FastQCFastQC Report
Mon 6 Jun 2016
ERR522861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10601684
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256920.24233885861906468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208040.19623297581780405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110320.10405894007027564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144900.052.5003551
GTATCAA261050.040.9814531
TCAACGC311750.033.4494324
CAACGCA317650.032.7826775
ATCAACG318600.032.7620773
TATCAAC328400.032.0033072
AACGCAG332350.031.425276
ACGCAGA381100.027.3931777
GTACATG347850.026.2379761
CGCAGAG403400.025.8314388
TACATGG354750.025.3862932
ACATGGG367850.023.8912853
GTGGTAT73900.023.8093621
GCAGAGT451400.022.667039
CATGGGG273150.022.5032064
TGGTATC77450.021.6050222
GAGTACT249900.020.5018312-13
CAGAGTA432600.020.15513210-11
AGAGTAC405900.019.06201210-11
ATGGGGG153500.018.7967825
Produced by FastQC (version 0.11.5)