FastQCFastQC Report
Mon 6 Jun 2016
ERR522862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7085524
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT541750.7645870651203779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287720.40606735648626696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234490.33094235514550513No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT151210.21340694068639104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135760.19160191963219658No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC121520.1715046057285248No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC105430.148796334611244No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA90720.12803569644249316No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG85480.1206403365509735No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA81650.115234949454691No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG55400.031.3976962
GTACATG290650.030.2153571
TACATGG298250.029.0503042
ACATGGG307600.027.6186373
CATGGGG224500.026.803614
ATGGGGG152650.026.7936175
GTACCTG71800.025.0126741
ACCTGGG71650.023.5563873
TGGGGGG155550.023.2566156
GTATACG4000.021.1560081
GAGTACT222600.021.14795312-13
TATCACG6250.021.0612342
GTGGTTA49450.019.9652231
AGAGTAC357200.019.36437610-11
GTACTTT244350.019.07286514-15
CGTATAC4800.018.6098483
AGTACTT234600.018.50417712-13
TATAACG6100.018.4963992
GGTTAAG65600.017.0570353
TGGTTAA60200.016.3994242
Produced by FastQC (version 0.11.5)