FastQCFastQC Report
Mon 6 Jun 2016
ERR522864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6797207
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT287590.4231002527950083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172810.254236776958536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132740.1952860932438868No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79270.11662142994909527No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT73220.10772071528791165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70980.10442524407451473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG117600.050.4593352
GTACCTG132500.045.3635561
ACCTGGG132950.043.6434943
CCTGGGG124600.032.3017464
TATAACG6450.026.9731392
TATCACG6700.025.2648752
CTGGGGG75650.022.062325
CATGGGG176050.020.9120184
GTACATG334000.020.319451
GTACCCG11650.020.1853451
GAGTACT158950.019.7627212-13
TACATGG346350.019.3594862
ATGGGGG104000.019.1674485
ACATGGG344450.018.8930723
GTACACG15200.018.8746241
TAACGCA8750.017.7327524
TGGGGGG99500.017.7101426
AGTACTT176450.017.2168112-13
GTACTTT182350.017.18808414-15
GTATAGG19400.016.4853921
Produced by FastQC (version 0.11.5)