FastQCFastQC Report
Mon 6 Jun 2016
ERR522864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6797207
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT297400.43753265127867963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150750.22178227027660039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128500.18904823701852835No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT72320.10639664203252895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72190.10620538700675144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG119150.049.978022
GTACCTG139300.043.6171341
ACCTGGG137650.042.5035973
CCTGGGG120250.032.553114
TATAACG6750.026.4591732
TAACGCA7400.025.4015874
CTGGGGG70800.022.8325585
GTACCCG11000.021.3758341
ATAACGC8900.021.1204193
TGGGGGG88000.019.8649026
CATGGGG180550.019.781024
GAGTACT167600.019.66051112-13
TATCACG6050.019.4214252
GTACATG348450.019.1778261
TACATGG358800.018.2602522
ATGGGGG100850.018.0328525
GTACTTT182800.017.7666814-15
ACATGGG354850.017.7456763
GTACACG15500.017.2937411
AGTACTT183800.016.64892612-13
Produced by FastQC (version 0.11.5)