FastQCFastQC Report
Mon 6 Jun 2016
ERR522863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8348932
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT893991.0707836643058057No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT256550.30728481199751057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242170.29006105212019934No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC200300.23991092513389733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193580.23186199144992437No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC182500.21859083293527845No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG146720.17573505210007698No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA120640.1444975237551342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115590.13844884591226758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG324250.027.8718241
TACATGG333400.026.596772
ACATGGG337850.025.286673
CATGGGG226850.023.3362754
ATGGGGG123250.020.6361735
GAGTACT218850.020.447912-13
AGAGTAC366950.019.03662910-11
GAGTACA255200.019.0147691
GTACTTT235650.018.82077414-15
AGTACTT231500.018.11288312-13
AGTACAT247550.018.0431982
CATGGGA117550.017.3179784
ACTTTTT290800.015.51749216-17
TACTTTT271800.015.34957914-15
GTATTAT29500.014.6641811
ATGGGGA94050.014.4963295
ATGGGAG62900.014.3505795
TACCTGG54950.014.1178662
GTATATA35700.014.0931331
GTATAGG22050.014.0743181
Produced by FastQC (version 0.11.5)