##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522860_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14823859
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08994102008121	33.0	31.0	34.0	27.0	34.0
2	31.42051324152503	33.0	31.0	34.0	27.0	35.0
3	31.374578508875455	33.0	31.0	34.0	26.0	36.0
4	33.67066085828258	35.0	33.0	37.0	30.0	37.0
5	33.747871792358524	35.0	34.0	37.0	30.0	37.0
6	33.97907582634185	35.0	35.0	37.0	29.0	37.0
7	33.8503198795941	35.0	34.0	37.0	30.0	37.0
8	33.935492775531664	35.0	34.0	37.0	30.0	37.0
9	35.080630691373955	37.0	34.0	39.0	29.0	39.0
10-11	35.03393154913306	37.0	34.5	39.0	29.5	39.0
12-13	35.054281513336036	37.0	35.0	39.0	29.0	39.0
14-15	35.7853762977643	38.0	35.0	40.0	29.0	41.0
16-17	35.94189117691958	38.0	35.5	40.0	28.5	41.0
18-19	35.789926766033055	38.0	34.0	40.0	28.0	41.0
20-21	35.879689425000606	38.0	35.0	40.0	28.0	41.0
22-23	35.73302403915201	38.0	35.0	40.0	28.5	41.0
24-25	35.696636516847605	37.5	34.5	40.0	27.5	41.0
26-27	35.57751470113146	37.0	35.0	40.0	27.0	41.0
28-29	35.33604242323136	37.0	34.5	40.0	27.0	41.0
30-31	35.158182730960945	37.0	34.5	40.0	26.5	41.0
32-33	35.121007087290835	37.5	34.0	40.0	26.0	41.0
34-35	34.96661743072435	37.5	34.0	40.0	25.0	41.0
36-37	34.79641286388382	37.5	34.0	40.0	25.0	41.0
38-39	34.629218478130426	36.5	33.5	40.0	24.5	41.0
40-41	34.58016117800365	37.0	33.0	40.0	24.0	41.0
42-43	34.38011084023398	36.0	33.0	40.0	24.0	41.0
44-45	34.289625731059644	37.0	33.0	40.0	24.0	41.0
46-47	34.0817545215453	36.5	33.0	40.0	23.0	41.0
48-49	33.93919265556964	37.0	33.0	39.0	22.0	41.0
50-51	32.93130985663045	35.5	31.5	38.5	21.0	40.0
52-53	33.17578260154795	36.0	31.5	38.5	21.0	39.5
54-55	34.03311715255791	37.0	33.0	39.5	22.0	40.5
56-57	34.17165995710024	36.5	33.0	40.0	22.5	41.0
58-59	34.09123541987279	37.0	33.0	40.0	22.0	41.0
60-61	33.8305782927374	36.5	33.0	39.0	22.0	41.0
62-63	33.64508330792947	36.0	32.0	39.0	21.5	41.0
64-65	33.25097027703785	36.0	32.0	39.0	21.0	41.0
66-67	33.045954093330224	36.0	32.0	38.0	20.5	40.0
68-69	32.694646043246905	35.0	31.5	37.5	20.0	40.0
70-71	32.471863466861095	35.0	31.0	37.0	19.5	39.5
72-73	32.14194144723044	35.0	31.0	37.0	19.5	39.0
74-75	31.773182947841043	35.0	31.0	36.0	18.5	39.0
76-77	31.642441451986286	35.0	30.0	36.5	18.0	38.0
78-79	31.36940472113233	35.0	30.0	36.0	18.0	37.5
80-81	31.09664740470076	34.5	29.5	36.0	18.0	37.5
82-83	30.78605827268055	34.0	29.0	35.5	17.5	37.0
84-85	30.546740393307843	34.0	29.0	35.0	17.5	37.0
86-87	30.305883575929855	34.0	29.0	35.0	17.0	37.0
88-89	30.14195615325267	34.0	29.0	35.0	17.0	36.5
90-91	29.917428079962175	34.0	29.0	35.0	17.0	36.0
92-93	29.654975199103013	34.0	27.0	35.0	15.0	36.0
94-95	29.54510991368712	34.0	27.0	35.0	15.0	36.0
96-97	29.3454759317395	34.0	27.0	35.0	13.0	36.0
98-99	29.25375126679227	34.0	26.0	35.0	11.0	36.0
100	27.99127001950032	32.0	24.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1417533252128429
1101	2	0.11230276294939046
1101	3	0.1855785804622201
1101	4	0.17950673476777723
1101	5	0.1542639127635539
1101	6	0.19602044443953304
1101	7	0.19331583739137415
1101	8	0.20686104900772762
1101	9	0.2539920045609776
1101	10-11	0.16194044493861526
1101	12-13	0.17123640099703863
1101	14-15	0.09073340963523435
1101	16-17	0.1586405809519249
1101	18-19	0.17571028379529707
1101	20-21	0.18138169155510298
1101	22-23	0.12434607474524029
1101	24-25	0.014444669610604421
1101	26-27	0.05918034213499368
1101	28-29	-0.003430112090299531
1101	30-31	-0.03133371956239017
1101	32-33	-0.03267801923691138
1101	34-35	-0.024506521489968236
1101	36-37	-0.05633849184212636
1101	38-39	-0.049855891832578436
1101	40-41	-0.027755715825726668
1101	42-43	-0.0889998977381552
1101	44-45	-0.06866505056655114
1101	46-47	-0.08478519788759797
1101	48-49	-0.15678726716751612
1101	50-51	-0.1756386546601476
1101	52-53	-0.09692921699964785
1101	54-55	-0.008621625790382836
1101	56-57	-0.04021774955070612
1101	58-59	-0.02840158177284735
1101	60-61	-0.10884043766895957
1101	62-63	-0.12040504256827944
1101	64-65	-0.09639400622431538
1101	66-67	-0.1337186788067939
1101	68-69	-0.06494726799364514
1101	70-71	-0.1727344718881838
1101	72-73	-0.15208090141317143
1101	74-75	-0.18749442815624917
1101	76-77	-0.12635922747540818
1101	78-79	-0.3358733768985438
1101	80-81	-0.2909816098196423
1101	82-83	-0.47722690181641525
1101	84-85	-0.392974626630906
1101	86-87	-0.3131632447590853
1101	88-89	-0.44558875058807246
1101	90-91	-0.4470177297618001
1101	92-93	-0.4170618641991517
1101	94-95	-0.43570744629822755
1101	96-97	-0.39885309161239846
1101	98-99	-0.3707270067835964
1101	100	-0.44086952965186654
1102	1	0.06608215089339353
1102	2	0.03994836216281783
1102	3	0.06124410766840782
1102	4	0.015756228532126215
1102	5	0.03093312857926378
1102	6	0.06747942178373734
1102	7	0.140282209390989
1102	8	0.16749845719279932
1102	9	0.07546954264476113
1102	10-11	0.0922147925763781
1102	12-13	0.04445368145413653
1102	14-15	-0.03484729141206344
1102	16-17	0.08370155070929997
1102	18-19	0.11583596269485241
1102	20-21	-0.02864124304524296
1102	22-23	-0.028387259000304255
1102	24-25	-0.04525318756269314
1102	26-27	-0.04766501181653382
1102	28-29	0.03421923692484086
1102	30-31	-0.11559527702858219
1102	32-33	-0.07692736378137255
1102	34-35	-0.11793334903185837
1102	36-37	-0.5864748077470452
1102	38-39	-0.2390398388585453
1102	40-41	-0.8977822411070449
1102	42-43	-0.8893540330941576
1102	44-45	-0.3292962931819545
1102	46-47	-0.24992008070254457
1102	48-49	-0.3652862576779299
1102	50-51	-0.43092604279075175
1102	52-53	-0.20060778184119954
1102	54-55	-0.052991842355027075
1102	56-57	0.06865491949434954
1102	58-59	-0.005700336991012023
1102	60-61	-0.0788269218686537
1102	62-63	-0.3273386865409478
1102	64-65	-0.15163434718303392
1102	66-67	-0.1047223768775396
1102	68-69	-0.11974115108338168
1102	70-71	-0.19879334552390304
1102	72-73	-0.14321064498233582
1102	74-75	-0.1455573347043213
1102	76-77	-0.31838999238268073
1102	78-79	-0.26005120513537605
1102	80-81	-0.30972282956915365
1102	82-83	-0.34840067796039165
1102	84-85	-0.4565494148476681
1102	86-87	-0.3896972022253813
1102	88-89	-0.378113497707254
1102	90-91	-0.3680881237857143
1102	92-93	-0.4300055060374959
1102	94-95	-0.5687707778849891
1102	96-97	-0.7470915387095758
1102	98-99	-0.6287334813819605
1102	100	-0.6564919488080996
1103	1	0.025161365366301425
1103	2	0.027758339388249453
1103	3	0.06346483446962736
1103	4	-0.023254462663700792
1103	5	0.06374835973489468
1103	6	0.14089533322287906
1103	7	0.11014750708221754
1103	8	0.10072950585811213
1103	9	0.12016714430345132
1103	10-11	0.05123602622419554
1103	12-13	0.14363061450530523
1103	14-15	0.11661168868420191
1103	16-17	0.04747197005781345
1103	18-19	0.03919760525251803
1103	20-21	-0.04346783673961596
1103	22-23	-0.10517627611755387
1103	24-25	-0.11492876369226224
1103	26-27	-0.12706755653348978
1103	28-29	-0.2650007493502784
1103	30-31	-0.2706229117169201
1103	32-33	-0.25229549146371966
1103	34-35	-0.3023151401257991
1103	36-37	-0.43287239974670655
1103	38-39	-0.3084178474952637
1103	40-41	-0.2780446262929104
1103	42-43	-0.22652588863223144
1103	44-45	-0.22577558785413032
1103	46-47	-0.1299049082993946
1103	48-49	-0.22619510112411945
1103	50-51	-0.3086420346686509
1103	52-53	-0.022616387696373863
1103	54-55	-0.00685980732385616
1103	56-57	0.09822966380263409
1103	58-59	0.10543804825763914
1103	60-61	0.09892661518784962
1103	62-63	0.06353493468488125
1103	64-65	-0.1033275123373869
1103	66-67	-0.015320042371023135
1103	68-69	-0.07388254925944437
1103	70-71	-0.005772225230053607
1103	72-73	-0.0341215452976229
1103	74-75	0.02725410202007339
1103	76-77	0.04848725723475056
1103	78-79	-0.0994990807993652
1103	80-81	-0.01607882119160209
1103	82-83	-0.05117433800862159
1103	84-85	-0.053328352508039245
1103	86-87	-0.7663741425940067
1103	88-89	-0.2380373057409635
1103	90-91	-0.5119860211226666
1103	92-93	-0.17160072111602176
1103	94-95	-0.5055025751921178
1103	96-97	-0.22873716171607228
1103	98-99	-0.17940603500491292
1103	100	-0.26584663207021464
1104	1	0.1352038222411558
1104	2	0.019025996483893692
1104	3	0.05355973466877728
1104	4	0.10619014378571734
1104	5	0.0033214592719090774
1104	6	0.16804007656650555
1104	7	0.04335875809618983
1104	8	0.15683631226563932
1104	9	0.15531533835300593
1104	10-11	0.009930297959776624
1104	12-13	0.13002535727034115
1104	14-15	-0.03574076094672307
1104	16-17	0.06051291478981824
1104	18-19	0.10625778489288251
1104	20-21	0.04798614777270416
1104	22-23	0.04887730529168266
1104	24-25	0.046280324266021466
1104	26-27	0.08582680985927738
1104	28-29	0.0789633069533906
1104	30-31	0.03336944772351558
1104	32-33	0.12204334020270124
1104	34-35	0.03271541950880419
1104	36-37	0.0752789962154381
1104	38-39	0.05837022164845962
1104	40-41	0.12296640045989449
1104	42-43	-0.06929794271643175
1104	44-45	-0.012838442023038965
1104	46-47	0.11930439171182883
1104	48-49	0.055809263463444836
1104	50-51	-0.07128277648941861
1104	52-53	-0.03895636844093531
1104	54-55	0.1712857506733414
1104	56-57	0.14508461207097412
1104	58-59	0.11394710636072602
1104	60-61	0.029152085609794653
1104	62-63	0.11628114318991578
1104	64-65	0.06749981090072055
1104	66-67	0.1607073222940798
1104	68-69	-0.025864853687615152
1104	70-71	0.09628824659785806
1104	72-73	0.052250936248277924
1104	74-75	0.08123136751498095
1104	76-77	0.012478194792247876
1104	78-79	-0.03806634642156581
1104	80-81	-0.05634193896574757
1104	82-83	-0.035960487104112815
1104	84-85	0.005644661802786999
1104	86-87	-0.06694542709672291
1104	88-89	-0.11050787290880493
1104	90-91	-0.16363459530532154
1104	92-93	-0.1289967688889746
1104	94-95	-0.14414931003972953
1104	96-97	-0.23604703586135045
1104	98-99	-0.20546898290526983
1104	100	-0.19458618419589158
1105	1	0.20451254530337692
1105	2	0.20036216179504152
1105	3	0.16555183978897858
1105	4	0.20133275480158375
1105	5	0.2674014936386442
1105	6	0.31559935265573813
1105	7	0.15731517510632642
1105	8	0.14247175995424755
1105	9	0.2674603244488054
1105	10-11	0.22759401793098988
1105	12-13	0.31358653826411853
1105	14-15	0.24441562794545746
1105	16-17	0.23376741427040315
1105	18-19	0.19243586141197966
1105	20-21	0.15741010856901738
1105	22-23	0.21425292436479282
1105	24-25	0.2181588001269148
1105	26-27	0.20457756288344342
1105	28-29	0.09389183073469098
1105	30-31	0.0786999752546862
1105	32-33	0.12024163980206737
1105	34-35	0.09455282463535752
1105	36-37	0.10791900863407733
1105	38-39	0.03463336372200132
1105	40-41	0.08433117765427056
1105	42-43	0.05783665316338471
1105	44-45	0.00944560089045865
1105	46-47	0.014905577370178946
1105	48-49	0.010082383467505451
1105	50-51	-0.06945672135272929
1105	52-53	0.11051716334784345
1105	54-55	0.16600433890592825
1105	56-57	0.21117924971954238
1105	58-59	0.19473653629905385
1105	60-61	0.1641553571868002
1105	62-63	0.16131294584705103
1105	64-65	0.15020178943092333
1105	66-67	0.2190494055025951
1105	68-69	0.28726926093957417
1105	70-71	0.19495061013925863
1105	72-73	0.13797024654946455
1105	74-75	0.06812743834493062
1105	76-77	0.20721397928940277
1105	78-79	0.22431722124833442
1105	80-81	0.18014492867225584
1105	82-83	0.1373219520386364
1105	84-85	0.07150784410337252
1105	86-87	0.0872865345414553
1105	88-89	0.09963357196284761
1105	90-91	0.025220753343727864
1105	92-93	0.10775634115771027
1105	94-95	0.09238659900800172
1105	96-97	0.140390889379173
1105	98-99	0.16385942690193644
1105	100	0.08433591843268928
1106	1	0.0814790432128163
1106	2	0.09256678391900053
1106	3	0.1811943111096923
1106	4	0.12674322553966277
1106	5	0.06450539607325823
1106	6	0.17004354404640765
1106	7	0.1471109776129751
1106	8	-1.0503379277085614
1106	9	0.09310494464436658
1106	10-11	0.13869607067234568
1106	12-13	0.0894944250242915
1106	14-15	0.0815277088184132
1106	16-17	0.06242497893819632
1106	18-19	0.11970345718039965
1106	20-21	0.061317196997201506
1106	22-23	0.07819959427387602
1106	24-25	0.15972076793774193
1106	26-27	0.18391024097162756
1106	28-29	0.07837880450379942
1106	30-31	0.12508284897205613
1106	32-33	0.12787360875420717
1106	34-35	0.07024760974088906
1106	36-37	0.11070874277401543
1106	38-39	0.08697420239022335
1106	40-41	0.11843905396403187
1106	42-43	0.04531835484002755
1106	44-45	0.01764881485468095
1106	46-47	0.04059117243470922
1106	48-49	0.008469827071479585
1106	50-51	0.08910804778543735
1106	52-53	0.17522817018021897
1106	54-55	0.11834108780380603
1106	56-57	0.03346391044975405
1106	58-59	0.14891716348682849
1106	60-61	0.10738008488635842
1106	62-63	0.09479913296922149
1106	64-65	0.054974380561702674
1106	66-67	0.11882257390464446
1106	68-69	0.10473071182253335
1106	70-71	0.054333254344449244
1106	72-73	0.06832764911830935
1106	74-75	0.16122256294917392
1106	76-77	0.12510898257481173
1106	78-79	0.06379382691679325
1106	80-81	0.09252630236622039
1106	82-83	0.12046407850315433
1106	84-85	0.11188041814015648
1106	86-87	0.048017022643971785
1106	88-89	0.09526566599654984
1106	90-91	0.0024075329718336036
1106	92-93	0.04184800169339553
1106	94-95	-0.006079830812090847
1106	96-97	-0.040784935764147434
1106	98-99	0.03817641136911476
1106	100	-0.025014601048983565
1107	1	1.8857131613714273E-4
1107	2	0.003287228784731866
1107	3	-0.017573064880306077
1107	4	0.1325760382718002
1107	5	0.041843431170427436
1107	6	0.025170972346586495
1107	7	0.17508152205441263
1107	8	0.13797983317039098
1107	9	0.15087517925113758
1107	10-11	0.1250984186631925
1107	12-13	0.08745491637375835
1107	14-15	0.10353979617306663
1107	16-17	0.12330126003164565
1107	18-19	0.09566637780842058
1107	20-21	0.11324077415785894
1107	22-23	0.13097411400008951
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2313	1	0.08867050087081907
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2314	1	-0.5074528543430894
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2314	4	-0.30827646487735905
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2314	52-53	8.830664901751106E-4
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2314	60-61	-0.08978397147540562
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2314	94-95	0.30386599833306605
2314	96-97	0.3091492344301976
2314	98-99	0.3052455239908589
2314	100	0.37141172568966496
2315	1	-0.08030705894001144
2315	2	-0.14986175095286924
2315	3	-0.1597124595574364
2315	4	-0.022566587552475426
2315	5	-0.05822137909101599
2315	6	-0.12040698713174436
2315	7	-0.08108734573893628
2315	8	-0.10571848116257598
2315	9	-0.10170999517706747
2315	10-11	-1.4031164812076256
2315	12-13	-0.09436663316382266
2315	14-15	-0.1787694476631927
2315	16-17	-0.13025051733191617
2315	18-19	-0.036257574407422055
2315	20-21	-0.06292624944070724
2315	22-23	-0.0842028157047281
2315	24-25	-0.10092102826159532
2315	26-27	-0.08593922276116217
2315	28-29	-0.05452725240535017
2315	30-31	-0.005051672469527091
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2315	100	0.4400525965096662
2316	1	-0.2568681735688152
2316	2	-0.40782944959666523
2316	3	-0.344091663692879
2316	4	-0.321710406831869
2316	5	-0.30174671457645275
2316	6	-0.20825811374605507
2316	7	-0.16841019081712005
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2316	10-11	-0.3768501959704622
2316	12-13	-0.35603489375982633
2316	14-15	-0.28948735515329815
2316	16-17	-0.4306492351741795
2316	18-19	-0.3296456689166334
2316	20-21	-0.19734114688060345
2316	22-23	-0.23346832379336746
2316	24-25	-0.22863913575251615
2316	26-27	-0.2734204963750244
2316	28-29	-0.20888852482016773
2316	30-31	-0.2989029640506473
2316	32-33	-0.28568898386907193
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2316	38-39	-0.1640672382352264
2316	40-41	-0.14442768011653584
2316	42-43	-0.1599463300324473
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2316	50-51	-0.06251064393233463
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2316	54-55	-0.08751624431416616
2316	56-57	-0.20297752959328363
2316	58-59	-0.31633388260136996
2316	60-61	-0.24809224850069
2316	62-63	-0.2196770977779181
2316	64-65	-0.4657243843087926
2316	66-67	-0.3677264559076008
2316	68-69	-0.1324039126192318
2316	70-71	-0.15671034405648498
2316	72-73	-0.21976466077163792
2316	74-75	-0.18873953882762962
2316	76-77	-0.2449396195043967
2316	78-79	-0.35834067116289603
2316	80-81	-0.17328639065770446
2316	82-83	-0.15810235724331534
2316	84-85	-0.03280574057903607
2316	86-87	-0.037128910995761544
2316	88-89	0.014470156395191225
2316	90-91	0.08670034708287133
2316	92-93	0.23099140746755964
2316	94-95	0.264679294050957
2316	96-97	0.2563086181422527
2316	98-99	0.2227211930235029
2316	100	-0.11589825053971836
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	26723.0
1	2536.0
2	1556.0
3	1229.0
4	1050.0
5	934.0
6	1087.0
7	1791.0
8	3663.0
9	6348.0
10	12459.0
11	25897.0
12	42232.0
13	54741.0
14	62845.0
15	70133.0
16	79429.0
17	92500.0
18	107701.0
19	124719.0
20	143587.0
21	162845.0
22	183574.0
23	198509.0
24	211334.0
25	225494.0
26	245041.0
27	274347.0
28	316313.0
29	369662.0
30	441411.0
31	538823.0
32	679255.0
33	894230.0
34	1272849.0
35	1961445.0
36	1973102.0
37	1990644.0
38	1687456.0
39	334096.0
40	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.713511260368854	17.229879338352205	12.089134439052204	23.967474962226735
2	16.32748398101775	19.94155620724128	39.64750509460915	24.083454717131815
3	19.891620424078162	25.479290125823194	28.866074051436787	25.763015398661853
4	13.265132114429065	15.464846764477477	36.14744001141183	35.12258110968162
5	13.609060146987293	36.80934420367628	33.57067432740904	16.010921321927395
6	31.811961498319828	34.53783029574124	18.686617444659397	14.963590761279534
7	28.96530901616038	31.62475857372703	21.35727354898718	18.052658861125412
8	27.18548186426799	33.74538193696932	20.053514509728647	19.01562168903404
9	28.11577560757964	15.657239080287786	19.711591860489815	36.51539345164276
10-11	25.93186698413626	25.11795553478045	27.83095312292046	21.119224358162832
12-13	26.337297692058563	22.92768158447829	27.67920039149072	23.05582033197242
14-15	23.626933391771747	24.402166449539838	25.841606793100365	26.129293365588058
16-17	23.269123789780092	27.08826065936341	26.43317163876697	23.209443912089522
18-19	23.01256640762178	26.5983556457532	27.948077402023763	22.441000544601252
20-21	23.796969204075374	25.76626289194052	27.768855229159435	22.667912674824674
22-23	23.450795886294983	25.459292878082085	27.734615888944546	23.355295346678385
24-25	23.366303249312068	25.509359622434662	27.729938764552763	23.394398363700503
26-27	23.138323204741106	25.4104863465612	28.07770127172551	23.373489176972186
28-29	23.014973324130438	26.251280059957004	27.83032529881367	22.90342131709889
30-31	23.84858054190022	25.649697563705566	27.7235681755155	22.77815371887872
32-33	23.539658373607544	25.750132361231376	27.76921895731373	22.940990307847354
34-35	23.620962559207864	25.903237742430374	27.666145814174403	22.809653884187362
36-37	23.63238563826433	25.806675101958753	27.67611535019046	22.884823909586455
38-39	23.523690415290947	26.222903530548358	27.45498304940095	22.798423004759748
40-41	23.450024398732477	25.974884845389074	27.636108960691104	22.938981795187345
42-43	23.223930938290476	26.04086263634469	27.142765217448346	23.59244120791649
44-45	23.104423719616403	26.04671707566929	27.388786617102884	23.46007258761142
46-47	23.22070446835024	25.913076776627932	27.32236915753069	23.54384959749114
48-49	23.26823199060208	26.03941111099615	27.295952370373662	23.396404528028114
50-51	22.936108869438065	26.295433438134086	27.5280797443235	23.240377948104356
52-53	23.107070229536568	26.478192140604822	26.924719248739603	23.490018381119
54-55	22.807130693221932	26.0983306987955	27.36619618135356	23.728342426629006
56-57	23.198252948085848	26.137441072439994	26.978125597324475	23.686180382149683
58-59	23.318412856024604	26.116211175643905	27.158546763253145	23.406829205078346
60-61	23.50228775600425	26.222162113272873	27.36622438689576	22.909325743827118
62-63	23.16687052715732	26.284728631732985	27.35092925683601	23.197471584273682
64-65	23.043331724025702	26.19256053469956	27.18838896041117	23.575718780863568
66-67	23.11154951125227	25.99414091874704	27.030656027233658	23.863653542767025
68-69	22.75665631106341	26.130703149452213	27.215758510227182	23.8968820292572
70-71	22.967781486804757	25.98642660082861	27.24483758168344	23.800954330683197
72-73	22.912497101032262	26.15301779369905	27.057647971686794	23.876837133581898
74-75	22.731297875587604	26.565711662457964	26.871064825588633	23.8319256363658
76-77	22.707347513878887	25.940820103696716	27.071790707289068	24.280041675135333
78-79	22.720877876936623	25.997550607049867	27.240758401976233	24.040813114037274
80-81	22.92343111588416	26.154918508378515	26.895906073126746	24.025744302610573
82-83	22.951171145643038	26.04719662944321	26.998070207486602	24.003562017427154
84-85	22.789044899761198	26.303478849232185	26.814944891213425	24.09253135979319
86-87	22.71902559945802	26.316867893889757	26.8892954039762	24.074811102676026
88-89	22.4983166500209	25.99692778903845	27.001288837554576	24.503466723386072
90-91	23.047677779022127	25.715453513114372	27.03903858033236	24.19783012753114
92-93	22.991916728188457	25.92446277196952	27.030346771207554	24.053273728634473
94-95	22.83626495505914	25.66567369347131	27.05546973540145	24.442591616068103
96-97	22.537944564939888	25.631764508351303	27.26159862420976	24.568692302499052
98-99	22.65275283521191	25.713785384051334	26.90671885818959	24.72674292254717
100	22.606278636702676	25.449610397078697	27.085323631738557	24.85878733448007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12160.0
1	13602.5
2	14279.0
3	10442.0
4	5861.5
5	4670.5
6	6146.5
7	10041.0
8	15245.0
9	17772.0
10	16056.0
11	13876.5
12	14036.5
13	15009.5
14	16075.0
15	17039.5
16	17798.5
17	18830.0
18	19984.5
19	21147.5
20	22579.5
21	24713.0
22	27858.5
23	32494.0
24	38032.5
25	44741.5
26	54599.0
27	66940.5
28	79732.0
29	95901.0
30	116746.5
31	136437.5
32	159056.0
33	185940.5
34	212896.0
35	238908.0
36	271114.0
37	303092.5
38	324776.0
39	346494.0
40	365018.5
41	382035.5
42	403439.0
43	430442.5
44	457777.5
45	484869.0
46	516139.0
47	561665.0
48	639014.0
49	675614.5
50	669509.0
51	655840.5
52	619447.0
53	592396.0
54	571985.0
55	542086.0
56	498599.5
57	453740.5
58	412246.0
59	360851.5
60	304002.0
61	253100.0
62	209419.0
63	167926.5
64	127503.5
65	96021.0
66	73071.0
67	56400.0
68	43755.5
69	33669.5
70	26378.0
71	20609.0
72	16040.0
73	12428.0
74	9564.5
75	7174.0
76	5295.0
77	3628.5
78	2380.5
79	1586.0
80	1131.5
81	795.0
82	529.5
83	391.0
84	290.0
85	222.0
86	170.0
87	131.0
88	112.5
89	91.0
90	65.5
91	51.5
92	39.0
93	32.0
94	25.0
95	19.0
96	17.0
97	10.0
98	9.0
99	9.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1465475352942847
2	0.07185713247812192
3	0.313926353454927
4	0.3117609254108529
5	0.31386564051911175
6	0.26132196751196834
7	0.2617874333532179
8	0.26113308282276565
9	0.2722975171309981
10-11	0.24913215917663545
12-13	0.25852579952359234
14-15	0.23273292062478468
16-17	0.24145197279601757
18-19	0.2550719080638854
20-21	0.25616811384943694
22-23	0.24627865119332287
24-25	0.24683518643829516
26-27	0.24575584535713674
28-29	0.25587466799299696
30-31	0.24140812456459548
32-33	0.253769952884738
34-35	0.2380149460407037
36-37	0.2454455347963037
38-39	0.2470578005362841
40-41	0.2649748624835139
42-43	0.24976964500269463
44-45	0.2349860451317029
46-47	0.2381464907349699
48-49	0.24797861339614738
50-51	0.24148232926392513
52-53	0.25841111953371926
54-55	0.2684759751155215
56-57	0.26431039313042576
58-59	0.2610487593008001
60-61	0.24215354449877052
62-63	0.27021303966801086
64-65	0.26331200263035426
66-67	0.268489466879036
68-69	0.2735994723101454
70-71	0.293800015232201
72-73	0.30283275090514555
74-75	0.28194412804385144
76-77	0.27943803297103675
78-79	0.27666547556881105
80-81	0.26305903206445774
82-83	0.27431116283553425
84-85	0.284895451312644
86-87	0.2767599179134124
88-89	0.28549920772991705
90-91	0.27974834353186984
92-93	0.2593622888614901
94-95	0.2809929587160806
96-97	0.27909736594229617
98-99	0.2653694965663124
100	0.28861580510176194
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.4823859E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.449271572974105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.5444300760137	33.20927707567522
2	16.220713969600055	15.717635524413758
3	6.527202505763577	9.487146204406098
4	3.0864989465249284	5.9815450267953905
5	1.6597347088037822	4.020646882296278
6	1.0164237899632362	2.954699533989625
7	0.6355249494984964	2.1553504608757312
8	0.4450347468805372	1.7249287448819948
9	0.32183994340597455	1.4033619738996008
>10	1.4205025008072947	11.874824022387266
>50	0.07332988374981268	2.4193070921602935
>100	0.043038764827094095	4.042740860454254
>500	0.0035819559258271397	1.1762582699497557
>1k	0.0018118267989760952	1.3965420316567305
>5k	1.9885886202278253E-4	0.6560390779166256
>10k+	1.3257257468185502E-4	1.7796972182413262
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45961	0.3100474714445139	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36123	0.24368148671678544	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	34380	0.23192341481391587	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23475	0.1583595742512122	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0237645271720408E-5	0.0	0.0	0.0
3	0.0	2.0237645271720408E-5	0.0	0.0	0.0
4	0.0	2.0237645271720408E-5	0.0	0.0	1.349176351448027E-5
5	0.0	2.0237645271720408E-5	0.0	0.0	1.349176351448027E-5
6	0.0	2.0237645271720408E-5	0.0	0.0	4.0475290543440816E-5
7	0.0	2.0237645271720408E-5	0.0	6.745881757240135E-6	4.722117230068095E-5
8	0.0	2.0237645271720408E-5	0.0	6.745881757240135E-6	9.44423446013619E-5
9	0.0	2.0237645271720408E-5	0.0	1.349176351448027E-5	1.1467998987308231E-4
10-11	0.0	2.0237645271720408E-5	0.0	1.349176351448027E-5	1.2142587163032244E-4
12-13	0.0	2.0237645271720408E-5	0.0	1.686470439310034E-5	1.2142587163032244E-4
14-15	0.0	2.698352702896054E-5	0.0	3.710234966482075E-5	1.5178233953790305E-4
16-17	0.0	3.372940878620068E-5	0.0	5.7339994936541155E-5	1.9563057095996393E-4
18-19	0.0	3.372940878620068E-5	0.0	8.432352196550169E-5	2.2261409798892448E-4
20-21	0.0	3.372940878620068E-5	0.0	1.2479881250894253E-4	2.394788023820248E-4
22-23	0.0	3.372940878620068E-5	0.0	3.2717526522614657E-4	3.204293834689065E-4
24-25	0.0	3.710234966482075E-5	0.0	7.791493429612356E-4	3.878882010413078E-4
26-27	0.0	4.0475290543440816E-5	0.0	0.0017100810254603745	4.114987871916483E-4
28-29	0.0	4.0475290543440816E-5	0.0	0.004985206618600461	4.148717280702684E-4
30-31	0.0	4.722117230068095E-5	0.0	0.016203607980890804	4.1824466894888843E-4
32-33	0.0	4.722117230068095E-5	0.0	0.04440476666703319	4.4860113685646905E-4
34-35	0.0	4.722117230068095E-5	0.0	0.08756491814985558	4.9919525003577E-4
36-37	0.0	6.071293581516122E-5	0.0	0.15349916644512068	5.396705405792109E-4
38-39	0.0	6.745881757240136E-5	0.0	0.25339555644721123	5.46416422336451E-4
40-41	0.0	6.745881757240136E-5	0.0	0.3756410527110383	5.632811267295513E-4
42-43	0.0	7.083175845102143E-5	0.0	0.48722805579842604	5.835187720012718E-4
44-45	0.0	7.420469932964149E-5	0.0	0.6209719075174689	6.037564172729922E-4
46-47	0.0	7.420469932964149E-5	0.0	0.7819050356590682	6.341128851805728E-4
48-49	0.0	7.757764020826156E-5	0.0	0.9472769539969316	7.48792875053655E-4
50-51	0.0	8.432352196550169E-5	0.0	1.1217322021209188	7.555387568108952E-4
52-53	0.0	8.769646284412176E-5	0.0	1.3027242096676717	8.162516926260564E-4
54-55	0.0	8.769646284412176E-5	0.0	1.5095158419949892	9.140669781060384E-4
56-57	0.0	8.769646284412176E-5	0.0	1.7490148820222857	9.41050505134999E-4
58-59	0.0	8.769646284412176E-5	0.0	2.007992655623613	9.511693277708591E-4
60-61	0.0	8.769646284412176E-5	0.0	2.2745291897339284	9.545422686494792E-4
62-63	0.0	8.769646284412176E-5	0.0	2.557080447135931	9.815257956784398E-4
64-65	0.0	9.106940372274183E-5	0.0	2.836488123639061	0.0010017634409501602
66-67	0.0	9.44423446013619E-5	0.0	3.1505730053152825	0.0010186281453432606
68-69	0.0	9.44423446013619E-5	0.0	3.509615815962632	0.0010253740271005006
70-71	0.0	1.1130704899446224E-4	0.0	3.8998111085649154	0.0010725951994011815
72-73	0.0	1.3491763514480271E-4	0.0	4.261228469590813	0.001194021071031504
74-75	0.0	1.3491763514480271E-4	0.0	4.631712295698442	0.001194021071031504
76-77	0.0	1.5515528041652312E-4	0.0	5.035645576499345	0.0012108857754246044
78-79	0.0	1.5515528041652312E-4	0.0	5.489306124673744	0.0012210045980604646
80-81	0.0	1.5515528041652312E-4	0.0	5.948481431184687	0.0012513610659680451
82-83	0.0	1.585282212951432E-4	0.0	6.421984990548008	0.001349176351448027
84-85	0.0	1.6190116217376326E-4	0.0	6.9102316744917776	0.0013559222332052672
86-87	0.0	1.6190116217376326E-4	0.0	7.411626081980407	0.0013761598784769876
88	0.0	1.6190116217376326E-4	0.0	7.808627969275746	0.0013761598784769876
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	24725	0.0	53.21716	2
GTACCTG	29320	0.0	45.498825	1
ACCTGGG	28955	0.0	44.955647	3
CCTGGGG	26080	0.0	33.535023	4
CATGGGG	41310	0.0	25.792767	4
GTACATG	68775	0.0	25.251608	1
TGGGGGG	26230	0.0	25.117867	6
ATGGGGG	25675	0.0	24.411049	5
TACATGG	70605	0.0	24.217466	2
TATAACG	1355	0.0	23.252829	2
ACATGGG	72205	0.0	23.159876	3
CTGGGGG	17250	0.0	22.70506	5
TATCACG	1445	0.0	21.479115	2
GTACCCG	2995	0.0	21.20288	1
TTAAGAG	12905	0.0	18.945763	5
GAGTACT	39495	0.0	18.70907	12-13
GTACACG	3415	0.0	17.63101	1
GTATCAA	97810	0.0	17.616175	1
ATAACGC	1855	0.0	17.4923	3
AGTACTT	41930	0.0	17.415253	12-13
>>END_MODULE