##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522864_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6797207
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.147778050602255	33.0	31.0	34.0	27.0	34.0
2	31.492597327108033	34.0	31.0	34.0	27.0	35.0
3	31.453712237982455	33.0	31.0	34.0	26.0	36.0
4	33.739533311255634	35.0	33.0	37.0	30.0	37.0
5	33.828264903511105	35.0	34.0	37.0	30.0	37.0
6	34.05330218720719	35.0	35.0	37.0	30.0	37.0
7	33.929547827512096	35.0	34.0	37.0	30.0	37.0
8	34.01568511890251	35.0	34.0	37.0	30.0	37.0
9	35.16380036682714	37.0	34.0	39.0	29.0	39.0
10-11	35.12148710492413	37.0	34.5	39.0	30.0	39.0
12-13	35.154269393296396	37.0	35.0	39.0	29.5	39.0
14-15	35.890760572688166	38.0	35.0	40.0	30.0	41.0
16-17	36.0557085432296	38.0	36.0	40.0	29.0	41.0
18-19	35.91063219054533	38.0	35.0	40.0	28.5	41.0
20-21	35.99603197313249	38.0	35.0	40.0	28.5	41.0
22-23	35.84814100850541	38.0	35.0	40.0	28.5	41.0
24-25	35.816631522329686	37.5	35.0	40.0	27.5	41.0
26-27	35.697152889414724	37.0	35.5	40.0	27.0	41.0
28-29	35.46866779252125	37.5	34.5	40.0	27.0	41.0
30-31	35.298819500421274	37.0	34.5	40.0	27.0	41.0
32-33	35.273677482530694	37.5	34.0	40.0	26.0	41.0
34-35	35.116849391227895	37.5	34.0	40.0	26.0	41.0
36-37	34.94538418500422	37.5	34.0	40.0	26.0	41.0
38-39	34.783602882772286	36.5	34.0	40.0	25.0	41.0
40-41	34.74189987152076	37.0	33.0	40.0	24.5	41.0
42-43	34.541190079984325	36.0	34.0	40.0	24.0	41.0
44-45	34.45050202237478	37.0	33.0	40.0	24.0	41.0
46-47	34.25253607842162	36.5	33.0	40.0	23.0	41.0
48-49	34.114287088799855	37.0	33.0	40.0	22.5	41.0
50-51	33.10059801032983	36.0	31.5	38.5	21.0	40.0
52-53	33.33653734541261	36.5	32.0	38.5	22.0	39.5
54-55	34.19632232768548	37.0	33.0	39.5	23.0	40.5
56-57	34.33009918632756	37.0	33.0	40.0	23.5	41.0
58-59	34.25481804511765	37.0	33.0	40.0	23.0	41.0
60-61	33.99400569969401	36.5	33.0	39.0	22.5	41.0
62-63	33.81973794530606	36.0	32.5	39.0	22.5	41.0
64-65	33.43030821335881	36.0	33.0	39.0	22.0	41.0
66-67	33.22453207618953	36.0	32.5	38.0	21.5	40.5
68-69	32.879245990301605	35.0	31.5	37.5	20.0	40.0
70-71	32.655004180393504	35.0	31.0	37.0	20.0	39.5
72-73	32.32458662800765	35.0	31.0	37.0	20.0	39.0
74-75	31.95870083109136	35.0	31.0	36.5	20.0	39.0
76-77	31.832078970082858	35.0	31.0	37.0	18.5	38.5
78-79	31.558014711042347	35.0	30.5	36.0	18.0	38.0
80-81	31.277300514755545	35.0	30.0	36.0	18.0	37.5
82-83	30.96541277910177	34.0	29.5	35.5	18.0	37.0
84-85	30.729097701452964	34.0	29.0	35.0	17.5	37.0
86-87	30.503440162996363	34.0	29.0	35.0	17.0	37.0
88-89	30.338931784775717	34.0	29.0	35.0	17.5	36.5
90-91	30.12003724470948	34.0	29.0	35.0	17.0	36.0
92-93	29.858329605086325	34.0	28.5	35.0	16.5	36.0
94-95	29.760905251230398	34.0	28.5	35.0	16.0	36.0
96-97	29.570434665297086	34.0	27.0	35.0	15.0	36.0
98-99	29.49166598869212	34.0	27.0	35.0	15.0	36.0
100	28.22686435766926	32.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13574508133384455
1101	2	0.14006160741312712
1101	3	0.21018501395230516
1101	4	0.12367366393473134
1101	5	0.22131700528967713
1101	6	0.18165224051800521
1101	7	0.23302972015711276
1101	8	0.27006694462682646
1101	9	0.26295733579036096
1101	10-11	0.13331673885800654
1101	12-13	0.17893455724897933
1101	14-15	0.11961100888068188
1101	16-17	0.1353483458400646
1101	18-19	0.16595041059091642
1101	20-21	0.24380514677683607
1101	22-23	0.2014204262810111
1101	24-25	0.13635679580306004
1101	26-27	0.16660126739405
1101	28-29	0.16802254936994387
1101	30-31	0.06075946574140545
1101	32-33	-0.005560939103574469
1101	34-35	0.02530978758304059
1101	36-37	0.02074990908668184
1101	38-39	-0.036852919281606944
1101	40-41	0.049460965324371386
1101	42-43	-0.0290721405763108
1101	44-45	0.06152814784947935
1101	46-47	-0.0032163463764760536
1101	48-49	-0.11446146531206125
1101	50-51	-0.1765550297868117
1101	52-53	-0.032047071940766614
1101	54-55	0.076812739109549
1101	56-57	0.0356019265360743
1101	58-59	-0.10030132057320174
1101	60-61	-0.054748679723232385
1101	62-63	-0.05739773838062234
1101	64-65	-0.09577247869977867
1101	66-67	-0.1306454824150265
1101	68-69	-0.0555121140676178
1101	70-71	-0.11885859669526155
1101	72-73	-0.18648512710951337
1101	74-75	-0.17393456090386294
1101	76-77	-0.1720447668304601
1101	78-79	-0.42087780155764776
1101	80-81	-0.19691330708036858
1101	82-83	-0.47579488465338216
1101	84-85	-0.37582720382020085
1101	86-87	-0.3919064876418723
1101	88-89	-0.5379803578777782
1101	90-91	-0.457772246607707
1101	92-93	-0.45790502777966324
1101	94-95	-0.5083253912252736
1101	96-97	-0.42538702315325594
1101	98-99	-0.478607949052698
1101	100	-0.4172419079565266
1102	1	0.0075929642762986305
1102	2	-0.07176376027793907
1102	3	0.06042186482059364
1102	4	-0.026308579958360667
1102	5	-0.0010583736825964252
1102	6	0.006152530347002028
1102	7	0.06256120922522967
1102	8	0.12692334106937153
1102	9	0.031142291670335
1102	10-11	0.10777369943328097
1102	12-13	-0.008454761808856404
1102	14-15	-0.032273396505566154
1102	16-17	0.06294074651650305
1102	18-19	0.11903425097386133
1102	20-21	-0.055724778883288195
1102	22-23	0.031080597247473918
1102	24-25	-0.02849414721126209
1102	26-27	0.032719178463338494
1102	28-29	0.02450010956841453
1102	30-31	-0.0023455840301025432
1102	32-33	0.033164649423405024
1102	34-35	-0.07705240036108307
1102	36-37	-0.452838017921394
1102	38-39	-0.21383768566638395
1102	40-41	-0.9581791483147128
1102	42-43	-0.8670059500175498
1102	44-45	-0.36249072295643003
1102	46-47	-0.27176712521482216
1102	48-49	-0.2804262004867155
1102	50-51	-0.45947302015099467
1102	52-53	-0.2678049149165673
1102	54-55	-0.14120473079575646
1102	56-57	-0.0019852035329392947
1102	58-59	-0.006755474994378119
1102	60-61	-0.23169794296268975
1102	62-63	-0.41855024211865555
1102	64-65	-0.14964340068971183
1102	66-67	-0.08744191798992063
1102	68-69	-0.13717025514288395
1102	70-71	-0.23923570629850843
1102	72-73	-0.2037597877846835
1102	74-75	-0.18169773397638878
1102	76-77	-0.2244335345239925
1102	78-79	-0.23690054787421033
1102	80-81	-0.3361963176600362
1102	82-83	-0.3981391224248867
1102	84-85	-0.4587169367639987
1102	86-87	-0.4406182539736818
1102	88-89	-0.3571108309846238
1102	90-91	-0.40283409916273527
1102	92-93	-0.33790979236524166
1102	94-95	-0.6126834635250908
1102	96-97	-0.6617093797423124
1102	98-99	-0.47673594029470934
1102	100	-0.5909006236114251
1103	1	0.05588846589954244
1103	2	-0.01592131268493091
1103	3	0.029738822616298677
1103	4	0.1249922734677682
1103	5	0.07444159242140103
1103	6	0.05455376868112438
1103	7	0.09183794375923782
1103	8	0.02123380213038928
1103	9	0.031187580501978118
1103	10-11	0.08730385369777593
1103	12-13	0.07492037659984163
1103	14-15	0.07152653419593236
1103	16-17	0.05154608589085541
1103	18-19	0.05360794277629566
1103	20-21	0.011539399063209999
1103	22-23	-0.06527807711734823
1103	24-25	-0.04180962864754889
1103	26-27	-0.13812102822697625
1103	28-29	-0.2260122379937144
1103	30-31	-0.2564165886324261
1103	32-33	-0.27804024064055
1103	34-35	-0.2999319697311478
1103	36-37	-0.36917160924100045
1103	38-39	-0.25908358236642215
1103	40-41	-0.2403322885914605
1103	42-43	-0.3012750956121977
1103	44-45	-0.24610642885961198
1103	46-47	-0.13199675183147974
1103	48-49	-0.2432208603591164
1103	50-51	-0.32285398678978794
1103	52-53	-0.1625558357580985
1103	54-55	-0.06092232056368374
1103	56-57	0.056111846384581554
1103	58-59	0.012973036820255857
1103	60-61	-0.01576978554962949
1103	62-63	-0.03209405082466077
1103	64-65	-0.15383313547228994
1103	66-67	-0.060877899731274
1103	68-69	-0.21451924843147196
1103	70-71	-0.15393405066686938
1103	72-73	-0.12981941462731328
1103	74-75	-0.18895039669605396
1103	76-77	-0.16983427006734786
1103	78-79	-0.2701829901191779
1103	80-81	-0.18413209790845642
1103	82-83	-0.1900950820961711
1103	84-85	-0.23893320270454055
1103	86-87	-0.7948295033051771
1103	88-89	-0.2498795886583025
1103	90-91	-0.6283101497506429
1103	92-93	-0.3422855785642973
1103	94-95	-0.6276698297978136
1103	96-97	-0.3747568944117532
1103	98-99	-0.34107235063224195
1103	100	-0.3619390277874146
1104	1	0.026854836989187447
1104	2	-0.049947861233178514
1104	3	0.014665518391122845
1104	4	0.06554308068524506
1104	5	-0.02240006033741082
1104	6	0.10755196487458818
1104	7	0.031548098459531104
1104	8	0.06370593022874971
1104	9	0.053963939397668526
1104	10-11	-0.05718796876495702
1104	12-13	0.026178826674858158
1104	14-15	-0.08598764057662578
1104	16-17	-0.009510903999959908
1104	18-19	0.026563031646084312
1104	20-21	-0.06329693780467949
1104	22-23	-0.054142978469926106
1104	24-25	-0.044506909451342835
1104	26-27	-0.02282295060148698
1104	28-29	-0.1015296031437245
1104	30-31	0.041129377527930444
1104	32-33	0.07806193572790221
1104	34-35	-0.04808429725971308
1104	36-37	-0.00668744874155891
1104	38-39	0.0013067085465578998
1104	40-41	-0.04424780610411716
1104	42-43	-0.32464007984996357
1104	44-45	-0.15127558489168536
1104	46-47	0.044919862856346526
1104	48-49	-0.013829947104788687
1104	50-51	-0.2610629010218801
1104	52-53	-0.1002867904269209
1104	54-55	0.07033263147528857
1104	56-57	0.1338351517336207
1104	58-59	0.005349495740105681
1104	60-61	-0.18517219034404775
1104	62-63	0.06271754692087228
1104	64-65	-0.11192104434915251
1104	66-67	0.025407902456898057
1104	68-69	-0.16197031807188722
1104	70-71	0.053411333330387833
1104	72-73	-0.02768870327569317
1104	74-75	-0.05238682375427928
1104	76-77	-0.1292830765205899
1104	78-79	-0.17200052890663997
1104	80-81	-0.24761193432961548
1104	82-83	-0.1111766144326296
1104	84-85	-0.09482848218216589
1104	86-87	-0.17517731906966105
1104	88-89	-0.1412942983915535
1104	90-91	-0.24438144787322358
1104	92-93	-0.23353515009000247
1104	94-95	-0.3094751254591266
1104	96-97	-0.2892823134736595
1104	98-99	-0.4194329236920247
1104	100	-0.2611317400718427
1105	1	0.16577924774339436
1105	2	0.2282276225048605
1105	3	0.22460148661403068
1105	4	0.092857012896161
1105	5	0.17566749733497034
1105	6	0.21296036877181024
1105	7	0.11950420861072786
1105	8	0.07572440923993895
1105	9	0.183321117691996
1105	10-11	0.1401724776466935
1105	12-13	0.22584049648084203
1105	14-15	0.12272663446540122
1105	16-17	0.0807345713057046
1105	18-19	0.10239666817432891
1105	20-21	0.08603630066273382
1105	22-23	0.13416203122529424
1105	24-25	0.1395103894894305
1105	26-27	0.1424167405560368
1105	28-29	0.004180693192672891
1105	30-31	0.07097554795523564
1105	32-33	0.032656429454448244
1105	34-35	0.01419609595272675
1105	36-37	-0.045869485703114776
1105	38-39	-0.04118114972575171
1105	40-41	0.018135528409381152
1105	42-43	-0.036514332310822795
1105	44-45	-0.09646506733285776
1105	46-47	-0.08675531252215762
1105	48-49	-0.023346054354739465
1105	50-51	-0.13420579532670018
1105	52-53	-0.03394876123059021
1105	54-55	0.09482082553852678
1105	56-57	0.07477655281988405
1105	58-59	0.07112836418903612
1105	60-61	0.0839334840830972
1105	62-63	0.042240235437326135
1105	64-65	-0.011545959274492645
1105	66-67	0.10394838893422076
1105	68-69	0.09166170110569993
1105	70-71	0.055186093912865886
1105	72-73	0.01783429810802062
1105	74-75	0.004869368002456298
1105	76-77	0.08593590179158284
1105	78-79	0.05421772006149084
1105	80-81	0.0958839332266912
1105	82-83	0.10064526696693576
1105	84-85	0.07809452162076269
1105	86-87	0.037434941765951635
1105	88-89	-0.09339483551658745
1105	90-91	-0.07153126974592183
1105	92-93	-0.07757398232587676
1105	94-95	0.030721195346966823
1105	96-97	0.04117286534701137
1105	98-99	-0.0027515786310168266
1105	100	-0.032853645749657545
1106	1	0.10558165381736728
1106	2	0.1083473400257482
1106	3	0.13755124637075156
1106	4	0.22572911043648958
1106	5	0.07357056328950051
1106	6	0.17316012207008669
1106	7	0.1469787235376927
1106	8	-0.9904329865291501
1106	9	0.1007192498524887
1106	10-11	0.0635649550494577
1106	12-13	0.1166858574957601
1106	14-15	0.08988646197614969
1106	16-17	0.01833139062906497
1106	18-19	0.09586718983626952
1106	20-21	0.009546035475651138
1106	22-23	0.06850510494481199
1106	24-25	0.11343850486144902
1106	26-27	0.03512837400793245
1106	28-29	-0.015525425423568606
1106	30-31	0.10280302294225407
1106	32-33	0.1488555286465285
1106	34-35	0.11289222633580209
1106	36-37	0.18593714740760703
1106	38-39	0.14297931716024692
1106	40-41	0.17849151709828703
1106	42-43	0.1163229866597959
1106	44-45	0.04774086045869552
1106	46-47	0.05755333844325605
1106	48-49	0.017793571398073027
1106	50-51	0.03406819395485172
1106	52-53	0.09416170193770768
1106	54-55	0.09560124845988582
1106	56-57	0.07539434528663946
1106	58-59	0.056843504784929166
1106	60-61	0.08538213217837409
1106	62-63	0.054764266649605986
1106	64-65	0.04225577293546223
1106	66-67	0.09517355686366358
1106	68-69	0.10356741530736713
1106	70-71	0.12618904014463084
1106	72-73	0.13555778734409074
1106	74-75	0.14616372763908103
1106	76-77	0.15011979961498412
1106	78-79	0.1495118447024666
1106	80-81	0.15949102950090221
1106	82-83	0.1283962930075262
1106	84-85	0.06958647474629842
1106	86-87	0.04897051377382411
1106	88-89	0.17602742837190632
1106	90-91	0.06296004007026923
1106	92-93	0.04973120765538752
1106	94-95	0.0028515677525682293
1106	96-97	0.05520455583955197
1106	98-99	0.05194904691814273
1106	100	0.23110554840933517
1107	1	-0.03128664781065993
1107	2	-0.05011716451494408
1107	3	-0.07506676433582982
1107	4	0.007513391076606979
1107	5	0.062350089153426325
1107	6	-3.1543046051751844E-4
1107	7	0.12519475626981347
1107	8	0.160793007878965
1107	9	0.12406690572134238
1107	10-11	0.04455028540886019
1107	12-13	0.0923593989924214
1107	14-15	0.003326349638676618
1107	16-17	0.10099367681807081
1107	18-19	0.1131962859108242
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2314	90-91	0.5565234081904045
2314	92-93	0.6212179919238032
2314	94-95	0.589912694173254
2314	96-97	0.6596979284579589
2314	98-99	0.6777666211291482
2314	100	0.6222743417929557
2315	1	-0.05616395361536419
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2315	10-11	-1.3283934471843608
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2315	26-27	0.008776500738683524
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2315	100	0.5279845862362293
2316	1	-0.11918007865824976
2316	2	-0.31141307202077684
2316	3	-0.2108211749329456
2316	4	-0.27027164222625544
2316	5	-0.23632488124830076
2316	6	-0.14389306524957846
2316	7	-0.09985705976372827
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2316	9	-0.024175361016880004
2316	10-11	-0.26426979915276405
2316	12-13	-0.21535165722058736
2316	14-15	-0.25751797764427664
2316	16-17	-0.3446791767994526
2316	18-19	-0.2033435858822159
2316	20-21	-0.06610665062584786
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2316	32-33	-0.1108336419823317
2316	34-35	-0.10612491308807392
2316	36-37	-0.021902478033702266
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2316	54-55	0.008702484141764444
2316	56-57	-0.13991505831168638
2316	58-59	-0.1900823655631001
2316	60-61	-0.09728515637952029
2316	62-63	-0.08942295748152418
2316	64-65	-0.29665242291522986
2316	66-67	-0.29097049232385785
2316	68-69	-0.048486714657002494
2316	70-71	0.024900232334083228
2316	72-73	-0.010708885570544169
2316	74-75	0.009340393059787289
2316	76-77	-0.18894613938082827
2316	78-79	-0.2252194833949197
2316	80-81	0.03050599448501501
2316	82-83	0.008535749935198567
2316	84-85	0.07268203639726067
2316	86-87	0.06558639750051043
2316	88-89	0.11605606370208221
2316	90-91	0.20319124411634348
2316	92-93	0.46491198293434266
2316	94-95	0.41181106962932645
2316	96-97	0.31176701576807275
2316	98-99	0.41210788709100754
2316	100	-0.0485291849420868
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	12089.0
1	1095.0
2	666.0
3	572.0
4	470.0
5	398.0
6	497.0
7	1093.0
8	2058.0
9	3340.0
10	5995.0
11	11271.0
12	18011.0
13	23512.0
14	27003.0
15	30330.0
16	34448.0
17	39892.0
18	46455.0
19	54202.0
20	61805.0
21	71137.0
22	80441.0
23	86875.0
24	91896.0
25	98504.0
26	107721.0
27	121965.0
28	140259.0
29	164942.0
30	197500.0
31	242735.0
32	306131.0
33	404388.0
34	576697.0
35	897795.0
36	921514.0
37	925656.0
38	819894.0
39	165809.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72763477390462	17.737306191171523	12.863807073759807	23.671251961164053
2	15.996202187437566	20.442981945373393	39.350658853345045	24.210157013843993
3	19.759827051392413	26.05251650761048	28.91462765474681	25.273028786250297
4	13.121675069274847	16.17857850684499	37.17852700357675	33.52121942030342
5	13.709141498670549	36.81230159122528	33.597103584230396	15.881453325873782
6	30.836730461583667	35.320998751280655	18.765467024870844	15.07680376226484
7	28.166958027001815	31.502758532393504	22.15742249057922	18.172860950025456
8	26.656342748954753	33.0766612102023	20.588280411332256	19.67871562951069
9	27.40069431885267	16.185707133393066	20.35625218873884	36.05734635901542
10-11	25.660571031636888	25.234026518556423	27.94662220606486	21.15878024374183
12-13	26.534838901088836	23.105083418800973	27.59843073374783	22.76164694636236
14-15	23.719869197226846	24.45963639731007	26.032562379081053	25.78793202638203
16-17	23.108657635870248	27.042176380793197	26.566894202507118	23.282271780829433
18-19	22.9444553380465	26.746229865261917	27.799630941641567	22.509683855050017
20-21	23.91472423457325	25.71794939111284	27.489980197856244	22.87734617645766
22-23	23.547839098333384	25.680543036340854	27.299250459540385	23.47236740578537
24-25	23.631627633973984	25.756725198807356	27.299195258339882	23.312451908878778
26-27	23.313267954944774	25.422625004534837	27.94502859087835	23.319078449642035
28-29	23.05307942430059	26.252414062046004	27.592172406581295	23.102334107072114
30-31	23.755075989890383	25.66731206661961	27.408328763624425	23.169283179865584
32-33	23.536273437260906	25.888931783379626	27.471008424895864	23.1037863544636
34-35	23.864202928791624	25.86305418065428	27.4047803553746	22.86796253517949
36-37	23.814032727836622	25.6636608252947	27.506084695899407	23.016221750969272
38-39	23.57193816358577	26.10437890565519	27.369880317212296	22.953802613546745
40-41	23.55366235317137	26.018401614464988	27.328646184847038	23.099289847516605
42-43	23.356771428830577	26.06781623528286	26.857567942590542	23.717844393296026
44-45	23.441894106795665	25.839993039841747	27.020241231417153	23.69787162194544
46-47	23.720612736228432	25.962535837766882	26.933241255968294	23.3836101700364
48-49	23.605591989195634	26.024517768911785	26.992722243668233	23.377167998224344
50-51	23.012024837601686	26.32116859687518	27.327408080958083	23.339398484565056
52-53	23.275746490061966	26.565609576335447	26.54261535055575	23.616028583046837
54-55	23.036728799571048	26.17236183461825	26.90971835525428	23.881191010556424
56-57	23.48092496402958	25.98363588264287	26.749389725258915	23.786049428068633
58-59	23.78012013983963	25.931389077440137	26.813359045135186	23.47513173758504
60-61	23.648183019532656	26.202451171309022	27.213061253622804	22.936304555535518
62-63	23.18295633059039	26.219029950729077	27.248591102970792	23.34942261570974
64-65	23.10442071608028	26.452293740503684	26.88287205017757	23.560413493238467
66-67	23.31670896513677	26.03485954474013	26.62821083003096	24.020220660092146
68-69	23.01437285940354	26.157254342412088	26.83565124103425	23.992721557150123
70-71	23.40386102619228	25.932532704846682	26.778502056163212	23.885104212797824
72-73	23.189554303871724	25.924220244446015	26.978807689132324	23.90741776254994
74-75	22.95383808742469	26.583710724326632	26.609137314949077	23.8533138732996
76-77	22.996791118665026	25.912319786714832	26.798706592822736	24.292182501797402
78-79	23.08479733280717	25.955356237310827	26.90290304318883	24.05694338669317
80-81	23.250174088364762	26.03799957018132	26.627318738280103	24.08450760317381
82-83	23.16852648343277	25.877463045103532	26.949112262281076	24.004898209182624
84-85	22.983643542019177	26.262282244242886	26.669906474880644	24.084167738857296
86-87	22.923104452898748	26.268955987338504	26.716293463770068	24.091646095992676
88-89	22.758814314229287	26.014414138753327	26.660129963449393	24.566641583567993
90-91	23.287856549487444	25.770921698192677	26.62485245579501	24.31636929652487
92-93	23.278238661798316	26.026797072794437	26.70351608333229	23.991448182074958
94-95	22.985681378888316	25.7646811673354	26.849325051789158	24.400312401987122
96-97	22.67902625120878	25.727438557979625	27.204215653949337	24.38931953686226
98-99	22.969292043641502	25.804043962469308	26.695935940073145	24.530728053816052
100	23.001958434680432	25.20365566604926	26.76649362291531	25.02789227635499
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3969.0
1	5069.5
2	4850.0
3	2492.0
4	1272.5
5	1145.0
6	1435.0
7	2310.5
8	3646.0
9	4686.5
10	4822.0
11	4755.0
12	5046.0
13	5527.5
14	6001.5
15	6324.0
16	6697.5
17	7145.5
18	7633.5
19	8157.0
20	8886.5
21	9849.0
22	11341.5
23	13254.0
24	15447.5
25	18660.0
26	22932.5
27	27656.5
28	33216.0
29	40765.0
30	49929.5
31	59086.5
32	70385.0
33	83801.5
34	96885.5
35	110745.5
36	130388.5
37	147807.5
38	157766.5
39	168860.5
40	177208.5
41	183979.0
42	192496.5
43	203446.5
44	215676.5
45	228421.5
46	241841.0
47	268523.0
48	317627.5
49	330925.0
50	312018.5
51	293349.5
52	271239.0
53	261083.0
54	253027.0
55	239329.0
56	220566.0
57	199989.5
58	180654.0
59	160173.0
60	136833.0
61	114402.0
62	94683.0
63	75646.0
64	57991.5
65	44037.5
66	33319.5
67	26046.5
68	20755.5
69	16337.5
70	12965.5
71	10324.0
72	8315.5
73	6704.0
74	5311.5
75	3956.5
76	2871.5
77	1957.5
78	1286.0
79	890.5
80	612.5
81	418.0
82	302.5
83	222.0
84	167.5
85	137.0
86	104.0
87	79.0
88	61.5
89	51.0
90	44.0
91	32.0
92	23.5
93	23.5
94	22.0
95	13.5
96	6.0
97	5.0
98	4.0
99	2.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14394147478515806
2	0.07173534659162212
3	0.3102744995113434
4	0.3080382869022526
5	0.3105393141624199
6	0.2568849234693014
7	0.25767936742253106
8	0.2571791619704976
9	0.2675952049128414
10-11	0.2452772145971132
12-13	0.2552592557501927
14-15	0.2287189429422997
16-17	0.23653097514905755
18-19	0.24978641962794426
20-21	0.2520594120496845
22-23	0.24302629006296264
24-25	0.24215093052190406
26-27	0.2408783490042307
28-29	0.25217710745016303
30-31	0.23745782642782542
32-33	0.2501689296794992
34-35	0.23409615155165936
36-37	0.2422392020722629
38-39	0.24384280190378194
40-41	0.2610998899989363
42-43	0.24600545488757367
44-45	0.23174224354209016
46-47	0.2346993404791115
48-49	0.24443127890617428
50-51	0.23711945215144983
52-53	0.25369243573132316
54-55	0.2636671209218728
56-57	0.2600847671698096
58-59	0.2565906849681053
60-61	0.2383920336691232
62-63	0.2656458748424169
64-65	0.2589887287528539
66-67	0.2648440749266574
68-69	0.26900019375605305
70-71	0.28918495493810914
72-73	0.298527027351087
74-75	0.2776140258785704
76-77	0.27476726837949766
78-79	0.272825294271603
80-81	0.2589887287528539
82-83	0.2707435568756402
84-85	0.27993851003802006
86-87	0.27281793830907314
88-89	0.28056376685306184
90-91	0.2755543563701974
92-93	0.25542844288838046
94-95	0.2760104260470514
96-97	0.27455394546613043
98-99	0.2610263303736373
100	0.28460219028197903
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6797207.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.463476130611156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.21802046807812	40.0546245443124
2	16.19988498038897	17.970038678568997
3	5.574065157386817	9.27471089421585
4	2.287052753363449	5.0739158318248885
5	1.1188185765402308	3.102678370721172
6	0.6372390395450067	2.120609535557884
7	0.42360059165749525	1.6446052913015776
8	0.27345138291898424	1.2133251399527754
9	0.2073101370903332	1.0348326756129078
>10	0.9637811350322552	9.414532265292246
>50	0.05964299004789433	2.2764569309745966
>100	0.034131733512236764	3.4294853575343587
>500	0.0019292507281487082	0.7387361679552105
>1k	8.217161778392205E-4	0.9328656270882906
>5k	1.429071613614677E-4	0.5432568085322862
>10k+	1.0718037102110078E-4	1.1753258805545674
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	28759	0.4231002527950083	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17281	0.254236776958536	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13274	0.1952860932438868	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	7927	0.11662142994909527	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	7322	0.10772071528791165	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7098	0.10442524407451473	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	2.9423850119615307E-5
5	0.0	0.0	0.0	0.0	2.9423850119615307E-5
6	0.0	0.0	0.0	0.0	5.8847700239230614E-5
7	0.0	0.0	0.0	0.0	5.8847700239230614E-5
8	0.0	0.0	0.0	0.0	7.355962529903826E-5
9	0.0	0.0	0.0	0.0	1.1769540047846123E-4
10-11	0.0	0.0	0.0	0.0	1.1769540047846123E-4
12-13	0.0	0.0	0.0	7.355962529903827E-6	1.324073255382689E-4
14-15	0.0	0.0	0.0	1.4711925059807654E-5	1.986109883074033E-4
16-17	0.0	0.0	0.0	3.677981264951914E-5	3.089504262559607E-4
18-19	0.0	0.0	0.0	5.8847700239230614E-5	3.6779812649519135E-4
20-21	0.0	0.0	0.0	9.562751288874974E-5	3.82510051554999E-4
22-23	0.0	0.0	0.0	1.7654310071769184E-4	4.119339016746143E-4
24-25	0.0	0.0	0.0	3.310183138456722E-4	4.413577517942296E-4
26-27	0.0	0.0	0.0	7.061724028707674E-4	5.002054520334602E-4
28-29	0.0	0.0	0.0	0.0021332291336721098	5.002054520334602E-4
30-31	0.0	0.0	0.0	0.008069490895304498	5.002054520334602E-4
32-33	0.0	0.0	0.0	0.02508383222697205	5.149173770932679E-4
34-35	0.0	0.0	0.0	0.05375737416853717	5.149173770932679E-4
36-37	0.0	0.0	0.0	0.09794464108566944	5.149173770932679E-4
38-39	0.0	0.0	0.0	0.16959171612693272	5.149173770932679E-4
40-41	0.0	0.0	0.0	0.255009153024176	5.149173770932679E-4
42-43	0.0	0.0	0.0	0.3421331732283569	5.149173770932679E-4
44-45	0.0	0.0	0.0	0.4479119144083739	5.222733396231718E-4
46-47	0.0	0.0	0.0	0.5737577213699685	5.443412272128832E-4
48-49	0.0	0.0	0.0	0.7048924653905642	5.590531522726909E-4
50-51	2.9423850119615307E-5	0.0	0.0	0.8436332746670802	5.590531522726909E-4
52-53	5.8847700239230614E-5	0.0	0.0	0.9894505198973638	5.884770023923062E-4
54-55	5.8847700239230614E-5	0.0	0.0	1.1600205790407738	6.179008525119214E-4
56-57	5.8847700239230614E-5	0.0	0.0	1.3576752922192894	6.179008525119214E-4
58-59	5.8847700239230614E-5	0.0	0.0	1.5806492284257343	6.326127775717291E-4
60-61	6.620366276913444E-5	0.0	0.0	1.8048883901873225	7.20884327930575E-4
62-63	1.0298347541865357E-4	0.0	0.0	2.0426919468540534	7.65020103109998E-4
64-65	1.0298347541865357E-4	0.0	0.0	2.281517982312441	7.944439532296132E-4
66-67	1.0298347541865357E-4	0.0	0.0	2.551231704433895	8.165118408193248E-4
68-69	1.0298347541865357E-4	0.0	0.0	2.8591522959356688	8.385797284090362E-4
70-71	1.0298347541865357E-4	0.0	0.0	3.18413872050682	8.827155035884592E-4
72-73	1.0298347541865357E-4	7.355962529903827E-6	0.0	3.501268977096034	0.001015122829126728
74-75	1.0298347541865357E-4	1.4711925059807654E-5	0.0	3.83581079699353	0.0010445466792463433
76-77	1.0298347541865357E-4	1.4711925059807654E-5	0.0	4.189279508480468	0.0010886824544257662
78-79	1.0298347541865357E-4	1.4711925059807654E-5	0.0	4.592202356055951	0.001103394379485574
80-81	1.0298347541865357E-4	1.4711925059807654E-5	0.0	5.02054005417225	0.0011181063045453818
82-83	1.0298347541865357E-4	1.4711925059807654E-5	0.0	5.452585157403622	0.0012210897799640353
84-85	1.0298347541865357E-4	1.4711925059807654E-5	0.0	5.895399389778772	0.0012210897799640353
86-87	1.0298347541865357E-4	1.4711925059807654E-5	0.0	6.355382438698719	0.0012210897799640353
88	1.0298347541865357E-4	2.9423850119615307E-5	0.0	6.714552021146333	0.0012210897799640353
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	11760	0.0	50.459335	2
GTACCTG	13250	0.0	45.363556	1
ACCTGGG	13295	0.0	43.643494	3
CCTGGGG	12460	0.0	32.301746	4
TATAACG	645	0.0	26.973139	2
TATCACG	670	0.0	25.264875	2
CTGGGGG	7565	0.0	22.06232	5
CATGGGG	17605	0.0	20.912018	4
GTACATG	33400	0.0	20.31945	1
GTACCCG	1165	0.0	20.185345	1
GAGTACT	15895	0.0	19.76272	12-13
TACATGG	34635	0.0	19.359486	2
ATGGGGG	10400	0.0	19.167448	5
ACATGGG	34445	0.0	18.893072	3
GTACACG	1520	0.0	18.874624	1
TAACGCA	875	0.0	17.732752	4
TGGGGGG	9950	0.0	17.710142	6
AGTACTT	17645	0.0	17.21681	12-13
GTACTTT	18235	0.0	17.188084	14-15
GTATAGG	1940	0.0	16.485392	1
>>END_MODULE