FastQCFastQC Report
Mon 6 Jun 2016
ERR522863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8348932
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT903711.0824258719558382No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT252470.3023979594036698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221810.26567469947054306No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC206750.247636464160925No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC189360.22680745273766756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186890.22384899050561197No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA153210.1835085014466521No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG149570.1791486623678334No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA128330.15370828268813305No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC121060.1450005821103825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106930.12807626173024286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG313450.027.2918761
TACATGG323400.025.8696962
ACATGGG331150.024.6506983
CATGGGG226300.022.3866124
GAGTACT213000.020.84622212-13
GTACTTT225700.019.45171514-15
ATGGGGG121300.019.255165
GTATAGG22000.018.8117391
AGAGTAC358750.018.6276210-11
GAGTACA249150.018.2907641
AGTACTT224150.017.92166512-13
AGTACAT241600.017.6644572
CATGGGA109250.016.2171334
ACTTTTT275650.016.18850316-17
TACTTTT259100.015.29335414-15
TATACTG35400.015.1339795
ATGGGGA92450.014.7923645
GTATACG5351.4006218E-1014.0648521
GTATATA33100.013.7820141
CATGGGT58100.013.7507684
Produced by FastQC (version 0.11.5)