104	34-35	-0.08529730747216746
1104	36-37	-0.0886922952739937
1104	38-39	-0.06461551526364673
1104	40-41	-0.06804599303602288
1104	42-43	-0.21530803986810554
1104	44-45	-0.1999571535029645
1104	46-47	-0.11662141399585124
1104	48-49	-0.0761397223830187
1104	50-51	-0.2181043787673076
1104	52-53	-0.15118948115565445
1104	54-55	-0.02546738109105462
1104	56-57	0.02951216792558853
1104	58-59	0.0304813412797742
1104	60-61	-0.07467099681659306
1104	62-63	-0.0031994852634156246
1104	64-65	-0.10244950621392235
1104	66-67	-0.01791653976207641
1104	68-69	-0.10560107776661454
1104	70-71	-0.07878562776367204
1104	72-73	-0.035929574900151806
1104	74-75	-0.015058504341347145
1104	76-77	-0.07856737568578609
1104	78-79	-0.10464759273804702
1104	80-81	-0.1573411357613992
1104	82-83	-0.10854266636557597
1104	84-85	-0.09705286644491196
1104	86-87	-0.1438112894414445
1104	88-89	-0.1816502848172732
1104	90-91	-0.28528838209692964
1104	92-93	-0.24033652477592327
1104	94-95	-0.2699406359639802
1104	96-97	-0.2806355633578086
1104	98-99	-0.2500308132042868
1104	100	-0.3098640279424387
1105	1	0.06016673349553159
1105	2	0.1270342344089812
1105	3	0.11647432787854584
1105	4	0.11960640655410515
1105	5	0.14884672168017232
1105	6	0.19885254186594636
1105	7	0.07360367109110655
1105	8	-0.009866642720758989
1105	9	0.09010623264047979
1105	10-11	0.13271128282798372
1105	12-13	0.15768819218160246
1105	14-15	0.14140343626257135
1105	16-17	0.1668669791685815
1105	18-19	0.12746893081260424
1105	20-21	0.07167868391152155
1105	22-23	0.12425733212957368
1105	24-25	0.0974773964375828
1105	26-27	0.12530266935150536
1105	28-29	0.016730432648238036
1105	30-31	-0.0081225842829582
1105	32-33	-0.01746658550896285
1105	34-35	0.01734568824580407
1105	36-37	0.03731481056933461
1105	38-39	0.03505813951183967
1105	40-41	0.030017374354230242
1105	42-43	0.07887577204113683
1105	44-45	0.04301592735707516
1105	46-47	0.0443134401720684
1105	48-49	-0.028477564601246286
1105	50-51	-0.07972120293752738
1105	52-53	0.06232054787395214
1105	54-55	0.11453484740284381
1105	56-57	0.23368276783995867
1105	58-59	0.15520464681122093
1105	60-61	0.12570597865446587
1105	62-63	0.17341869796525344
1105	64-65	0.1506743790194207
1105	66-67	0.20085325823688294
1105	68-69	0.15939877720519036
1105	70-71	0.1508268300063449
1105	72-73	0.08002655879207055
1105	74-75	0.14138621343109747
1105	76-77	0.13611409471517533
1105	78-79	0.16595338794600778
1105	80-81	0.2438607737585201
1105	82-83	0.1268254247224938
1105	84-85	0.14113637758940456
1105	86-87	0.09853811639052878
1105	88-89	0.03705734915857306
1105	90-91	0.03511734368455066
1105	92-93	0.039152086744714154
1105	94-95	0.08543186124196822
1105	96-97	0.08184241033275441
1105	98-99	0.06280750296020798
1105	100	0.11851064722987559
1106	1	0.03810367730081765
1106	2	0.01957562428391313
1106	3	0.05238259573436821
1106	4	0.10990914206328739
1106	5	0.09068361262815472
1106	6	0.12725183375439997
1106	7	0.14082892331320096
1106	8	-1.0438574917761656
1106	9	0.12128126122519944
1106	10-11	0.08748652717323324
1106	12-13	0.07545772322347233
1106	14-15	0.04387929688027015
1106	16-17	0.03046484624798751
1106	18-19	0.11628001424933387
1106	20-21	0.06836471018838353
1106	22-23	0.03427514703395218
1106	24-25	0.14449157208404984
1106	26-27	0.05770134546579442
1106	28-29	-0.06131043382823265
1106	30-31	0.037633968930244066
1106	32-33	0.05638783593354191
1106	34-35	0.09419753850056622
1106	36-37	0.08892736078664853
1106	38-39	0.07161299420025102
1106	40-41	0.08448995889237665
1106	42-43	0.08926666406467376
1106	44-45	-0.0311543825921774
1106	46-47	0.0689794406603923
1106	48-49	0.00834742674571487
1106	50-51	-0.00975304380907005
1106	52-53	0.03255628991344395
1106	54-55	0.028984420423917356
1106	56-57	-0.030143048755540747
1106	58-59	0.08006803329700318
1106	60-61	0.1324305229183409
1106	62-63	0.08349373022966944
1106	64-65	0.07468945657476667
1106	66-67	0.05513878196689603
1106	68-69	0.06917447480468297
1106	70-71	0.0037031306927204355
1106	72-73	0.1069822358925947
1106	74-75	0.09109182335800625
1106	76-77	0.07961680044181918
1106	78-79	0.08016384778921193
1106	80-81	0.11854350536569314
1106	82-83	0.07858653309299868
1106	84-85	0.10368690154365368
1106	86-87	0.05955053210727712
1106	88-89	0.013165766617042607
1106	90-91	0.01672886186205602
1106	92-93	0.14730603009502374
1106	94-95	0.07353485229838697
1106	96-97	0.08097617725696082
1106	98-99	0.11182660997458882
1106	100	0.10925609619306442
1107	1	-0.0646807744367699
1107	2	-0.06234913461723224
1107	3	-0.11387465191939228
1107	4	0.052155962600622274
1107	5	0.03416718779154593
1107	6	0.030152815867865
1107	7	0.08482417050878155
1107	8	0.12909205770174736
1107	9	0.10056572938120212
1107	10-11	0.07786647487423437
1107	12-13	0.12627574673695818
1107	14-15	0.07126805904018596
1107	16-17	0.08467058098899116
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2310	4	0.15777209506455137
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2314	1	-0.33041388243550784
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2314	82-83	0.37120109977498217
2314	84-85	0.44815227140946234
2314	86-87	0.4838380520709187
2314	88-89	0.49963082116335755
2314	90-91	0.4339885143418627
2314	92-93	0.5065958475404955
2314	94-95	0.4862680161429971
2314	96-97	0.639981065607973
2314	98-99	0.6464124753917666
2314	100	0.6309502211832658
2315	1	0.11576739356959465
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2315	4	0.09068600960902273
2315	5	0.04264583602925853
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2315	10-11	-1.293377706904046
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2315	16-17	0.05086475866626472
2315	18-19	0.14594771762764935
2315	20-21	0.11142148416605124
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2315	24-25	0.10032757610059662
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2315	96-97	0.6268261467538103
2315	98-99	0.6875617543187893
2315	100	0.6648171812632135
2316	1	-0.0741170650266163
2316	2	-0.24532397488798097
2316	3	-0.1355505689846659
2316	4	-0.06014876479520126
2316	5	-0.1137375239466607
2316	6	-0.04281481368830242
2316	7	-0.02594195596365978
2316	8	0.0027868444281935467
2316	9	-0.017769835750954144
2316	10-11	-0.17212424209969868
2316	12-13	-0.0021105780974934873
2316	14-15	-0.04400061327823579
2316	16-17	-0.12577185430978233
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2316	30-31	0.05722428026683701
2316	32-33	-0.020379841183704173
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2316	36-37	0.10144602481790344
2316	38-39	-0.017192180005544344
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2316	52-53	0.1465854369310975
2316	54-55	0.0767087023951305
2316	56-57	-0.05242425320911792
2316	58-59	-0.07469701465250722
2316	60-61	-0.016164888269628364
2316	62-63	-0.0020007413907379146
2316	64-65	-0.25701899771582504
2316	66-67	-0.2595924939624652
2316	68-69	0.0350780236912982
2316	70-71	0.05692590122614405
2316	72-73	0.0029641857786728565
2316	74-75	-0.012807514536973486
2316	76-77	-0.08389391634855414
2316	78-79	-0.17649687669997505
2316	80-81	0.04289707660335296
2316	82-83	-0.009993520045490811
2316	84-85	0.12505266956112848
2316	86-87	0.16412750930551567
2316	88-89	0.11834206393448454
2316	90-91	0.3474560325031817
2316	92-93	0.3629630900698757
2316	94-95	0.3548532928475403
2316	96-97	0.3784936046508385
2316	98-99	0.46239275935484514
2316	100	0.12890801755627024
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	18252.0
1	1660.0
2	1045.0
3	821.0
4	721.0
5	622.0
6	763.0
7	2628.0
8	5403.0
9	6745.0
10	9434.0
11	14403.0
12	20779.0
13	26778.0
14	31771.0
15	37147.0
16	43615.0
17	53040.0
18	64685.0
19	76973.0
20	89494.0
21	104318.0
22	119597.0
23	129220.0
24	135750.0
25	142884.0
26	154103.0
27	172689.0
28	200236.0
29	235060.0
30	283618.0
31	350042.0
32	443467.0
33	589148.0
34	849366.0
35	1344923.0
36	1462304.0
37	1414416.0
38	1304454.0
39	284689.0
40	333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.182249388767424	17.004108959545015	11.922729274273124	23.89091237741444
2	16.65890257072299	19.786341081480348	39.999653624453174	23.55510272334349
3	19.35269300951801	26.152010580687303	29.17959951686628	25.315696892928408
4	13.037797461861475	15.778739914604225	36.310033635558064	34.873428987976226
5	13.415561229518833	36.31285387417343	33.54240448431628	16.72918041199146
6	30.976348591607632	34.561982762989786	19.60445475850942	14.857213886893161
7	28.491486209921636	31.74342787283619	21.707363482179698	18.05772243506248
8	27.233979981501932	33.8780411653097	20.47551897767537	18.412459875512997
9	28.441652012181628	15.464724382445441	19.808991201818753	36.28463240355417
10-11	25.686326690142053	25.17036318236062	28.303709748166444	20.83960037933088
12-13	25.919437287818255	23.18575829617919	28.09947156283643	22.795332853166126
14-15	23.493293341901992	24.538599514252095	26.25569117154526	25.712415972300658
16-17	23.01516302906178	26.94861994280449	26.927420448358035	23.108796579775692
18-19	22.733138928568945	26.296712746718963	28.607612878549432	22.36253544616266
20-21	23.55395197636925	25.790483250043483	28.501737983174934	22.153826790412328
22-23	23.23911697067752	25.469615691059115	28.428243796636778	22.863023541626585
24-25	22.851554880863727	25.471785494522038	28.52709987867701	23.14955974593723
26-27	22.663009343551963	25.580874730851445	28.607605122800745	23.14851080279585
28-29	22.8112573019519	26.118232499392857	28.418578013408364	22.651932185246878
30-31	23.748041138132926	25.65138007080473	28.45770421634205	22.142874574720295
32-33	23.27995388616448	25.71229109079306	28.447945077445592	22.559809945596868
34-35	23.308393959293834	26.001050537992764	28.30069378728649	22.38986171542691
36-37	23.23845991970405	26.056174232470507	28.158422238769354	22.54694360905609
38-39	23.187111844618858	26.31204629645784	27.896885661071458	22.603956197851844
40-41	23.199316087123112	25.92586038948546	28.165654474497842	22.709169048893585
42-43	22.96378363524542	26.07127179370512	27.83811630422301	23.12682826682645
44-45	22.77875420938613	26.25777836929382	28.000149752000205	22.96331766931985
46-47	22.726573531606128	25.92210888995772	27.805296971832348	23.546020606603808
48-49	22.86801461883824	26.04427396898282	27.87855854879218	23.20915286338675
50-51	22.842967182713032	26.406980736973075	27.825108939863373	22.924943140450523
52-53	22.832403245282645	26.51305522296406	27.46312081900878	23.191420712744513
54-55	22.569028358368225	26.27758532737262	27.883522155635376	23.269864158623772
56-57	22.897063209699	26.36410847235977	27.34825709334445	23.39057122459678
58-59	22.767887011215798	26.45105571628307	27.69014350291209	23.090913769589047
60-61	23.335187701345212	26.361522177388725	27.66974842635998	22.633541694906086
62-63	23.008668622961274	26.39825196201343	27.723990895896915	22.86908851912838
64-65	22.826353666287055	26.144133639753836	27.611911108514363	23.417601585444743
66-67	22.798218828493763	26.248221490192446	27.434809705210256	23.518749976103535
68-69	22.5290010884094	26.24142178435513	27.678521225993215	23.55105590124225
70-71	22.560586743292994	26.059802523379382	27.82424707264035	23.55536366068727
72-73	22.503954916841078	26.53446409319943	27.245809739740924	23.715771250218573
74-75	22.621440467443527	26.47265090718819	27.28659522324871	23.619313402119573
76-77	22.4161173518762	26.114572210995707	27.43882530466013	24.03048513246796
78-79	22.359313189971346	26.206056244575393	27.508496150168547	23.92613441528471
80-81	22.501038871895403	26.326386248208383	27.140750060116837	24.031824819779377
82-83	22.6627555114592	26.40144962418694	27.206081245033648	23.72971361932022
84-85	22.660698664886837	26.5328156018741	26.88188379653591	23.92460193670316
86-87	22.395052832977765	26.499076641704384	27.12813485546064	23.977735669857214
88-89	22.20245628717297	26.17503662908034	27.365385525207202	24.257121558539485
90-91	22.89675195676433	25.944092615758052	27.263469390626483	23.89568603685113
92-93	22.704221334365236	26.033804298359485	27.22236831044922	24.03960605682606
94-95	22.52830779371945	25.868105592356493	27.139478760385543	24.464107853538508
96-97	22.409695940980683	25.81356330870633	27.322955628935773	24.453785121377216
98-99	22.405372994887333	25.82407431688739	27.063760934666615	24.70679175355866
100	22.414847190260513	25.899452276881448	27.12380107430567	24.561899458552375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9510.0
1	10134.5
2	12239.5
3	10628.5
4	5683.0
5	4139.5
6	5387.5
7	8724.0
8	13484.0
9	15897.0
10	13867.0
11	11451.0
12	11607.5
13	12428.5
14	13222.5
15	13914.5
16	14601.0
17	15602.5
18	16603.5
19	17608.5
20	18980.0
21	20676.0
22	22997.5
23	26539.5
24	31006.5
25	36468.5
26	43937.5
27	53309.5
28	63551.5
29	75353.0
30	89813.0
31	104668.0
32	120300.5
33	137927.5
34	156008.0
35	173509.5
36	194263.0
37	214642.5
38	231082.5
39	247424.5
40	260571.5
41	272492.5
42	286119.5
43	301477.0
44	317544.5
45	334305.0
46	352998.5
47	372268.5
48	392840.0
49	413980.5
50	437167.5
51	439828.5
52	422572.0
53	408811.0
54	394383.0
55	372231.0
56	342186.5
57	315234.5
58	288408.0
59	249491.5
60	203694.5
61	164417.0
62	134212.5
63	106703.5
64	80697.0
65	61029.0
66	46338.5
67	36181.5
68	28402.0
69	21765.0
70	17285.0
71	13548.5
72	10777.0
73	8593.0
74	6745.5
75	5116.0
76	3634.0
77	2457.0
78	1732.0
79	1221.0
80	874.5
81	649.0
82	465.0
83	335.0
84	253.5
85	199.5
86	164.0
87	134.0
88	105.0
89	73.0
90	51.5
91	43.5
92	36.5
93	29.0
94	17.0
95	15.5
96	19.5
97	15.0
98	10.5
99	5.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14505158497822906
2	0.07113247594988989
3	0.30761495888102897
4	0.3064416396901029
5	0.3080451759177018
6	0.2585115507407751
7	0.2588635464980529
8	0.25745556346894166
9	0.26836743194455365
10-11	0.246602360952876
12-13	0.25508448093727865
14-15	0.23020033642972268
16-17	0.2378171335108174
18-19	0.2508654206799072
20-21	0.2519947404011735
22-23	0.24194819482886942
24-25	0.24324862359881247
26-27	0.24299440444077847
28-29	0.25352005534937727
30-31	0.23862379045457904
32-33	0.2511440839877521
34-35	0.23517716533123387
36-37	0.24335617785798064
38-39	0.24418727895155326
40-41	0.2608826332724381
42-43	0.2471939093783012
44-45	0.23329007696582788
46-47	0.23577360258662128
48-49	0.24522859973350009
50-51	0.23861890162461688
52-53	0.25365694258831867
54-55	0.2633368259134583
56-57	0.2595822045024951
58-59	0.2579688906149718
60-61	0.23915178409049578
62-63	0.26556124354625554
64-65	0.2600759763286764
66-67	0.26539502332754106
68-69	0.2703474080792413
70-71	0.29022539070551295
72-73	0.29914750538651286
74-75	0.27966551798717876
76-77	0.2769619950180867
78-79	0.2730264868985224
80-81	0.25839421882168245
82-83	0.2689932021797142
84-85	0.2803499541818856
86-87	0.2732122624370857
88-89	0.28231526382668665
90-91	0.2759695625357618
92-93	0.25596447033047315
94-95	0.2767615529896369
96-97	0.27532912581071467
98-99	0.2615866247869937
100	0.2835032495074993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0227396E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.02960956032814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.24006934140263	38.49073131566688
2	16.57134066896114	17.906861324703147
3	5.832494632403425	9.453822232544
4	2.3640427266648953	5.109132220225514
5	1.193610859948487	3.224516436499213
6	0.6824922541361145	2.212487401135351
7	0.4343754416145323	1.6428394859119806
8	0.3108244566855171	1.3434979229215693
9	0.21737363684123412	1.0570151454516397
>10	1.0538751788644198	10.048495685913124
>50	0.06389814367993307	2.375082912549352
>100	0.031803132539810056	3.1563504048347895
>500	0.002374810145449219	0.8782427219526793
>1k	0.00114977090317758	1.0876829600261375
>5k	1.7496513315599094E-4	0.6276449571395615
>10k+	9.998007608913768E-5	1.3855968725250916
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38098	0.3725092877991622	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30532	0.2985315128112767	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19814	0.19373455374173446	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14157	0.13842233154949707	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9555319848766977E-5
2	0.0	0.0	0.0	0.0	1.9555319848766977E-5
3	0.0	0.0	0.0	0.0	1.9555319848766977E-5
4	0.0	0.0	0.0	0.0	2.933297977315047E-5
5	0.0	2.933297977315047E-5	0.0	0.0	2.933297977315047E-5
6	9.777659924383489E-6	2.933297977315047E-5	0.0	0.0	5.866595954630094E-5
7	9.777659924383489E-6	2.933297977315047E-5	0.0	0.0	5.866595954630094E-5
8	9.777659924383489E-6	2.933297977315047E-5	0.0	1.9555319848766977E-5	6.844361947068442E-5
9	9.777659924383489E-6	2.933297977315047E-5	0.0	1.9555319848766977E-5	6.844361947068442E-5
10-11	9.777659924383489E-6	2.933297977315047E-5	0.0	1.9555319848766977E-5	6.844361947068442E-5
12-13	9.777659924383489E-6	2.933297977315047E-5	0.0	2.933297977315047E-5	6.844361947068442E-5
14-15	9.777659924383489E-6	2.933297977315047E-5	0.0	2.933297977315047E-5	7.333244943287616E-5
16-17	9.777659924383489E-6	2.933297977315047E-5	0.0	4.888829962191744E-5	9.777659924383489E-5
18-19	9.777659924383489E-6	2.933297977315047E-5	0.0	5.866595954630094E-5	1.5155372882794409E-4
20-21	9.777659924383489E-6	2.933297977315047E-5	0.0	9.777659924383489E-5	1.5644255879013582E-4
22-23	9.777659924383489E-6	3.422180973534221E-5	0.0	2.591079879961625E-4	2.0044202844986153E-4
24-25	9.777659924383489E-6	3.9110639697533954E-5	0.0	6.991026845934196E-4	2.4444149810958724E-4
26-27	9.777659924383489E-6	3.9110639697533954E-5	0.0	0.0016328692073720426	2.4933032807177897E-4
28-29	9.777659924383489E-6	3.9110639697533954E-5	0.0	0.004835052832607635	2.6888564792054595E-4
30-31	9.777659924383489E-6	3.9110639697533954E-5	0.0	0.015052707453588382	2.737744778827377E-4
32-33	9.777659924383489E-6	3.9110639697533954E-5	0.0	0.040934173273431476	2.737744778827377E-4
34-35	2.933297977315047E-5	4.888829962191744E-5	0.0	0.08145768483003885	2.737744778827377E-4
36-37	2.933297977315047E-5	5.866595954630094E-5	0.0	0.14340404928097045	2.737744778827377E-4
38-39	2.933297977315047E-5	5.866595954630094E-5	0.0	0.2393228931391725	2.835521378071212E-4
40-41	3.9110639697533954E-5	5.866595954630094E-5	0.0	0.357182805867691	2.9332979773150466E-4
42-43	3.9110639697533954E-5	5.866595954630094E-5	0.0	0.465797941137705	2.9332979773150466E-4
44-45	3.9110639697533954E-5	5.866595954630094E-5	0.0	0.5952150478968449	3.0310745765588817E-4
46-47	3.9110639697533954E-5	5.866595954630094E-5	0.0	0.745087019217795	3.2266277750465515E-4
48-49	3.9110639697533954E-5	5.866595954630094E-5	0.0	0.9056459728360964	3.324404374290386E-4
50-51	3.9110639697533954E-5	5.866595954630094E-5	0.0	1.075474148062713	3.324404374290386E-4
52-53	4.888829962191744E-5	5.866595954630094E-5	0.0	1.2509049224260016	3.7643990708876435E-4
54-55	4.888829962191744E-5	5.866595954630094E-5	0.0	1.4597655160707572	4.2043937674849003E-4
56-57	4.888829962191744E-5	5.866595954630094E-5	0.0	1.6865290050370594	4.302170366728735E-4
58-59	4.888829962191744E-5	6.844361947068442E-5	0.0	1.9384015246891781	4.448835265594488E-4
60-61	5.377712958410919E-5	6.844361947068442E-5	0.0	2.1945517705582143	4.7910533629479097E-4
62-63	5.866595954630094E-5	6.844361947068442E-5	0.0	2.461672550862409	4.7910533629479097E-4
64-65	5.866595954630094E-5	6.844361947068442E-5	0.0	2.7328070605655634	4.8399416625698275E-4
66-67	5.866595954630094E-5	6.844361947068442E-5	0.0	3.037738051797349	4.888829962191745E-4
68-69	5.866595954630094E-5	6.844361947068442E-5	0.0	3.4062824984971733	4.937718261813662E-4
70-71	5.866595954630094E-5	6.844361947068442E-5	0.0	3.801368403061737	5.475489557654754E-4
72-73	8.79989393194514E-5	6.844361947068442E-5	0.0	4.155309914664495	6.159925752361598E-4
74-75	8.79989393194514E-5	6.844361947068442E-5	0.0	4.513646484403264	6.257702351605433E-4
76-77	9.777659924383489E-5	8.79989393194514E-5	0.0	4.915038979619055	6.257702351605433E-4
78-79	1.0755425916821839E-4	8.79989393194514E-5	0.0	5.378514726524719	6.257702351605433E-4
80-81	1.1244308913041013E-4	8.79989393194514E-5	0.0	5.827778644730291	6.795473647446524E-4
82-83	1.2222074905479362E-4	8.79989393194514E-5	0.0	6.293361477349659	7.431021542531451E-4
84-85	1.2710957901698535E-4	8.79989393194514E-5	0.0	6.768800191172807	7.431021542531451E-4
86-87	1.2710957901698535E-4	8.79989393194514E-5	0.0	7.264762213177235	7.431021542531451E-4
88	1.3688723894136884E-4	8.79989393194514E-5	0.0	7.65498862075938	7.724351340262956E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	20930	0.0	58.81898	2
GTACCTG	23860	0.0	52.241554	1
ACCTGGG	23490	0.0	51.649044	3
CCTGGGG	20290	0.0	41.50565	4
TATAACG	1070	0.0	31.642136	2
GGTATCA	26510	0.0	31.38765	1
GTATCAA	39535	0.0	31.17168	1
CTGGGGG	12970	0.0	30.597616	5
TGGGGGG	20090	0.0	28.398535	6
TATCACG	1070	0.0	28.126343	2
CATGGGG	26980	0.0	27.187939	4
GTACATG	43260	0.0	26.552158	1
ATGGGGG	17870	0.0	26.154531	5
TCAACGC	46615	0.0	26.034903	4
TACATGG	43865	0.0	25.96409	2
CAACGCA	47240	0.0	25.719809	5
ATAACGC	1320	0.0	25.64981	3
ATCAACG	47285	0.0	25.648003	3
TATCAAC	47480	0.0	25.621395	2
GTACCCG	1935	0.0	25.037844	1
>>END_MODULE