FastQCFastQC Report
Mon 6 Jun 2016
ERR522862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7085524
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT553490.7811560584651185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266990.37681052241160995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228080.32189574123240566No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT148950.21021733890111727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130930.1847852043123416No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC127580.18005725476337386No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC107280.15140729182485305No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA93030.13129586463894555No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG87560.12357589925600422No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA84280.11894674268268655No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC74320.10488991357590491No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG56350.032.948112
GTACATG292350.029.1500931
TACATGG302900.027.8387952
ATGGGGG153100.027.4420195
ACATGGG306600.026.8086193
GTACCTG72150.026.6607171
CATGGGG221200.026.6421474
ACCTGGG72050.025.3730343
TGGGGGG150050.024.7110776
GAGTACT232000.021.04312312-13
GTGGTTA47600.020.1561721
AGAGTAC366050.019.67478810-11
AGTACTT245200.019.04754812-13
TATAACG5550.018.6318932
GTACTTT258750.018.62949214-15
TATCACG6250.018.0492252
CCTGGGG75600.017.219274
GTATATA31150.016.3061181
ATAACGC7500.016.2917943
TACTTTT289200.016.10723714-15
Produced by FastQC (version 0.11.5)