FastQCFastQC Report
Mon 6 Jun 2016
ERR522861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10601684
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222540.20991004825271156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196430.18528188540612983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142100.051.479841
GTATCAA250550.039.6364481
TCAACGC303000.032.243294
ATCAACG310300.031.4698963
CAACGCA310550.031.3835935
TATCAAC321850.030.494982
AACGCAG325650.030.057966
ACGCAGA380750.025.6833387
GTACATG341000.024.9979671
CGCAGAG400550.024.3485368
TACATGG349050.024.0766642
ACATGGG361900.022.6069373
GCAGAGT458500.021.1174459
GTGGTAT76150.021.0661581
CATGGGG266500.020.5779024
GAGTACT250150.019.9733712-13
CAGAGTA439100.018.9892510-11
AGAGTAC409500.018.96728310-11
TGGTATC80300.018.858182
ATGGGGG146850.018.2400635
Produced by FastQC (version 0.11.5)