FastQCFastQC Report
Mon 6 Jun 2016
ERR522860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14823859
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410410.2768577331988924No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT363910.24548938302772577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353580.23852088717249673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231940.15646398147742768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG253450.053.679622
GTACCTG295500.046.2852971
ACCTGGG292500.045.5944333
CCTGGGG253850.035.079824
TATAACG11550.028.0750312
TGGGGGG235950.024.6762716
CTGGGGG157750.024.575845
CATGGGG410550.023.6591824
GTACATG696800.023.312921
ATGGGGG250100.022.6786615
TACATGG714350.022.5255722
ACATGGG717450.021.7259653
TATCACG13900.020.285682
GTACCCG27950.019.0087451
GAGTACT400700.018.41763312-13
TAACGCA19400.018.1670674
ATAACGC19200.018.1115593
GTACACG34700.017.479021
AGTACTT422800.017.27148812-13
GTATAGG40300.016.8001921
Produced by FastQC (version 0.11.5)