FastQCFastQC Report
Mon 6 Jun 2016
ERR522860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14823859
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT459610.3100474714445139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361230.24368148671678544No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT343800.23192341481391587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234750.1583595742512122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG247250.053.217162
GTACCTG293200.045.4988251
ACCTGGG289550.044.9556473
CCTGGGG260800.033.5350234
CATGGGG413100.025.7927674
GTACATG687750.025.2516081
TGGGGGG262300.025.1178676
ATGGGGG256750.024.4110495
TACATGG706050.024.2174662
TATAACG13550.023.2528292
ACATGGG722050.023.1598763
CTGGGGG172500.022.705065
TATCACG14450.021.4791152
GTACCCG29950.021.202881
TTAAGAG129050.018.9457635
GAGTACT394950.018.7090712-13
GTACACG34150.017.631011
GTATCAA978100.017.6161751
ATAACGC18550.017.49233
AGTACTT419300.017.41525312-13
Produced by FastQC (version 0.11.5)