FastQCFastQC Report
Mon 6 Jun 2016
ERR522865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10227396
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333460.32604584783849183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295640.28906673800447347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188970.1847684395910748No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122690.11996210961226102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG211850.060.4743042
GTACCTG242950.053.496071
ACCTGGG239650.052.669813
CCTGGGG209850.041.2490354
CTGGGGG122600.035.033765
GTACCCG20950.031.8716891
GGTATCA273350.031.2906361
TATAACG11100.030.0613782
TGGGGGG183600.029.3576836
GTATCAA397150.028.8300361
ATAACGC11900.028.432743
TAACGCA12650.027.8614644
TACCCGG22850.027.355142
TATCACG10350.025.4285262
ATGGGGG170800.024.9821055
CATGGGG262900.024.7388044
GTACATG431750.024.428551
TCAACGC467750.024.12194
CAACGCA474750.023.8057125
ATCAACG476000.023.7136943
Produced by FastQC (version 0.11.5)